SrasV1, PubMed, Curation, bibRecord, 003223

Mechanisms and enzymes involved in SARS coronavirus genome expression.

Identifieur interne : 003223 ( PubMed/Curation ); précédent : 003222; suivant : 003224

Mechanisms and enzymes involved in SARS coronavirus genome expression.

Auteurs : Volker Thiel [Allemagne] ; Konstantin A. Ivanov [Allemagne] ; Ákos Putics [Allemagne] ; Tobias Hertzig [Allemagne] ; Barbara Schelle [Allemagne] ; Sonja Bayer [Allemagne] ; Benedikt Wei Brich [Allemagne] ; Eric J. Snijder [Pays-Bas] ; Holger Rabenau [Allemagne] ; Hans Wilhelm Doerr [Allemagne] ; Alexander E. Gorbalenya [Pays-Bas] ; John Ziebuhr [Allemagne]

Source :

The Journal of general virology [ 0022-1317 ] ; 2003.

RBID : pubmed:12917450

Descripteurs français

KwdFr :
- ARN messager (), ARN messager (génétique), Alignement de séquences, Biosynthèse des protéines, Conformation d'acide nucléique, Cysteine endopeptidases (génétique), Cysteine endopeptidases (métabolisme), Domaine catalytique, Données de séquences moléculaires, Décalage ribosomique du cadre de lecture, Génome viral, Papaïne (génétique), Papaïne (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), RNA helicases (biosynthèse), RNA helicases (génétique), Régulation de l'expression des gènes viraux, Séquence d'acides aminés, Virus du SRAS (enzymologie), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- biosynthèse : RNA helicases.
- enzymologie : Virus du SRAS.
- génétique : ARN messager, Cysteine endopeptidases, Papaïne, Protéines virales, RNA helicases, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- métabolisme : Cysteine endopeptidases, Papaïne, Protéines virales.
- ARN messager, Alignement de séquences, Biosynthèse des protéines, Conformation d'acide nucléique, Domaine catalytique, Données de séquences moléculaires, Décalage ribosomique du cadre de lecture, Génome viral, Régulation de l'expression des gènes viraux, Séquence d'acides aminés.

English descriptors

KwdEn :
- Amino Acid Sequence, Catalytic Domain, Cysteine Endopeptidases (genetics), Cysteine Endopeptidases (metabolism), Frameshifting, Ribosomal, Gene Expression Regulation, Viral, Genome, Viral, Molecular Sequence Data, Nucleic Acid Conformation, Papain (genetics), Papain (metabolism), Protein Biosynthesis, RNA Helicases (biosynthesis), RNA Helicases (genetics), RNA, Messenger (chemistry), RNA, Messenger (genetics), SARS Virus (enzymology), SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Alignment, Viral Proteins (genetics), Viral Proteins (metabolism).
MESH :
- chemical , biosynthesis : RNA Helicases.
- chemical , chemistry : RNA, Messenger.
- chemical , genetics : Cysteine Endopeptidases, Papain, RNA Helicases, RNA, Messenger, Viral Proteins.
- chemical , metabolism : Cysteine Endopeptidases, Papain, Viral Proteins.
- enzymology : SARS Virus.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- Amino Acid Sequence, Catalytic Domain, Frameshifting, Ribosomal, Gene Expression Regulation, Viral, Genome, Viral, Molecular Sequence Data, Nucleic Acid Conformation, Protein Biosynthesis, Sequence Alignment.

Abstract

A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavirus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.

DOI: 10.1099/vir.0.19424-0
PubMed: 12917450

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<term>Conformation d'acide nucléique</term>
<term>Domaine catalytique</term>
<term>Données de séquences moléculaires</term>
<term>Décalage ribosomique du cadre de lecture</term>
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<front><div type="abstract" xml:lang="en">A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavirus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.</div>
</front>
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<Month>10</Month>
<Day>16</Day>
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<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>25</Day>
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<Month>Sep</Month>
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<Title>The Journal of general virology</Title>
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</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.19424-0</ELocationID>
<Abstract><AbstractText>A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavirus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Thiel</LastName>
<ForeName>Volker</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ivanov</LastName>
<ForeName>Konstantin A</ForeName>
<Initials>KA</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Putics</LastName>
<ForeName>Ákos</ForeName>
<Initials>Á</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hertzig</LastName>
<ForeName>Tobias</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schelle</LastName>
<ForeName>Barbara</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bayer</LastName>
<ForeName>Sonja</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Weißbrich</LastName>
<ForeName>Benedikt</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Snijder</LastName>
<ForeName>Eric J</ForeName>
<Initials>EJ</Initials>
<AffiliationInfo><Affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rabenau</LastName>
<ForeName>Holger</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt (Main), Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Doerr</LastName>
<ForeName>Hans Wilhelm</ForeName>
<Initials>HW</Initials>
<AffiliationInfo><Affiliation>Institute for Medical Virology, Johann Wolfgang Goethe University, Frankfurt (Main), Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gorbalenya</LastName>
<ForeName>Alexander E</ForeName>
<Initials>AE</Initials>
<AffiliationInfo><Affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ziebuhr</LastName>
<ForeName>John</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Institute of Virology and Immunology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName>
<AccessionNumberList><AccessionNumber>AY291315</AccessionNumber>
</AccessionNumberList>
</DataBank>
</DataBankList>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Gen Virol</MedlineTA>
<NlmUniqueID>0077340</NlmUniqueID>
<ISSNLinking>0022-1317</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber>
<NameOfSubstance UI="C099456">3C-like proteinase, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber>
<NameOfSubstance UI="D003546">Cysteine Endopeptidases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.22.2</RegistryNumber>
<NameOfSubstance UI="D010206">Papain</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.22.2</RegistryNumber>
<NameOfSubstance UI="C000657884">papain-like protease, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.6.4.13</RegistryNumber>
<NameOfSubstance UI="D020365">RNA Helicases</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020134" MajorTopicYN="N">Catalytic Domain</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003546" MajorTopicYN="N">Cysteine Endopeptidases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018965" MajorTopicYN="N">Frameshifting, Ribosomal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015967" MajorTopicYN="Y">Gene Expression Regulation, Viral</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009690" MajorTopicYN="N">Nucleic Acid Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010206" MajorTopicYN="N">Papain</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020365" MajorTopicYN="N">RNA Helicases</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year>
<Month>8</Month>
<Day>15</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2003</Year>
<Month>10</Month>
<Day>17</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2003</Year>
<Month>8</Month>
<Day>15</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">12917450</ArticleId>
<ArticleId IdType="doi">10.1099/vir.0.19424-0</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

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Mechanisms and enzymes involved in SARS coronavirus genome expression.

Mechanisms and enzymes involved in SARS coronavirus genome expression.

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