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Piezoelectric immunosensor for SARS-associated coronavirus in sputum.

Identifieur interne : 002C86 ( PubMed/Curation ); précédent : 002C85; suivant : 002C87

Piezoelectric immunosensor for SARS-associated coronavirus in sputum.

Auteurs : Boli Zuo [République populaire de Chine] ; Shanmao Li ; Zhao Guo ; Jinfang Zhang ; Chuanzhi Chen

Source :

Analytical chemistry [ 0003-2700 ] ; 2004.

RBID : pubmed:15228322

Descripteurs français

KwdFr :
- Adsorption, Dosage immunologique (), Dosage immunologique (instrumentation), Expectoration (virologie), Humains, Propriétés de surface, Science des ultrasons, Techniques de biocapteur (), Techniques de biocapteur (instrumentation), Virus du SRAS (), Virus du SRAS (immunologie).
MESH :
- immunologie : Virus du SRAS.
- virologie : Expectoration.
- Adsorption, Dosage immunologique, Humains, Propriétés de surface, Science des ultrasons, Techniques de biocapteur, Virus du SRAS.

English descriptors

KwdEn :
- Adsorption, Biosensing Techniques (instrumentation), Biosensing Techniques (methods), Humans, Immunoassay (instrumentation), Immunoassay (methods), SARS Virus (chemistry), SARS Virus (immunology), Sputum (virology), Surface Properties, Ultrasonics.
MESH :
- chemistry : SARS Virus.
- immunology : SARS Virus.
- instrumentation : Biosensing Techniques, Immunoassay.
- methods : Biosensing Techniques, Immunoassay.
- virology : Sputum.
- Adsorption, Humans, Surface Properties, Ultrasonics.

Abstract

A piezoelectric immunosensor was developed for the detection of SARS-associated coronavirus (SARS-CoV) in sputum in the gas phase. Horse polyclonal antibody against SARS-CoV was bound onto the PZ crystal surface in an ordered orientation through protein A. The antigen sample was atomized into aerosol by an ultrasonator, by which the antibody on the crystal could specifically adsorb SARS antigen and the changed mass of crystal would lead a frequency shift. A frequency counter was employed to record the admittance frequency, and the plot of changed frequency was displayed on the computer. Under the optimized conditions, the frequency shifts were linearly dependent on antigen concentration in the range of 0.6-4 microg/mL. The device has good reproducibility (could be reused 100 times without detectable loss of activity), stability (the immunosensor was stable for more than two months when stored over silica gel blue at 4-6 degrees C), short analyzing time (less than 2 min), and specificity.

DOI: 10.1021/ac035367b
PubMed: 15228322

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pubmed:15228322

Le document en format XML

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<author><name sortKey="Zuo, Boli" sort="Zuo, Boli" uniqKey="Zuo B" first="Boli" last="Zuo">Boli Zuo</name>
<affiliation wicri:level="1"><nlm:affiliation>The Analytical Center, Institute of Chemical Defense of PLA, 1048#, Beijing 102205, P. R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Analytical Center, Institute of Chemical Defense of PLA, 1048#, Beijing 102205</wicri:regionArea>
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<author><name sortKey="Li, Shanmao" sort="Li, Shanmao" uniqKey="Li S" first="Shanmao" last="Li">Shanmao Li</name>
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<author><name sortKey="Guo, Zhao" sort="Guo, Zhao" uniqKey="Guo Z" first="Zhao" last="Guo">Zhao Guo</name>
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<author><name sortKey="Zhang, Jinfang" sort="Zhang, Jinfang" uniqKey="Zhang J" first="Jinfang" last="Zhang">Jinfang Zhang</name>
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<author><name sortKey="Chen, Chuanzhi" sort="Chen, Chuanzhi" uniqKey="Chen C" first="Chuanzhi" last="Chen">Chuanzhi Chen</name>
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<author><name sortKey="Guo, Zhao" sort="Guo, Zhao" uniqKey="Guo Z" first="Zhao" last="Guo">Zhao Guo</name>
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<author><name sortKey="Zhang, Jinfang" sort="Zhang, Jinfang" uniqKey="Zhang J" first="Jinfang" last="Zhang">Jinfang Zhang</name>
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<author><name sortKey="Chen, Chuanzhi" sort="Chen, Chuanzhi" uniqKey="Chen C" first="Chuanzhi" last="Chen">Chuanzhi Chen</name>
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<series><title level="j">Analytical chemistry</title>
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<term>Biosensing Techniques (methods)</term>
<term>Humans</term>
<term>Immunoassay (instrumentation)</term>
<term>Immunoassay (methods)</term>
<term>SARS Virus (chemistry)</term>
<term>SARS Virus (immunology)</term>
<term>Sputum (virology)</term>
<term>Surface Properties</term>
<term>Ultrasonics</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adsorption</term>
<term>Dosage immunologique ()</term>
<term>Dosage immunologique (instrumentation)</term>
<term>Expectoration (virologie)</term>
<term>Humains</term>
<term>Propriétés de surface</term>
<term>Science des ultrasons</term>
<term>Techniques de biocapteur ()</term>
<term>Techniques de biocapteur (instrumentation)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Immunoassay</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
<term>Immunoassay</term>
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<term>Surface Properties</term>
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<term>Dosage immunologique</term>
<term>Humains</term>
<term>Propriétés de surface</term>
<term>Science des ultrasons</term>
<term>Techniques de biocapteur</term>
<term>Virus du SRAS</term>
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<front><div type="abstract" xml:lang="en">A piezoelectric immunosensor was developed for the detection of SARS-associated coronavirus (SARS-CoV) in sputum in the gas phase. Horse polyclonal antibody against SARS-CoV was bound onto the PZ crystal surface in an ordered orientation through protein A. The antigen sample was atomized into aerosol by an ultrasonator, by which the antibody on the crystal could specifically adsorb SARS antigen and the changed mass of crystal would lead a frequency shift. A frequency counter was employed to record the admittance frequency, and the plot of changed frequency was displayed on the computer. Under the optimized conditions, the frequency shifts were linearly dependent on antigen concentration in the range of 0.6-4 microg/mL. The device has good reproducibility (could be reused 100 times without detectable loss of activity), stability (the immunosensor was stable for more than two months when stored over silica gel blue at 4-6 degrees C), short analyzing time (less than 2 min), and specificity.</div>
</front>
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<Month>05</Month>
<Day>02</Day>
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<DateRevised><Year>2006</Year>
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<Day>15</Day>
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<Title>Analytical chemistry</Title>
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<ArticleTitle>Piezoelectric immunosensor for SARS-associated coronavirus in sputum.</ArticleTitle>
<Pagination><MedlinePgn>3536-40</MedlinePgn>
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<Abstract><AbstractText>A piezoelectric immunosensor was developed for the detection of SARS-associated coronavirus (SARS-CoV) in sputum in the gas phase. Horse polyclonal antibody against SARS-CoV was bound onto the PZ crystal surface in an ordered orientation through protein A. The antigen sample was atomized into aerosol by an ultrasonator, by which the antibody on the crystal could specifically adsorb SARS antigen and the changed mass of crystal would lead a frequency shift. A frequency counter was employed to record the admittance frequency, and the plot of changed frequency was displayed on the computer. Under the optimized conditions, the frequency shifts were linearly dependent on antigen concentration in the range of 0.6-4 microg/mL. The device has good reproducibility (could be reused 100 times without detectable loss of activity), stability (the immunosensor was stable for more than two months when stored over silica gel blue at 4-6 degrees C), short analyzing time (less than 2 min), and specificity.</AbstractText>
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<MeshHeading><DescriptorName UI="D013183" MajorTopicYN="N">Sputum</DescriptorName>
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<MeshHeading><DescriptorName UI="D013499" MajorTopicYN="N">Surface Properties</DescriptorName>
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Piezoelectric immunosensor for SARS-associated coronavirus in sputum.

Piezoelectric immunosensor for SARS-associated coronavirus in sputum.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

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Pour générer des pages wiki