[Comparison among four kits in detection of anti-SARS-CoV IgG in sera of SARS patients].
Identifieur interne : 002C82 ( PubMed/Curation ); précédent : 002C81; suivant : 002C83[Comparison among four kits in detection of anti-SARS-CoV IgG in sera of SARS patients].
Auteurs : Xue-En Liu [République populaire de Chine] ; Jie Li ; Yong-Hua Li ; Ling Wang ; Tong Li ; Hai-Ying Lu ; Guang-Fa Wang ; Wan-Fu Zhu ; Xiao-Ming Gao ; You-Chun Wang ; Zhen-Dong Zhao ; Xiao-Yuan Xu ; Hui ZhuangSource :
- Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi [ 0254-6450 ] ; 2004.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Femelle, Humains, Immunoglobuline G (sang), Mâle, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Technique d'immunofluorescence indirecte, Test ELISA, Trousses de réactifs pour diagnostic, Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G.
- virologie : Syndrome respiratoire aigu sévère.
- Femelle, Humains, Mâle, Sensibilité et spécificité, Technique d'immunofluorescence indirecte, Test ELISA, Trousses de réactifs pour diagnostic.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G (blood), Male, Reagent Kits, Diagnostic, SARS Virus (immunology), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Male, Reagent Kits, Diagnostic, Sensitivity and Specificity.
Abstract
To compare the sensitivity and specificity of four kits for detection of anti-severe acute respiratory syndrome (SARS)-CoV IgG in sera of SARS patients.
PubMed: 15231132
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pubmed:15231132Le document en format XML
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Wang</name></author><author><name sortKey="Li, Tong" sort="Li, Tong" uniqKey="Li T" first="Tong" last="Li">Tong Li</name></author><author><name sortKey="Lu, Hai Ying" sort="Lu, Hai Ying" uniqKey="Lu H" first="Hai-Ying" last="Lu">Hai-Ying Lu</name></author><author><name sortKey="Wang, Guang Fa" sort="Wang, Guang Fa" uniqKey="Wang G" first="Guang-Fa" last="Wang">Guang-Fa Wang</name></author><author><name sortKey="Zhu, Wan Fu" sort="Zhu, Wan Fu" uniqKey="Zhu W" first="Wan-Fu" last="Zhu">Wan-Fu Zhu</name></author><author><name sortKey="Gao, Xiao Ming" sort="Gao, Xiao Ming" uniqKey="Gao X" first="Xiao-Ming" last="Gao">Xiao-Ming Gao</name></author><author><name sortKey="Wang, You Chun" sort="Wang, You Chun" uniqKey="Wang Y" first="You-Chun" last="Wang">You-Chun Wang</name></author><author><name sortKey="Zhao, Zhen Dong" sort="Zhao, Zhen Dong" uniqKey="Zhao Z" first="Zhen-Dong" last="Zhao">Zhen-Dong Zhao</name></author><author><name sortKey="Xu, Xiao Yuan" sort="Xu, Xiao Yuan" uniqKey="Xu X" first="Xiao-Yuan" last="Xu">Xiao-Yuan Xu</name></author><author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15231132</idno><idno type="pmid">15231132</idno><idno type="wicri:Area/PubMed/Corpus">002C82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C82</idno><idno type="wicri:Area/PubMed/Curation">002C82</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C82</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Comparison among four kits in detection of anti-SARS-CoV IgG in sera of SARS patients].</title><author><name sortKey="Liu, Xue En" sort="Liu, Xue En" uniqKey="Liu X" first="Xue-En" last="Liu">Xue-En Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Peking University Health Science Center, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, Peking University Health Science Center, Beijing 100083</wicri:regionArea></affiliation></author><author><name sortKey="Li, Jie" sort="Li, Jie" uniqKey="Li J" first="Jie" last="Li">Jie Li</name></author><author><name sortKey="Li, Yong Hua" sort="Li, Yong Hua" uniqKey="Li Y" first="Yong-Hua" last="Li">Yong-Hua Li</name></author><author><name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name></author><author><name sortKey="Li, Tong" sort="Li, Tong" uniqKey="Li T" first="Tong" last="Li">Tong Li</name></author><author><name sortKey="Lu, Hai Ying" sort="Lu, Hai Ying" uniqKey="Lu H" first="Hai-Ying" last="Lu">Hai-Ying Lu</name></author><author><name sortKey="Wang, Guang Fa" sort="Wang, Guang Fa" uniqKey="Wang G" first="Guang-Fa" last="Wang">Guang-Fa Wang</name></author><author><name sortKey="Zhu, Wan Fu" sort="Zhu, Wan Fu" uniqKey="Zhu W" first="Wan-Fu" last="Zhu">Wan-Fu Zhu</name></author><author><name sortKey="Gao, Xiao Ming" sort="Gao, Xiao Ming" uniqKey="Gao X" first="Xiao-Ming" last="Gao">Xiao-Ming Gao</name></author><author><name sortKey="Wang, You Chun" sort="Wang, You Chun" uniqKey="Wang Y" first="You-Chun" last="Wang">You-Chun Wang</name></author><author><name sortKey="Zhao, Zhen Dong" sort="Zhao, Zhen Dong" uniqKey="Zhao Z" first="Zhen-Dong" last="Zhao">Zhen-Dong Zhao</name></author><author><name sortKey="Xu, Xiao Yuan" sort="Xu, Xiao Yuan" uniqKey="Xu X" first="Xiao-Yuan" last="Xu">Xiao-Yuan Xu</name></author><author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name></author></analytic><series><title level="j">Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi</title><idno type="ISSN">0254-6450</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Male</term><term>Reagent Kits, Diagnostic</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term><term>Femelle</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Mâle</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique d'immunofluorescence indirecte</term><term>Test ELISA</term><term>Trousses de réactifs pour diagnostic</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>Male</term><term>Reagent Kits, Diagnostic</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Sensibilité et spécificité</term><term>Technique d'immunofluorescence indirecte</term><term>Test ELISA</term><term>Trousses de réactifs pour diagnostic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To compare the sensitivity and specificity of four kits for detection of anti-severe acute respiratory syndrome (SARS)-CoV IgG in sera of SARS patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15231132</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>09</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0254-6450</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>6</Issue><PubDate><Year>2004</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi</Title><ISOAbbreviation>Zhonghua Liu Xing Bing Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Comparison among four kits in detection of anti-SARS-CoV IgG in sera of SARS patients].</ArticleTitle><Pagination><MedlinePgn>514-6</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To compare the sensitivity and specificity of four kits for detection of anti-severe acute respiratory syndrome (SARS)-CoV IgG in sera of SARS patients.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Anti-SARS-CoV IgG was detected in 99 serial sera from 18 SARS patients and in 123 negative reference sera, using two enzyme linked immunosorbent assays (EIA No. A and No. B) and two indirect immunofluorescence assays (Australian IFA and Euroimmun IFA).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Anti-SARS-CoV IgG was not detected in sera collected from SARS patients at the first week after onset by any of the four kits, however, it was detectable in sera obtained at the second week of illness by EIA No. B, and two IFA, but not by EIA No. A, with the positive rates of 57.1% (4/7), 57.1% (4/7) and 42.9% (3/7), respectively. The anti-SARS-CoV IgG was first determined in sera on the 9th day by Euroimmun IFA, 12th day by EIA No. B, 13th day by Australian IFA, and 16th day by EIA No. A. The positive rates of antibody on the 3rd week after onset were 84.2% (16/19), 94.7% (18/19), 78.9% (15/19) and 52.6% (10/19) respectively. They were identical since the 4th week after the disease onset. Through detection of 123 negative reference sera, the specificity of EIA No. A and two IFA was 100%, with exception of 94.9% for EIA No. B.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The sensitivity and specificity of the two IFAs were relatively higher than that of the two EIAs. The quality of the two homemade EIAs should be improved.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xue-En</ForeName><Initials>XE</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Peking University Health Science Center, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yong-Hua</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ling</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Tong</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Hai-Ying</ForeName><Initials>HY</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Guang-Fa</ForeName><Initials>GF</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Wan-Fu</ForeName><Initials>WF</Initials></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Xiao-Ming</ForeName><Initials>XM</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>You-Chun</ForeName><Initials>YC</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhen-Dong</ForeName><Initials>ZD</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Xiao-Yuan</ForeName><Initials>XY</Initials></Author><Author ValidYN="Y"><LastName>Zhuang</LastName><ForeName>Hui</ForeName><Initials>H</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Liu Xing Bing Xue Za Zhi</MedlineTA><NlmUniqueID>8208604</NlmUniqueID><ISSNLinking>0254-6450</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011933">Reagent Kits, Diagnostic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019084" MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011933" MajorTopicYN="Y">Reagent Kits, Diagnostic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>7</Month><Day>3</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>6</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>7</Month><Day>3</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15231132</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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