Determination of SARS-coronavirus by a microfluidic chip system.
Identifieur interne : 002B79 ( PubMed/Curation ); précédent : 002B78; suivant : 002B80Determination of SARS-coronavirus by a microfluidic chip system.
Auteurs : Xiaomian Zhou [République populaire de Chine] ; Dayu Liu ; Runtao Zhong ; Zhongpeng Dai ; Dapeng Wu ; Hui Wang ; Yuguang Du ; Zhinan Xia ; Liping Zhang ; Xiaodai Mei ; Bingcheng LinSource :
- Electrophoresis [ 0173-0835 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Virus du SRAS.
- virologie : Poumon.
- Autopsie, Humains, Laboratoires sur puces, Procédures d'analyse sur micropuce, Réaction de polymérisation en chaîne, Électrophorèse capillaire.
English descriptors
- KwdEn :
- MESH :
- isolation & purification : SARS Virus.
- methods : Electrophoresis, Capillary, Microchip Analytical Procedures.
- virology : Lung.
- Autopsy, Humans, Lab-On-A-Chip Devices, Polymerase Chain Reaction.
Abstract
We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-CoV). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
DOI: 10.1002/elps.200305966
PubMed: 15349945
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name></author><author><name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name></author><author><name sortKey="Dai, Zhongpeng" sort="Dai, Zhongpeng" uniqKey="Dai Z" first="Zhongpeng" last="Dai">Zhongpeng Dai</name></author><author><name sortKey="Wu, Dapeng" sort="Wu, Dapeng" uniqKey="Wu D" first="Dapeng" last="Wu">Dapeng Wu</name></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name></author><author><name sortKey="Du, Yuguang" sort="Du, Yuguang" uniqKey="Du Y" first="Yuguang" last="Du">Yuguang Du</name></author><author><name sortKey="Xia, Zhinan" sort="Xia, Zhinan" uniqKey="Xia Z" first="Zhinan" last="Xia">Zhinan Xia</name></author><author><name sortKey="Zhang, Liping" sort="Zhang, Liping" uniqKey="Zhang L" first="Liping" last="Zhang">Liping Zhang</name></author><author><name sortKey="Mei, Xiaodai" sort="Mei, Xiaodai" uniqKey="Mei X" first="Xiaodai" last="Mei">Xiaodai Mei</name></author><author><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15349945</idno><idno type="pmid">15349945</idno><idno type="doi">10.1002/elps.200305966</idno><idno type="wicri:Area/PubMed/Corpus">002B79</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B79</idno><idno type="wicri:Area/PubMed/Curation">002B79</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B79</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Determination of SARS-coronavirus by a microfluidic chip system.</title><author><name sortKey="Zhou, Xiaomian" sort="Zhou, Xiaomian" uniqKey="Zhou X" first="Xiaomian" last="Zhou">Xiaomian Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name></author><author><name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name></author><author><name sortKey="Dai, Zhongpeng" sort="Dai, Zhongpeng" uniqKey="Dai Z" first="Zhongpeng" last="Dai">Zhongpeng Dai</name></author><author><name sortKey="Wu, Dapeng" sort="Wu, Dapeng" uniqKey="Wu D" first="Dapeng" last="Wu">Dapeng Wu</name></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name></author><author><name sortKey="Du, Yuguang" sort="Du, Yuguang" uniqKey="Du Y" first="Yuguang" last="Du">Yuguang Du</name></author><author><name sortKey="Xia, Zhinan" sort="Xia, Zhinan" uniqKey="Xia Z" first="Zhinan" last="Xia">Zhinan Xia</name></author><author><name sortKey="Zhang, Liping" sort="Zhang, Liping" uniqKey="Zhang L" first="Liping" last="Zhang">Liping Zhang</name></author><author><name sortKey="Mei, Xiaodai" sort="Mei, Xiaodai" uniqKey="Mei X" first="Xiaodai" last="Mei">Xiaodai Mei</name></author><author><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name></author></analytic><series><title level="j">Electrophoresis</title><idno type="ISSN">0173-0835</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Autopsy</term><term>Electrophoresis, Capillary (methods)</term><term>Humans</term><term>Lab-On-A-Chip Devices</term><term>Lung (virology)</term><term>Microchip Analytical Procedures (methods)</term><term>Polymerase Chain Reaction</term><term>SARS Virus (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Autopsie</term><term>Humains</term><term>Laboratoires sur puces</term><term>Poumon (virologie)</term><term>Procédures d'analyse sur micropuce ()</term><term>Réaction de polymérisation en chaîne</term><term>Virus du SRAS (isolement et purification)</term><term>Électrophorèse capillaire ()</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term><term>Microchip Analytical Procedures</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Autopsy</term><term>Humans</term><term>Lab-On-A-Chip Devices</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Autopsie</term><term>Humains</term><term>Laboratoires sur puces</term><term>Procédures d'analyse sur micropuce</term><term>Réaction de polymérisation en chaîne</term><term>Électrophorèse capillaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-CoV). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15349945</PMID><DateCompleted><Year>2005</Year><Month>03</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0173-0835</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>17</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Electrophoresis</Title><ISOAbbreviation>Electrophoresis</ISOAbbreviation></Journal><ArticleTitle>Determination of SARS-coronavirus by a microfluidic chip system.</ArticleTitle><Pagination><MedlinePgn>3032-9</MedlinePgn></Pagination><Abstract><AbstractText>We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS-CoV). The system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS-CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT-PCR on SARS-CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home-made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS-CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT-PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Xiaomian</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. 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ValidYN="Y"><LastName>Lin</LastName><ForeName>Bingcheng</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Electrophoresis</MedlineTA><NlmUniqueID>8204476</NlmUniqueID><ISSNLinking>0173-0835</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001344" MajorTopicYN="N">Autopsy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019075" MajorTopicYN="N">Electrophoresis, Capillary</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056656" 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