Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.
Identifieur interne : 002B44 ( PubMed/Curation ); précédent : 002B43; suivant : 002B45Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.
Auteurs : Hai Pang [République populaire de Chine] ; Yinggang Liu [République populaire de Chine] ; Xueqing Han [République populaire de Chine] ; Yanhui Xu [République populaire de Chine] ; Fuguo Jiang [République populaire de Chine] ; Donglai Wu [République populaire de Chine] ; Xiangang Kong [République populaire de Chine] ; Mark Bartlam [République populaire de Chine] ; Zihe Rao [République populaire de Chine]Source :
- The Journal of general virology [ 0022-1317 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Protéines virales, Vaccins antiviraux, Virus du SRAS.
- sang : Anticorps antiviraux.
- Animaux, Cellules Vero, Lapins, Mâle, Syndrome respiratoire aigu sévère, Tests de neutralisation.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : SARS Virus, Viral Proteins, Viral Vaccines.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Male, Neutralization Tests, Rabbits, Vero Cells.
Abstract
Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.
DOI: 10.1099/vir.0.80111-0
PubMed: 15448374
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002B44
Links to Exploration step
pubmed:15448374Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.</title><author><name sortKey="Pang, Hai" sort="Pang, Hai" uniqKey="Pang H" first="Hai" last="Pang">Hai Pang</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Yinggang" sort="Liu, Yinggang" uniqKey="Liu Y" first="Yinggang" last="Liu">Yinggang Liu</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea></affiliation></author><author><name sortKey="Han, Xueqing" sort="Han, Xueqing" uniqKey="Han X" first="Xueqing" last="Han">Xueqing Han</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Fuguo" sort="Jiang, Fuguo" uniqKey="Jiang F" first="Fuguo" last="Jiang">Fuguo Jiang</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Donglai" sort="Wu, Donglai" uniqKey="Wu D" first="Donglai" last="Wu">Donglai Wu</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea></affiliation></author><author><name sortKey="Kong, Xiangang" sort="Kong, Xiangang" uniqKey="Kong X" first="Xiangang" last="Kong">Xiangang Kong</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea></affiliation></author><author><name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15448374</idno><idno type="pmid">15448374</idno><idno type="doi">10.1099/vir.0.80111-0</idno><idno type="wicri:Area/PubMed/Corpus">002B44</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B44</idno><idno type="wicri:Area/PubMed/Curation">002B44</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B44</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.</title><author><name sortKey="Pang, Hai" sort="Pang, Hai" uniqKey="Pang H" first="Hai" last="Pang">Hai Pang</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Yinggang" sort="Liu, Yinggang" uniqKey="Liu Y" first="Yinggang" last="Liu">Yinggang Liu</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea></affiliation></author><author><name sortKey="Han, Xueqing" sort="Han, Xueqing" uniqKey="Han X" first="Xueqing" last="Han">Xueqing Han</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Fuguo" sort="Jiang, Fuguo" uniqKey="Jiang F" first="Fuguo" last="Jiang">Fuguo Jiang</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Donglai" sort="Wu, Donglai" uniqKey="Wu D" first="Donglai" last="Wu">Donglai Wu</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea></affiliation></author><author><name sortKey="Kong, Xiangang" sort="Kong, Xiangang" uniqKey="Kong X" first="Xiangang" last="Kong">Xiangang Kong</name><affiliation wicri:level="1"><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001</wicri:regionArea></affiliation></author><author><name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing</wicri:regionArea></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Chlorocebus aethiops</term><term>Male</term><term>Neutralization Tests</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Vero Cells</term><term>Viral Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Cellules Vero</term><term>Lapins</term><term>Mâle</term><term>Protéines virales (immunologie)</term><term>Syndrome respiratoire aigu sévère ()</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines virales</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Viral Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Male</term><term>Neutralization Tests</term><term>Rabbits</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Lapins</term><term>Mâle</term><term>Syndrome respiratoire aigu sévère</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15448374</PMID><DateCompleted><Year>2004</Year><Month>10</Month><Day>21</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>85</Volume><Issue>Pt 10</Issue><PubDate><Year>2004</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.</ArticleTitle><Pagination><MedlinePgn>3109-3113</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.80111-0</ELocationID><Abstract><AbstractText>Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pang</LastName><ForeName>Hai</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yinggang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Xueqing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Yanhui</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Fuguo</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Donglai</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kong</LastName><ForeName>Xiangang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture, 427 Maduan Street, Harbin 150001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bartlam</LastName><ForeName>Mark</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rao</LastName><ForeName>Zihe</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009500" MajorTopicYN="N">Neutralization Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>9</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>10</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>9</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15448374</ArticleId><ArticleId IdType="doi">10.1099/vir.0.80111-0</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B44 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 002B44 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:15448374
|texte= Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:15448374" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |