High-throughput screening identifies inhibitors of the SARS coronavirus main proteinase.
Identifieur interne : 002B09 ( PubMed/Curation ); précédent : 002B08; suivant : 002B10High-throughput screening identifies inhibitors of the SARS coronavirus main proteinase.
Auteurs : Jan E. Blanchard [Canada] ; Nadine H. Elowe ; Carly Huitema ; Pascal D. Fortin ; Jonathan D. Cechetto ; Lindsay D. Eltis ; Eric D. BrownSource :
- Chemistry & biology [ 1074-5521 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (isolement et purification), Antiviraux (pharmacologie), Bovins, Cysteine endopeptidases, Endopeptidases (métabolisme), Inhibiteurs de protéases (), Inhibiteurs de protéases (isolement et purification), Inhibiteurs de protéases (pharmacologie), Protéines virales (antagonistes et inhibiteurs), Protéines virales (métabolisme), Spectrométrie de masse (), Virus du SRAS (), Virus du SRAS (enzymologie).
- MESH :
- antagonistes et inhibiteurs : Protéines virales.
- enzymologie : Virus du SRAS.
- isolement et purification : Antiviraux, Inhibiteurs de protéases.
- métabolisme : Endopeptidases, Protéines virales.
- pharmacologie : Antiviraux, Inhibiteurs de protéases.
- Animaux, Bovins, Cysteine endopeptidases, Inhibiteurs de protéases, Spectrométrie de masse, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (isolation & purification), Antiviral Agents (pharmacology), Cattle, Cysteine Endopeptidases, Endopeptidases (metabolism), Mass Spectrometry (methods), Protease Inhibitors (chemistry), Protease Inhibitors (isolation & purification), Protease Inhibitors (pharmacology), SARS Virus (drug effects), SARS Virus (enzymology), Viral Proteins (antagonists & inhibitors), Viral Proteins (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Viral Proteins.
- chemical , chemistry : Protease Inhibitors.
- chemical , isolation & purification : Antiviral Agents, Protease Inhibitors.
- chemical , metabolism : Endopeptidases, Viral Proteins.
- chemical , pharmacology : Antiviral Agents, Protease Inhibitors.
- drug effects : SARS Virus.
- enzymology : SARS Virus.
- methods : Mass Spectrometry.
- Animals, Cattle, Cysteine Endopeptidases.
Abstract
The causative agent of severe acute respiratory syndrome (SARS) has been identified as a novel coronavirus, SARS-CoV. The main proteinase of SARS-CoV, 3CLpro, is an attractive target for therapeutics against SARS owing to its fundamental role in viral replication. We sought to identify novel inhibitors of 3CLpro to advance the development of appropriate therapies in the treatment of SARS. 3CLpro was cloned, expressed, and purified from the Tor2 isolate. A quenched fluorescence resonance energy transfer assay was developed for 3CLpro to screen the proteinase against 50,000 drug-like small molecules on a fully automated system. The primary screen identified 572 hits; through a series of virtual and experimental filters, this number was reduced to five novel small molecules that show potent inhibitory activity (IC50 = 0.5-7 microM) toward SARS-CoV 3CLpro.
DOI: 10.1016/j.chembiol.2004.08.011
PubMed: 15489171
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pubmed:15489171Le document en format XML
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<front><div type="abstract" xml:lang="en">The causative agent of severe acute respiratory syndrome (SARS) has been identified as a novel coronavirus, SARS-CoV. The main proteinase of SARS-CoV, 3CLpro, is an attractive target for therapeutics against SARS owing to its fundamental role in viral replication. We sought to identify novel inhibitors of 3CLpro to advance the development of appropriate therapies in the treatment of SARS. 3CLpro was cloned, expressed, and purified from the Tor2 isolate. A quenched fluorescence resonance energy transfer assay was developed for 3CLpro to screen the proteinase against 50,000 drug-like small molecules on a fully automated system. The primary screen identified 572 hits; through a series of virtual and experimental filters, this number was reduced to five novel small molecules that show potent inhibitory activity (IC50 = 0.5-7 microM) toward SARS-CoV 3CLpro.</div>
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<Abstract><AbstractText>The causative agent of severe acute respiratory syndrome (SARS) has been identified as a novel coronavirus, SARS-CoV. The main proteinase of SARS-CoV, 3CLpro, is an attractive target for therapeutics against SARS owing to its fundamental role in viral replication. We sought to identify novel inhibitors of 3CLpro to advance the development of appropriate therapies in the treatment of SARS. 3CLpro was cloned, expressed, and purified from the Tor2 isolate. A quenched fluorescence resonance energy transfer assay was developed for 3CLpro to screen the proteinase against 50,000 drug-like small molecules on a fully automated system. The primary screen identified 572 hits; through a series of virtual and experimental filters, this number was reduced to five novel small molecules that show potent inhibitory activity (IC50 = 0.5-7 microM) toward SARS-CoV 3CLpro.</AbstractText>
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<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Jan 16;279(3):1637-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14561748</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Jun 4;279(23):24765-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15037623</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>JAMA. 2003 Oct 1;290(13):1695-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14519691</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2003 Sep;84(Pt 9):2305-2315</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12917450</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13190-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14585926</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Anal Biochem. 2003 Sep 1;320(1):1-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12895464</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2001 Jul;75(14):6676-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11413334</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochemistry. 2004 Apr 20;43(15):4568-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15078103</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biomol Screen. 1999;4(2):67-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10838414</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2002 Jul 26;295(4):958-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12127988</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Anal Biochem. 1999 Feb 15;267(2):331-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10036138</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 1994 May 5;369(6475):72-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8164744</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biochem Biophys Methods. 1996 Dec 30;33(3):135-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9029259</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Protein Eng. 1994 Sep;7(9):1145-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7831286</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Bacteriol. 1997 Jul;179(13):4403-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9209061</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Clin Infect Dis. 2003 Oct 15;37(8):1139-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14523782</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1986-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12682352</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Med Chem. 2002 Apr 11;45(8):1712-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11931626</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3156376</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochemistry. 1992 Apr 7;31(13):3358-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1313294</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10012-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15226499</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2000 Apr;81(Pt 4):853-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10725411</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Drug Deliv Rev. 2001 Mar 1;46(1-3):3-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11259830</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Med Chem. 1998 Jul 16;41(15):2806-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9667970</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Bioorg Med Chem Lett. 2003 Aug 4;13(15):2493-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12852950</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Chem Inf Comput Sci. 2004 May-Jun;44(3):1111-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15154780</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Mar 12;279(11):10222-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14701815</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 Jun 13;300(5626):1763-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12746549</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2003 May 15;423(6937):240</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12748632</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Respirology. 2004 Jun;9(2):173-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15182266</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2004 Apr;10(4):581-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15200845</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Bioorg Med Chem Lett. 2003 Nov 17;13(22):3989-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14592491</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Acta Pharmacol Sin. 2003 Jun;24(6):497-504</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12791174</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11000-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10500114</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2003 Aug 15;308(1):148-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12890493</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Med Chem. 2004 Feb 26;47(5):1079-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14971887</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Jun 4;318(3):719-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15144898</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Chem Rev. 1997 Feb 5;97(1):133-172</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11848867</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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