Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice.
Identifieur interne : 002A04 ( PubMed/Curation ); précédent : 002A03; suivant : 002A05Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice.
Auteurs : Alexander N. Zakhartchouk [Canada] ; Sathiyanarayanan Viswanathan [Canada] ; James B. Mahony [Canada] ; Jack Gauldie [Canada] ; Lorne A. Babiuk [Canada]Source :
- The Journal of general virology [ 0022-1317 ] ; 2005.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Adenoviridae (métabolisme), Animaux, Anticorps antiviraux (biosynthèse), Femelle, Lymphocytes T (immunologie), Masse moléculaire, Phosphorylation, Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines recombinantes (biosynthèse), Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Vaccination (), Vaccins antiviraux (biosynthèse), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (immunologie), Évaluation préclinique de médicament.
- MESH :
- biosynthèse : Anticorps antiviraux, Protéines nucléocapside, Protéines recombinantes, Vaccins antiviraux.
- génétique : Adenoviridae, Protéines nucléocapside, Vaccins antiviraux, Virus du SRAS.
- immunologie : Lymphocytes T, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- métabolisme : Adenoviridae.
- Animaux, Femelle, Masse moléculaire, Phosphorylation, Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère, Vaccination, Vecteurs génétiques, Évaluation préclinique de médicament.
English descriptors
- KwdEn :
- Adenoviridae (genetics), Adenoviridae (metabolism), Animals, Antibodies, Viral (biosynthesis), Drug Evaluation, Preclinical, Female, Genetic Vectors, Mice, Mice, Inbred C57BL, Molecular Weight, Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Phosphorylation, Recombinant Proteins (biosynthesis), SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), T-Lymphocytes (immunology), Vaccination (methods), Viral Vaccines (biosynthesis), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Viral, Nucleocapsid Proteins, Recombinant Proteins, Viral Vaccines.
- genetics : Adenoviridae, Nucleocapsid Proteins, SARS Virus, Viral Vaccines.
- chemical , immunology : Nucleocapsid Proteins, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Viral Vaccines.
- metabolism : Adenoviridae.
- methods : Vaccination.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Drug Evaluation, Preclinical, Female, Genetic Vectors, Mice, Mice, Inbred C57BL, Molecular Weight, Phosphorylation.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.
DOI: 10.1099/vir.0.80530-0
PubMed: 15604448
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(biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Phosphorylation</term><term>Recombinant Proteins (biosynthesis)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>T-Lymphocytes (immunology)</term><term>Vaccination (methods)</term><term>Viral Vaccines (biosynthesis)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adenoviridae (génétique)</term><term>Adenoviridae (métabolisme)</term><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Femelle</term><term>Lymphocytes T (immunologie)</term><term>Masse moléculaire</term><term>Phosphorylation</term><term>Protéines nucléocapside (biosynthèse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (biosynthèse)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Vaccination ()</term><term>Vaccins antiviraux (biosynthèse)</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term><term>Vecteurs génétiques</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Évaluation préclinique de médicament</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Protéines nucléocapside</term><term>Protéines 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xml:lang="en"><term>Adenoviridae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adenoviridae</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Drug Evaluation, Preclinical</term><term>Female</term><term>Genetic Vectors</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Molecular Weight</term><term>Phosphorylation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Masse moléculaire</term><term>Phosphorylation</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccination</term><term>Vecteurs génétiques</term><term>Évaluation préclinique de médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15604448</PMID><DateCompleted><Year>2005</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>86</Volume><Issue>Pt 1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice.</ArticleTitle><Pagination><MedlinePgn>211-215</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.80530-0</ELocationID><Abstract><AbstractText>Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zakhartchouk</LastName><ForeName>Alexander N</ForeName><Initials>AN</Initials><AffiliationInfo><Affiliation>Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Viswanathan</LastName><ForeName>Sathiyanarayanan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mahony</LastName><ForeName>James B</ForeName><Initials>JB</Initials><AffiliationInfo><Affiliation>St Joseph's Hospital, Hamilton, ON, Canada L8N 4A6.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada L8N 3Z5.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gauldie</LastName><ForeName>Jack</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada L8N 3Z5.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babiuk</LastName><ForeName>Lorne A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000256" MajorTopicYN="N">Adenoviridae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004353" MajorTopicYN="N">Drug Evaluation, Preclinical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010766" MajorTopicYN="N">Phosphorylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014611" MajorTopicYN="N">Vaccination</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>12</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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