Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.
Identifieur interne : 002969 ( PubMed/Curation ); précédent : 002968; suivant : 002970Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.
Auteurs : Zhijun Wang [États-Unis] ; Zhenghong Yuan ; Mitsuharu Matsumoto ; Ulrich R. Hengge ; Yung-Fu ChangSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules cultivées, Femelle, Lymphocytes T cytotoxiques (cytologie), Lymphocytes T cytotoxiques (immunologie), Production d'anticorps (immunologie), Prolifération cellulaire, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Vaccination, Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- cytologie : Lymphocytes T cytotoxiques.
- génétique : Vaccins à ADN, Virus du SRAS.
- immunologie : Lymphocytes T cytotoxiques, Production d'anticorps, Syndrome respiratoire aigu sévère, Vaccins à ADN, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules cultivées, Femelle, Prolifération cellulaire, Souris, Souris de lignée BALB C, Vaccination.
English descriptors
- KwdEn :
- Animals, Antibody Formation (immunology), Cell Proliferation, Cells, Cultured, Female, Mice, Mice, Inbred BALB C, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), T-Lymphocytes, Cytotoxic (cytology), T-Lymphocytes, Cytotoxic (immunology), Vaccination, Vaccines, DNA (genetics), Vaccines, DNA (immunology).
- MESH :
- chemical , genetics : Vaccines, DNA.
- cytology : T-Lymphocytes, Cytotoxic.
- genetics : SARS Virus.
- immunology : Antibody Formation, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic, Vaccines, DNA.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Cell Proliferation, Cells, Cultured, Female, Mice, Mice, Inbred BALB C, Vaccination.
Abstract
To analyze the immune responses of DNA vaccine encoded different gene fragments of severe acute respiratory syndrome coronavirus (SARS-Cov), SARS-Cov gene fragments of membrane (M), nucleocapsid (N), spike a (Sa), and spike b (Sb) proteins were cloned into pcDNA3.1 (Invitrogen) vector to form plasmids pcDNAM, pcDNAN, pcDNASa, and pcDNASb, respectively. After mice were immunized intramuscularly with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmid, blood was collected and serum was separated. Humoral immune response was detected with the enzyme-linked immunosorbent assay, and cellular immune response of SARS-Cov DNA vaccines was detected with lymphoproliferation assay and cytotoxic T lymphocyte assay. Results show that cellular and humoral immune responses can be detected after immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmids in BALB/c mice. However, pcDNAM stimulated the highest cellular immune response than other plasmids, and pcDNASa-pcDNASb stimulated the highest humoral immune response in week 12. The present results not only suggest that DNA immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb could be used as potential DNA vaccination approaches to induce antibody in BALB/c mice, but also to illustrate that gene immunization with these SARS DNA vaccines different immune response characters.
DOI: 10.1016/j.bbrc.2004.11.147
PubMed: 15629440
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Hengge</name></author><author><name sortKey="Chang, Yung Fu" sort="Chang, Yung Fu" uniqKey="Chang Y" first="Yung-Fu" last="Chang">Yung-Fu Chang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15629440</idno><idno type="pmid">15629440</idno><idno type="doi">10.1016/j.bbrc.2004.11.147</idno><idno type="wicri:Area/PubMed/Corpus">002969</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002969</idno><idno type="wicri:Area/PubMed/Curation">002969</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002969</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.</title><author><name sortKey="Wang, Zhijun" sort="Wang, Zhijun" uniqKey="Wang Z" first="Zhijun" last="Wang">Zhijun Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. zw37@cornell.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, Zhenghong" sort="Yuan, Zhenghong" uniqKey="Yuan Z" first="Zhenghong" last="Yuan">Zhenghong Yuan</name></author><author><name sortKey="Matsumoto, Mitsuharu" sort="Matsumoto, Mitsuharu" uniqKey="Matsumoto M" first="Mitsuharu" last="Matsumoto">Mitsuharu Matsumoto</name></author><author><name sortKey="Hengge, Ulrich R" sort="Hengge, Ulrich R" uniqKey="Hengge U" first="Ulrich R" last="Hengge">Ulrich R. Hengge</name></author><author><name sortKey="Chang, Yung Fu" sort="Chang, Yung Fu" uniqKey="Chang Y" first="Yung-Fu" last="Chang">Yung-Fu Chang</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibody Formation (immunology)</term><term>Cell Proliferation</term><term>Cells, Cultured</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>T-Lymphocytes, Cytotoxic (cytology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Vaccination</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Lymphocytes T cytotoxiques (cytologie)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Production d'anticorps (immunologie)</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Vaccination</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term><term>Production d'anticorps</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Formation</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes, Cytotoxic</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Proliferation</term><term>Cells, Cultured</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To analyze the immune responses of DNA vaccine encoded different gene fragments of severe acute respiratory syndrome coronavirus (SARS-Cov), SARS-Cov gene fragments of membrane (M), nucleocapsid (N), spike a (Sa), and spike b (Sb) proteins were cloned into pcDNA3.1 (Invitrogen) vector to form plasmids pcDNAM, pcDNAN, pcDNASa, and pcDNASb, respectively. After mice were immunized intramuscularly with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmid, blood was collected and serum was separated. Humoral immune response was detected with the enzyme-linked immunosorbent assay, and cellular immune response of SARS-Cov DNA vaccines was detected with lymphoproliferation assay and cytotoxic T lymphocyte assay. Results show that cellular and humoral immune responses can be detected after immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmids in BALB/c mice. However, pcDNAM stimulated the highest cellular immune response than other plasmids, and pcDNASa-pcDNASb stimulated the highest humoral immune response in week 12. The present results not only suggest that DNA immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb could be used as potential DNA vaccination approaches to induce antibody in BALB/c mice, but also to illustrate that gene immunization with these SARS DNA vaccines different immune response characters.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15629440</PMID><DateCompleted><Year>2005</Year><Month>03</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>327</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Feb</Month><Day>04</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem. Biophys. Res. Commun.</ISOAbbreviation></Journal><ArticleTitle>Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.</ArticleTitle><Pagination><MedlinePgn>130-5</MedlinePgn></Pagination><Abstract><AbstractText>To analyze the immune responses of DNA vaccine encoded different gene fragments of severe acute respiratory syndrome coronavirus (SARS-Cov), SARS-Cov gene fragments of membrane (M), nucleocapsid (N), spike a (Sa), and spike b (Sb) proteins were cloned into pcDNA3.1 (Invitrogen) vector to form plasmids pcDNAM, pcDNAN, pcDNASa, and pcDNASb, respectively. After mice were immunized intramuscularly with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmid, blood was collected and serum was separated. Humoral immune response was detected with the enzyme-linked immunosorbent assay, and cellular immune response of SARS-Cov DNA vaccines was detected with lymphoproliferation assay and cytotoxic T lymphocyte assay. Results show that cellular and humoral immune responses can be detected after immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmids in BALB/c mice. However, pcDNAM stimulated the highest cellular immune response than other plasmids, and pcDNASa-pcDNASb stimulated the highest humoral immune response in week 12. The present results not only suggest that DNA immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb could be used as potential DNA vaccination approaches to induce antibody in BALB/c mice, but also to illustrate that gene immunization with these SARS DNA vaccines different immune response characters.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhijun</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. zw37@cornell.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Zhenghong</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Matsumoto</LastName><ForeName>Mitsuharu</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hengge</LastName><ForeName>Ulrich R</ForeName><Initials>UR</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Yung-Fu</ForeName><Initials>YF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000917" MajorTopicYN="N">Antibody Formation</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell 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