SrasV1, PubMed, Curation, bibRecord, 002813

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.

Identifieur interne : 002813 ( PubMed/Curation ); précédent : 002812; suivant : 002814

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.

Auteurs : W C Yam [République populaire de Chine] ; K H Chan ; K H Chow ; L L M. Poon ; H Y Lam ; K Y Yuen ; W H Seto ; J S M. Peiris

Source :

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [ 1386-6532 ] ; 2005.

RBID : pubmed:15797361

Descripteurs français

KwdFr :
- Facteurs temps, Flambées de maladies, Humains, RT-PCR, Réaction de polymérisation en chaîne (), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Trousses de réactifs pour diagnostic, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Facteurs temps, Flambées de maladies, Humains, RT-PCR, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Trousses de réactifs pour diagnostic.

English descriptors

KwdEn :
- Disease Outbreaks, Humans, Polymerase Chain Reaction (methods), Reagent Kits, Diagnostic, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Time Factors.
MESH :
- chemical : Reagent Kits, Diagnostic.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Disease Outbreaks, Humans, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Time Factors.

Abstract

The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease.

DOI: 10.1016/j.jcv.2004.09.029
PubMed: 15797361

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pubmed:15797361

Le document en format XML

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<author><name sortKey="Yam, W C" sort="Yam, W C" uniqKey="Yam W" first="W C" last="Yam">W C Yam</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong SAR, PR China. wcyam@hku.hk</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong SAR</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chan, K H" sort="Chan, K H" uniqKey="Chan K" first="K H" last="Chan">K H Chan</name>
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<author><name sortKey="Chow, K H" sort="Chow, K H" uniqKey="Chow K" first="K H" last="Chow">K H Chow</name>
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<author><name sortKey="Poon, L L M" sort="Poon, L L M" uniqKey="Poon L" first="L L M" last="Poon">L L M. Poon</name>
</author>
<author><name sortKey="Lam, H Y" sort="Lam, H Y" uniqKey="Lam H" first="H Y" last="Lam">H Y Lam</name>
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<author><name sortKey="Yuen, K Y" sort="Yuen, K Y" uniqKey="Yuen K" first="K Y" last="Yuen">K Y Yuen</name>
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<author><name sortKey="Seto, W H" sort="Seto, W H" uniqKey="Seto W" first="W H" last="Seto">W H Seto</name>
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<author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name>
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<term>Humans</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Reagent Kits, Diagnostic</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (epidemiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs temps</term>
<term>Flambées de maladies</term>
<term>Humains</term>
<term>RT-PCR</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Sensibilité et spécificité</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Syndrome respiratoire aigu sévère (épidémiologie)</term>
<term>Trousses de réactifs pour diagnostic</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Reagent Kits, Diagnostic</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
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<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term>
<term>Humans</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
<term>Time Factors</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term>
<term>Flambées de maladies</term>
<term>Humains</term>
<term>RT-PCR</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Trousses de réactifs pour diagnostic</term>
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<front><div type="abstract" xml:lang="en">The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15797361</PMID>
<DateCompleted><Year>2005</Year>
<Month>07</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1386-6532</ISSN>
<JournalIssue CitedMedium="Print"><Volume>33</Volume>
<Issue>1</Issue>
<PubDate><Year>2005</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</Title>
<ISOAbbreviation>J. Clin. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.</ArticleTitle>
<Pagination><MedlinePgn>19-24</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease.</AbstractText>
<AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong.</AbstractText>
<AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n = 44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yam</LastName>
<ForeName>W C</ForeName>
<Initials>WC</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong SAR, PR China. wcyam@hku.hk</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>K H</ForeName>
<Initials>KH</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chow</LastName>
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<Author ValidYN="Y"><LastName>Poon</LastName>
<ForeName>L L M</ForeName>
<Initials>LL</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lam</LastName>
<ForeName>H Y</ForeName>
<Initials>HY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yuen</LastName>
<ForeName>K Y</ForeName>
<Initials>KY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Seto</LastName>
<ForeName>W H</ForeName>
<Initials>WH</Initials>
</Author>
<Author ValidYN="Y"><LastName>Peiris</LastName>
<ForeName>J S M</ForeName>
<Initials>JS</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
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<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>J Clin Virol</MedlineTA>
<NlmUniqueID>9815671</NlmUniqueID>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D004196" MajorTopicYN="Y">Disease Outbreaks</DescriptorName>
</MeshHeading>
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</MeshHeading>
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<MeshHeading><DescriptorName UI="D011933" MajorTopicYN="Y">Reagent Kits, Diagnostic</DescriptorName>
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<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
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<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
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<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
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<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
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Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods.

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Abstract

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