[Severe acute respiratory syndrome. The public health laboratory in a global emergency].
Identifieur interne : 002792 ( PubMed/Curation ); précédent : 002791; suivant : 002793[Severe acute respiratory syndrome. The public health laboratory in a global emergency].
Auteurs : Elsa G. Baumeister [Argentine] ; Adrián P. Lewis ; Juan P. Bozzini ; Vilma L. SavySource :
- Medicina [ 0025-7680 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- isolement et purification : Virus du SRAS.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Flambées de maladies, Humains, Réaction de polymérisation en chaîne, Santé mondiale, Techniques de laboratoire clinique, Urgences.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- Clinical Laboratory Techniques, Disease Outbreaks, Emergencies, Global Health, Humans.
Abstract
By the end of year 2002 there was an outbreak of atypical pneumonia in Southeast Asia which soon spread to other continents. This new severe acute respiratory syndrome (SARS) was produced by a novel coronavirus. Due to the severity of the situation and risk of introduction of this pathology in our country, the need to arrange specific laboratory diagnostic tests arose. Classic techniques, such as the electron microscopy and molecular biology test such as retrotranscription followed by the polymerase chain reaction (RT-PCR) were implemented. The araldit included cells infected with bovine coronavirus which allowed the viral particles to be visualized easily but it took more time in comparison with the negative staining of free particles from viral cultures. RT-PCR was able to detect RNA of isolated viruses from cases in Hong Kong and Germany.
PubMed: 15830791
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This new severe acute respiratory syndrome (SARS) was produced by a novel coronavirus. Due to the severity of the situation and risk of introduction of this pathology in our country, the need to arrange specific laboratory diagnostic tests arose. Classic techniques, such as the electron microscopy and molecular biology test such as retrotranscription followed by the polymerase chain reaction (RT-PCR) were implemented. The araldit included cells infected with bovine coronavirus which allowed the viral particles to be visualized easily but it took more time in comparison with the negative staining of free particles from viral cultures. RT-PCR was able to detect RNA of isolated viruses from cases in Hong Kong and Germany.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15830791</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0025-7680</ISSN><JournalIssue CitedMedium="Print"><Volume>65</Volume><Issue>1</Issue><PubDate><Year>2005</Year></PubDate></JournalIssue><Title>Medicina</Title><ISOAbbreviation>Medicina (B Aires)</ISOAbbreviation></Journal><ArticleTitle>[Severe acute respiratory syndrome. The public health laboratory in a global emergency].</ArticleTitle><Pagination><MedlinePgn>36-40</MedlinePgn></Pagination><Abstract><AbstractText>By the end of year 2002 there was an outbreak of atypical pneumonia in Southeast Asia which soon spread to other continents. This new severe acute respiratory syndrome (SARS) was produced by a novel coronavirus. Due to the severity of the situation and risk of introduction of this pathology in our country, the need to arrange specific laboratory diagnostic tests arose. Classic techniques, such as the electron microscopy and molecular biology test such as retrotranscription followed by the polymerase chain reaction (RT-PCR) were implemented. The araldit included cells infected with bovine coronavirus which allowed the viral particles to be visualized easily but it took more time in comparison with the negative staining of free particles from viral cultures. RT-PCR was able to detect RNA of isolated viruses from cases in Hong Kong and Germany.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Baumeister</LastName><ForeName>Elsa G</ForeName><Initials>EG</Initials><AffiliationInfo><Affiliation>Servicio de Virosis Respiratorias, Departamento de Virología, Instituto Nacional de Enfermedades Infecciosas, ANLIS Malbrán, Buenos Aires, Argentina. ebaumeister@anlis.gov.ar</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lewis</LastName><ForeName>Adrián P</ForeName><Initials>AP</Initials></Author><Author ValidYN="Y"><LastName>Bozzini</LastName><ForeName>Juan P</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Savy</LastName><ForeName>Vilma L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>spa</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><VernacularTitle>Síndrome respiratorio agudo grave. 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