Expression, purification, and characterization of SARS coronavirus RNA polymerase.
Identifieur interne : 002780 ( PubMed/Curation ); précédent : 002779; suivant : 002781Expression, purification, and characterization of SARS coronavirus RNA polymerase.
Auteurs : Ao Cheng [République populaire de Chine] ; Wei Zhang ; Youhua Xie ; Weihong Jiang ; Eddy Arnold ; Stefan G. Sarafianos ; Jianping DingSource :
- Virology [ 0042-6822 ] ; 2005.
Descripteurs français
- KwdFr :
- Agents antiVIH (pharmacologie), Clonage moléculaire, Domaine catalytique, Escherichia coli (génétique), Expression des gènes, Fragments peptidiques (), Fragments peptidiques (génétique), Fragments peptidiques (isolement et purification), Fragments peptidiques (métabolisme), Inhibiteurs de la transcriptase inverse (pharmacologie), Protéines recombinantes (), Protéines recombinantes (génétique), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), RNA replicase (), RNA replicase (génétique), RNA replicase (isolement et purification), RNA replicase (métabolisme), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (enzymologie).
- MESH :
- enzymologie : Virus du SRAS.
- génétique : Escherichia coli, Fragments peptidiques, Protéines recombinantes, RNA replicase.
- isolement et purification : Fragments peptidiques, Protéines recombinantes, RNA replicase.
- métabolisme : Fragments peptidiques, Protéines recombinantes, RNA replicase.
- pharmacologie : Agents antiVIH, Inhibiteurs de la transcriptase inverse.
- virologie : Syndrome respiratoire aigu sévère.
- Clonage moléculaire, Domaine catalytique, Expression des gènes, Fragments peptidiques, Protéines recombinantes, RNA replicase.
English descriptors
- KwdEn :
- Anti-HIV Agents (pharmacology), Catalytic Domain, Cloning, Molecular, Escherichia coli (genetics), Gene Expression, Peptide Fragments (chemistry), Peptide Fragments (genetics), Peptide Fragments (isolation & purification), Peptide Fragments (metabolism), RNA Replicase (chemistry), RNA Replicase (genetics), RNA Replicase (isolation & purification), RNA Replicase (metabolism), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Reverse Transcriptase Inhibitors (pharmacology), SARS Virus (enzymology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , chemistry : Peptide Fragments, RNA Replicase, Recombinant Proteins.
- chemical , genetics : Peptide Fragments, RNA Replicase, Recombinant Proteins.
- chemical , isolation & purification : Peptide Fragments, RNA Replicase, Recombinant Proteins.
- chemical , metabolism : Peptide Fragments, RNA Replicase, Recombinant Proteins.
- chemical , pharmacology : Anti-HIV Agents, Reverse Transcriptase Inhibitors.
- enzymology : SARS Virus.
- genetics : Escherichia coli.
- virology : Severe Acute Respiratory Syndrome.
- Catalytic Domain, Cloning, Molecular, Gene Expression.
Abstract
The RNA-dependent RNA polymerase (RdRp) of SARS coronavirus (SARS-CoV) is essential for viral replication and a potential target for anti-SARS drugs. We report here the cloning, expression, and purification of the N-terminal GST-fused SARS-CoV RdRp and its polymerase catalytic domain in Escherichia coli. During purification, the full-length GST-RdRp was found to cleave into three main fragments: an N-terminal p12 fragment, a middle p30 fragment, and a C-terminal p64 fragment comprising the catalytic domain, presumably due to bacterial proteases. Biochemical assays show that the full-length GST-RdRp has RdRp activity and the p64 and p12 fragments form a complex that exhibits comparable RdRp activity, whereas the GST-p64 protein has no activity, suggesting that the p12 domain is required for polymerase activity possibly via involvement in template-primer binding. Nonnucleoside HIV-1 RT inhibitors are shown to have no evident inhibitory effect on SARS-CoV RdRp activity. This work provides a basis for biochemical and structural studies of SARS-CoV RdRp and for development of anti-SARS drugs.
DOI: 10.1016/j.virol.2005.02.017
PubMed: 15840516
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We report here the cloning, expression, and purification of the N-terminal GST-fused SARS-CoV RdRp and its polymerase catalytic domain in Escherichia coli. During purification, the full-length GST-RdRp was found to cleave into three main fragments: an N-terminal p12 fragment, a middle p30 fragment, and a C-terminal p64 fragment comprising the catalytic domain, presumably due to bacterial proteases. Biochemical assays show that the full-length GST-RdRp has RdRp activity and the p64 and p12 fragments form a complex that exhibits comparable RdRp activity, whereas the GST-p64 protein has no activity, suggesting that the p12 domain is required for polymerase activity possibly via involvement in template-primer binding. Nonnucleoside HIV-1 RT inhibitors are shown to have no evident inhibitory effect on SARS-CoV RdRp activity. This work provides a basis for biochemical and structural studies of SARS-CoV RdRp and for development of anti-SARS drugs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15840516</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>335</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>May</Month><Day>10</Day></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Expression, purification, and characterization of SARS coronavirus RNA polymerase.</ArticleTitle><Pagination><MedlinePgn>165-76</MedlinePgn></Pagination><Abstract><AbstractText>The RNA-dependent RNA polymerase (RdRp) of SARS coronavirus (SARS-CoV) is essential for viral replication and a potential target for anti-SARS drugs. We report here the cloning, expression, and purification of the N-terminal GST-fused SARS-CoV RdRp and its polymerase catalytic domain in Escherichia coli. During purification, the full-length GST-RdRp was found to cleave into three main fragments: an N-terminal p12 fragment, a middle p30 fragment, and a C-terminal p64 fragment comprising the catalytic domain, presumably due to bacterial proteases. Biochemical assays show that the full-length GST-RdRp has RdRp activity and the p64 and p12 fragments form a complex that exhibits comparable RdRp activity, whereas the GST-p64 protein has no activity, suggesting that the p12 domain is required for polymerase activity possibly via involvement in template-primer binding. Nonnucleoside HIV-1 RT inhibitors are shown to have no evident inhibitory effect on SARS-CoV RdRp activity. This work provides a basis for biochemical and structural studies of SARS-CoV RdRp and for development of anti-SARS drugs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Ao</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Youhua</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Weihong</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Arnold</LastName><ForeName>Eddy</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Sarafianos</LastName><ForeName>Stefan 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IdType="pubmed">7542140</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell. 2002 Nov 27;111(5):733-45</Citation><ArticleIdList><ArticleId IdType="pubmed">12464184</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Sep;78(18):9977-86</Citation><ArticleIdList><ArticleId IdType="pubmed">15331731</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 1998 Jun 19;273(25):15479-86</Citation><ArticleIdList><ArticleId IdType="pubmed">9624134</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13190-5</Citation><ArticleIdList><ArticleId IdType="pubmed">14585926</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 1992 Jun 26;256(5065):1783-90</Citation><ArticleIdList><ArticleId IdType="pubmed">1377403</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2000 Jun;74(12):5647-54</Citation><ArticleIdList><ArticleId IdType="pubmed">10823872</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1977-85</Citation><ArticleIdList><ArticleId IdType="pubmed">12671062</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Med Chem. 2003 Apr 10;46(8):1283-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12672227</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1995 Jul;69(7):4331-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7769694</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2003 Oct;77(19):10515-27</Citation><ArticleIdList><ArticleId IdType="pubmed">12970436</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1999 Mar;73(3):2016-26</Citation><ArticleIdList><ArticleId IdType="pubmed">9971782</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2003 Jul;77(13):7575-81</Citation><ArticleIdList><ArticleId IdType="pubmed">12805457</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Viral Immunol. 2003;16(4):461-74</Citation><ArticleIdList><ArticleId IdType="pubmed">14733734</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation><ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Dec;78(24):13600-12</Citation><ArticleIdList><ArticleId IdType="pubmed">15564471</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1996 Dec 20;264(5):1085-100</Citation><ArticleIdList><ArticleId IdType="pubmed">9000632</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2003 Jul;77(13):7376-82</Citation><ArticleIdList><ArticleId IdType="pubmed">12805436</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1995-2005</Citation><ArticleIdList><ArticleId IdType="pubmed">12671061</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Struct Biol. 1995 Apr;2(4):303-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7540935</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2002 Apr;76(8):3697-708</Citation><ArticleIdList><ArticleId IdType="pubmed">11907209</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 1999 Mar;179(3):709-13</Citation><ArticleIdList><ArticleId IdType="pubmed">9952383</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 Jun 13;300(5626):1763-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12746549</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2002 Oct 11;277(41):38322-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12167642</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Rapid Commun Mass Spectrom. 2003;17(17):1995-2004</Citation><ArticleIdList><ArticleId IdType="pubmed">12913863</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2003 Sep;84(Pt 9):2305-2315</Citation><ArticleIdList><ArticleId IdType="pubmed">12917450</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 1998 Feb;53(2):141-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9620206</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 2003 Dec 15;31(24):7117-30</Citation><ArticleIdList><ArticleId IdType="pubmed">14654687</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antimicrob Agents Chemother. 1998 Dec;42(12):3123-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9835502</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2000 Apr;74(7):3379-87</Citation><ArticleIdList><ArticleId IdType="pubmed">10708455</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Struct Biol. 1995 Apr;2(4):293-302</Citation><ArticleIdList><ArticleId IdType="pubmed">7540934</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId 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