A human SARS-CoV neutralizing antibody against epitope on S2 protein.
Identifieur interne : 002700 ( PubMed/Curation ); précédent : 002699; suivant : 002701A human SARS-CoV neutralizing antibody against epitope on S2 protein.
Auteurs : Jinzhu Duan [République populaire de Chine] ; Xiyun Yan ; Xueming Guo ; Wuchun Cao ; Wei Han ; Cai Qi ; Jing Feng ; Dongling Yang ; Guangxia Gao ; Gang JinSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Antibodies, Epitopes, B-Lymphocyte, Viral Envelope Proteins.
- immunology : B-Lymphocytes, SARS Virus.
- methods : Epitope Mapping, Immunoassay, Neutralization Tests.
- Humans, Peptide Library.
Abstract
An immune antibody phage-display library was constructed from B cells of SARS convalescent patients. More than 80 clones were selected from the library by using the whole inactivated SARS-CoV virions as target. One human scFv, B1, was characterized extensively. The B1 recognized SARS pseudovirus in vivo and competed with SARS sera for binding to SARS-CoV with high affinity (equilibrium dissociation constant, K(d) = 105 nM). The B1 also has potent neutralizing activities against infection by pseudovirus expressing SARS-CoV S protein in vitro. Finally, we found that the B1 recognized an epitope on S2 protein, especially within amino acids 1023-1189 of S2 protein. This study not only first made a human neutralizing antibody, which recognized an epitope on S2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of SARS protein and SARS vaccine design.
DOI: 10.1016/j.bbrc.2005.05.089
PubMed: 15939399
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sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name></author><author><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name></author><author><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name></author><author><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia Gao</name></author><author><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15939399</idno><idno type="pmid">15939399</idno><idno type="doi">10.1016/j.bbrc.2005.05.089</idno><idno type="wicri:Area/PubMed/Corpus">002700</idno><idno 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sortKey="Guo, Xueming" sort="Guo, Xueming" uniqKey="Guo X" first="Xueming" last="Guo">Xueming Guo</name></author><author><name sortKey="Cao, Wuchun" sort="Cao, Wuchun" uniqKey="Cao W" first="Wuchun" last="Cao">Wuchun Cao</name></author><author><name sortKey="Han, Wei" sort="Han, Wei" uniqKey="Han W" first="Wei" last="Han">Wei Han</name></author><author><name sortKey="Qi, Cai" sort="Qi, Cai" uniqKey="Qi C" first="Cai" last="Qi">Cai Qi</name></author><author><name sortKey="Feng, Jing" sort="Feng, Jing" uniqKey="Feng J" first="Jing" last="Feng">Jing Feng</name></author><author><name sortKey="Yang, Dongling" sort="Yang, Dongling" uniqKey="Yang D" first="Dongling" last="Yang">Dongling Yang</name></author><author><name sortKey="Gao, Guangxia" sort="Gao, Guangxia" uniqKey="Gao G" first="Guangxia" last="Gao">Guangxia Gao</name></author><author><name sortKey="Jin, Gang" sort="Jin, Gang" uniqKey="Jin G" first="Gang" last="Jin">Gang Jin</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies (immunology)</term><term>B-Lymphocytes (immunology)</term><term>Epitope Mapping (methods)</term><term>Epitopes, B-Lymphocyte (immunology)</term><term>Humans</term><term>Immunoassay (methods)</term><term>Neutralization Tests (methods)</term><term>Peptide Library</term><term>SARS Virus (immunology)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps (immunologie)</term><term>Banque de peptides</term><term>Cartographie épitopique ()</term><term>Dosage immunologique ()</term><term>Déterminants antigéniques des lymphocytes B (immunologie)</term><term>Humains</term><term>Lymphocytes B (immunologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Tests de neutralisation ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Epitopes, B-Lymphocyte</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Déterminants antigéniques des lymphocytes B</term><term>Lymphocytes B</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term><term>Immunoassay</term><term>Neutralization Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Cartographie épitopique</term><term>Dosage immunologique</term><term>Humains</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An immune antibody phage-display library was constructed from B cells of SARS convalescent patients. More than 80 clones were selected from the library by using the whole inactivated SARS-CoV virions as target. One human scFv, B1, was characterized extensively. The B1 recognized SARS pseudovirus in vivo and competed with SARS sera for binding to SARS-CoV with high affinity (equilibrium dissociation constant, K(d) = 105 nM). The B1 also has potent neutralizing activities against infection by pseudovirus expressing SARS-CoV S protein in vitro. Finally, we found that the B1 recognized an epitope on S2 protein, especially within amino acids 1023-1189 of S2 protein. This study not only first made a human neutralizing antibody, which recognized an epitope on S2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of SARS protein and SARS vaccine design.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15939399</PMID><DateCompleted><Year>2005</Year><Month>08</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>31</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>333</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jul</Month><Day>22</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem. Biophys. Res. Commun.</ISOAbbreviation></Journal><ArticleTitle>A human SARS-CoV neutralizing antibody against epitope on S2 protein.</ArticleTitle><Pagination><MedlinePgn>186-93</MedlinePgn></Pagination><Abstract><AbstractText>An immune antibody phage-display library was constructed from B cells of SARS convalescent patients. More than 80 clones were selected from the library by using the whole inactivated SARS-CoV virions as target. One human scFv, B1, was characterized extensively. The B1 recognized SARS pseudovirus in vivo and competed with SARS sera for binding to SARS-CoV with high affinity (equilibrium dissociation constant, K(d) = 105 nM). The B1 also has potent neutralizing activities against infection by pseudovirus expressing SARS-CoV S protein in vitro. Finally, we found that the B1 recognized an epitope on S2 protein, especially within amino acids 1023-1189 of S2 protein. This study not only first made a human neutralizing antibody, which recognized an epitope on S2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of SARS protein and SARS vaccine design.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Jinzhu</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Microbial Resources, Institute of Microbiology, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Xiyun</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Xueming</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Wuchun</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Cai</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Jing</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Dongling</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Guangxia</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Gang</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>05</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>05</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>8</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15939399</ArticleId><ArticleId IdType="pii">S0006-291X(05)01089-2</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2005.05.089</ArticleId><ArticleId IdType="pmc">PMC7092839</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nature. 2003 Jul 10;424(6945):121-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12853923</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Invest. 2003 Jun;111(11):1605-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12782660</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Feb;79(3):1389-96</Citation><ArticleIdList><ArticleId IdType="pubmed">15650165</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Immunol. 2004 Nov;113(2):145-50</Citation><ArticleIdList><ArticleId IdType="pubmed">15451470</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Microbiol Infect. 2004 Jul;10(7):676-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15214887</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2004 Sep 15;173(6):4050-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15356154</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunotechnology. 1998 Jan;3(4):271-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9530560</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 Jul 31;349(5):508-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12890855</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Biotechnol. 2000 Jan;18(1):75-80</Citation><ArticleIdList><ArticleId IdType="pubmed">10625396</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2000 Feb 11;296(1):57-86</Citation><ArticleIdList><ArticleId IdType="pubmed">10656818</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Oct;78(19):10328-35</Citation><ArticleIdList><ArticleId IdType="pubmed">15367599</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Sep;78(17):9007-15</Citation><ArticleIdList><ArticleId IdType="pubmed">15308697</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antivir Ther. 2004 Apr;9(2):287-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15134191</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Eur J Clin Microbiol Infect Dis. 2005 Jan;24(1):44-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15616839</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Jan 23;313(4):938-47</Citation><ArticleIdList><ArticleId IdType="pubmed">14706633</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2004 Aug 15;326(1):140-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15262502</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Feb;79(3):1635-44</Citation><ArticleIdList><ArticleId IdType="pubmed">15650189</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Anal Chem. 2003 Nov 15;75(22):6119-23</Citation><ArticleIdList><ArticleId IdType="pubmed">14615990</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation><ArticleIdList><ArticleId IdType="pubmed">14670965</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(13):6938-45</Citation><ArticleIdList><ArticleId IdType="pubmed">15194770</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Sep 10;322(1):93-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15313178</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2000 May 25;271(1):182-96</Citation><ArticleIdList><ArticleId IdType="pubmed">10814583</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Med. 2004 Aug;10(8):871-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15247913</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Chem. 2004 Jun;50(6):1036-42</Citation><ArticleIdList><ArticleId IdType="pubmed">15044316</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4240-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15010527</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Mar;79(6):3289-96</Citation><ArticleIdList><ArticleId IdType="pubmed">15731223</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2536-41</Citation><ArticleIdList><ArticleId IdType="pubmed">14983044</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Scand J Immunol. 1992 Jun;35(6):643-50</Citation><ArticleIdList><ArticleId IdType="pubmed">1376487</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biomaterials. 1998 Mar;19(4-5):397-406</Citation><ArticleIdList><ArticleId IdType="pubmed">9677153</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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