Sequence analysis and structural prediction of the severe acute respiratory syndrome coronavirus nsp5.
Identifieur interne : 002657 ( PubMed/Curation ); précédent : 002656; suivant : 002658Sequence analysis and structural prediction of the severe acute respiratory syndrome coronavirus nsp5.
Auteurs : Jia-Hai Lu [République populaire de Chine] ; Ding-Mei Zhang ; Guo-Ling Wang ; Zhong-Min Guo ; Juan Li ; Bing-Yan Tan ; Li-Ping Ou-Yang ; Wen-Hua Ling ; Xin-Bing Yu ; Nan-Shan ZhongSource :
- Acta biochimica et biophysica Sinica [ 1672-9145 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Protéines virales non structurales.
- génétique : Plasmides, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Adulte d'âge moyen, Animaux, Cellules COS, Femelle, Humains, Protéines virales non structurales, RT-PCR, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, COS Cells, Chlorocebus aethiops, Female, Humans, Middle Aged, Plasmids (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), Severe Acute Respiratory Syndrome (virology), Viral Nonstructural Proteins (biosynthesis), Viral Nonstructural Proteins (chemistry).
- MESH :
- chemical , biosynthesis : Viral Nonstructural Proteins.
- chemical , chemistry : Viral Nonstructural Proteins.
- genetics : Plasmids, SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, COS Cells, Chlorocebus aethiops, Female, Humans, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs. Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5. In this study, bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5. Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP). The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins. The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This study provided the foundation for further exploration of the protein's biological function, and contributed to the search for anti-SARS-CoV drugs.
DOI: 10.1111/j.1745-7270.2005.00066.x
PubMed: 15999208
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first="Zhong-Min" last="Guo">Zhong-Min Guo</name></author><author><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name></author><author><name sortKey="Tan, Bing Yan" sort="Tan, Bing Yan" uniqKey="Tan B" first="Bing-Yan" last="Tan">Bing-Yan Tan</name></author><author><name sortKey="Ou Yang, Li Ping" sort="Ou Yang, Li Ping" uniqKey="Ou Yang L" first="Li-Ping" last="Ou-Yang">Li-Ping Ou-Yang</name></author><author><name sortKey="Ling, Wen Hua" sort="Ling, Wen Hua" uniqKey="Ling W" first="Wen-Hua" last="Ling">Wen-Hua Ling</name></author><author><name sortKey="Yu, Xin Bing" sort="Yu, Xin Bing" uniqKey="Yu X" first="Xin-Bing" last="Yu">Xin-Bing Yu</name></author><author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15999208</idno><idno 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510080</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Ding Mei" sort="Zhang, Ding Mei" uniqKey="Zhang D" first="Ding-Mei" last="Zhang">Ding-Mei Zhang</name></author><author><name sortKey="Wang, Guo Ling" sort="Wang, Guo Ling" uniqKey="Wang G" first="Guo-Ling" last="Wang">Guo-Ling Wang</name></author><author><name sortKey="Guo, Zhong Min" sort="Guo, Zhong Min" uniqKey="Guo Z" first="Zhong-Min" last="Guo">Zhong-Min Guo</name></author><author><name sortKey="Li, Juan" sort="Li, Juan" uniqKey="Li J" first="Juan" last="Li">Juan Li</name></author><author><name sortKey="Tan, Bing Yan" sort="Tan, Bing Yan" uniqKey="Tan B" first="Bing-Yan" last="Tan">Bing-Yan Tan</name></author><author><name sortKey="Ou Yang, Li Ping" sort="Ou Yang, Li Ping" uniqKey="Ou Yang L" first="Li-Ping" last="Ou-Yang">Li-Ping Ou-Yang</name></author><author><name sortKey="Ling, Wen Hua" sort="Ling, Wen Hua" uniqKey="Ling W" first="Wen-Hua" last="Ling">Wen-Hua Ling</name></author><author><name sortKey="Yu, Xin Bing" sort="Yu, Xin Bing" uniqKey="Yu X" first="Xin-Bing" last="Yu">Xin-Bing Yu</name></author><author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></author></analytic><series><title level="j">Acta biochimica et biophysica Sinica</title><idno type="ISSN">1672-9145</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Middle Aged</term><term>Plasmids (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Viral Nonstructural Proteins (biosynthesis)</term><term>Viral Nonstructural Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte d'âge moyen</term><term>Animaux</term><term>Cellules COS</term><term>Femelle</term><term>Humains</term><term>Plasmides (génétique)</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (biosynthèse)</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Plasmides</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Middle Aged</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte d'âge moyen</term><term>Animaux</term><term>Cellules COS</term><term>Femelle</term><term>Humains</term><term>Protéines virales non structurales</term><term>RT-PCR</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs. Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5. In this study, bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5. Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP). The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins. The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This study provided the foundation for further exploration of the protein's biological function, and contributed to the search for anti-SARS-CoV drugs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15999208</PMID><DateCompleted><Year>2005</Year><Month>09</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1672-9145</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>7</Issue><PubDate><Year>2005</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Acta biochimica et biophysica Sinica</Title><ISOAbbreviation>Acta Biochim. Biophys. Sin. (Shanghai)</ISOAbbreviation></Journal><ArticleTitle>Sequence analysis and structural prediction of the severe acute respiratory syndrome coronavirus nsp5.</ArticleTitle><Pagination><MedlinePgn>473-9</MedlinePgn></Pagination><Abstract><AbstractText>The non-structural proteins (nsp or replicase proteins) of coronaviruses are relatively conserved and can be effective targets for drugs. Few studies have been conducted into the function of the severe acute respiratory syndrome coronavirus (SARS-CoV) nsp5. In this study, bioinformatics methods were employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD strain nsp5. Sequencing and sequential comparison was performed to analyze the mutation trend of the polymerase nsp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool (BLASTN) and a protein-protein basic local alignment search tool (BLASTP). The results indicated that the nsp5 gene was steady during the epidemic process and the protein was homologous with other coronavirus nsp5 proteins. The protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This study provided the foundation for further exploration of the protein's biological function, and contributed to the search for anti-SARS-CoV drugs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Jia-Hai</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>The Public Health School of Sun Yat-Sen University, Guangzhou 510080, China. jiahailu@yahoo.com.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ding-Mei</ForeName><Initials>DM</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Guo-Ling</ForeName><Initials>GL</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Zhong-Min</ForeName><Initials>ZM</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Juan</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Bing-Yan</ForeName><Initials>BY</Initials></Author><Author ValidYN="Y"><LastName>Ou-Yang</LastName><ForeName>Li-Ping</ForeName><Initials>LP</Initials></Author><Author ValidYN="Y"><LastName>Ling</LastName><ForeName>Wen-Hua</ForeName><Initials>WH</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Xin-Bing</ForeName><Initials>XB</Initials></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Nan-Shan</ForeName><Initials>NS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Acta Biochim Biophys Sin (Shanghai)</MedlineTA><NlmUniqueID>101206716</NlmUniqueID><ISSNLinking>1672-9145</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019556" MajorTopicYN="N">COS Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>7</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>9</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>7</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15999208</ArticleId><ArticleId IdType="doi">10.1111/j.1745-7270.2005.00066.x</ArticleId><ArticleId IdType="pmc">PMC7110076</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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