Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum: comparison with results of other viral markers.
Identifieur interne : 002636 ( PubMed/Curation ); précédent : 002635; suivant : 002637Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum: comparison with results of other viral markers.
Auteurs : Yong-Hua Li [République populaire de Chine] ; Jie Li ; Xue-En Liu ; Ling Wang ; Tong Li ; Yi-Hua Zhou ; Hui ZhuangSource :
- Journal of virological methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux, Anticorps monoclonaux, Dosage immunologique (), Femelle, Humains, Mâle, Protéines nucléocapside (immunologie), Protéines nucléocapside (sang), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (sang), Virus du SRAS (immunologie).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Protéines nucléocapside, Virus du SRAS.
- sang : Protéines nucléocapside, Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Anticorps antiviraux, Anticorps monoclonaux, Dosage immunologique, Femelle, Humains, Mâle, Sensibilité et spécificité.
English descriptors
- KwdEn :
- Adult, Antibodies, Monoclonal, Antibodies, Viral, Female, Humans, Immunoassay (methods), Male, Middle Aged, Nucleocapsid Proteins (blood), Nucleocapsid Proteins (immunology), SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (diagnosis).
- MESH :
- chemical , blood : Nucleocapsid Proteins.
- chemical , immunology : Nucleocapsid Proteins.
- chemical : Antibodies, Monoclonal, Antibodies, Viral.
- blood : Severe Acute Respiratory Syndrome.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- methods : Immunoassay.
- Adult, Female, Humans, Male, Middle Aged, Sensitivity and Specificity.
Abstract
A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.
DOI: 10.1016/j.jviromet.2005.06.001
PubMed: 16024098
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sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name></author><author><name sortKey="Li, Tong" sort="Li, Tong" uniqKey="Li T" first="Tong" last="Li">Tong Li</name></author><author><name sortKey="Zhou, Yi Hua" sort="Zhou, Yi Hua" uniqKey="Zhou Y" first="Yi-Hua" last="Zhou">Yi-Hua Zhou</name></author><author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16024098</idno><idno type="pmid">16024098</idno><idno type="doi">10.1016/j.jviromet.2005.06.001</idno><idno type="wicri:Area/PubMed/Corpus">002636</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002636</idno><idno type="wicri:Area/PubMed/Curation">002636</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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Liu</name></author><author><name sortKey="Wang, Ling" sort="Wang, Ling" uniqKey="Wang L" first="Ling" last="Wang">Ling Wang</name></author><author><name sortKey="Li, Tong" sort="Li, Tong" uniqKey="Li T" first="Tong" last="Li">Tong Li</name></author><author><name sortKey="Zhou, Yi Hua" sort="Zhou, Yi Hua" uniqKey="Zhou Y" first="Yi-Hua" last="Zhou">Yi-Hua Zhou</name></author><author><name sortKey="Zhuang, Hui" sort="Zhuang, Hui" uniqKey="Zhuang H" first="Hui" last="Zhuang">Hui Zhuang</name></author></analytic><series><title level="j">Journal of virological methods</title><idno type="ISSN">0166-0934</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Female</term><term>Humans</term><term>Immunoassay (methods)</term><term>Male</term><term>Middle Aged</term><term>Nucleocapsid Proteins (blood)</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (blood)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Dosage immunologique ()</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines nucléocapside (sang)</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (sang)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Dosage immunologique</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16024098</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0166-0934</ISSN><JournalIssue CitedMedium="Print"><Volume>130</Volume><Issue>1-2</Issue><PubDate><Year>2005</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Journal of virological methods</Title><ISOAbbreviation>J. Virol. Methods</ISOAbbreviation></Journal><ArticleTitle>Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum: comparison with results of other viral markers.</ArticleTitle><Pagination><MedlinePgn>45-50</MedlinePgn></Pagination><Abstract><AbstractText>A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yong-Hua</ForeName><Initials>YH</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xue-En</ForeName><Initials>XE</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ling</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Tong</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yi-Hua</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Zhuang</LastName><ForeName>Hui</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>07</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Virol Methods</MedlineTA><NlmUniqueID>8005839</NlmUniqueID><ISSNLinking>0166-0934</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007118" MajorTopicYN="N">Immunoassay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" 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