Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate.
Identifieur interne : 001E18 ( PubMed/Curation ); précédent : 001E17; suivant : 001E19Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate.
Auteurs : Louay K. Hallak [États-Unis] ; Steven A. Kwilas ; Mark E. PeeplesSource :
- Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Protéines membranaires, Protéoglycanes à sulfate d'héparane, Virus du SRAS.
- Animaux, Cellules CHO, Cricetinae, Cricetulus, Humains.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Heparan Sulfate Proteoglycans, Membrane Proteins.
- metabolism : SARS Virus.
- Animals, CHO Cells, Cricetinae, Cricetulus, Humans.
Abstract
Glycosaminoglycans (GAGs), including heparan sulfate (HS), are expressed on the surface of nearly all cells, linked to transmembrane proteins. These GAGs are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. Here we describe the rationale and methods for analyzing GAG usage by one such virus, respiratory syncytial virus (RSV). The protocols presented allow the determination of which GAG(s) is employed by the virus, which GAG modification(s) is important, and whether the important GAG is on the cell or on the virus. We also discuss the finding that many viruses are selected for GAG usage during passage in culture and present a method for rapidly determining whether GAG usage is characteristic of a wild virus or is limited to laboratory-adapted virus.
DOI: 10.1007/978-1-59745-393-6_2
PubMed: 17502668
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001E18
Links to Exploration step
pubmed:17502668Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate.</title>
<author><name sortKey="Hallak, Louay K" sort="Hallak, Louay K" uniqKey="Hallak L" first="Louay K" last="Hallak">Louay K. Hallak</name>
<affiliation wicri:level="1"><nlm:affiliation>Center for Vaccines and Immunity, Columbus Children's Research Institute, OH, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Vaccines and Immunity, Columbus Children's Research Institute, OH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kwilas, Steven A" sort="Kwilas, Steven A" uniqKey="Kwilas S" first="Steven A" last="Kwilas">Steven A. Kwilas</name>
</author>
<author><name sortKey="Peeples, Mark E" sort="Peeples, Mark E" uniqKey="Peeples M" first="Mark E" last="Peeples">Mark E. Peeples</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2007">2007</date>
<idno type="RBID">pubmed:17502668</idno>
<idno type="pmid">17502668</idno>
<idno type="doi">10.1007/978-1-59745-393-6_2</idno>
<idno type="wicri:Area/PubMed/Corpus">001E18</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E18</idno>
<idno type="wicri:Area/PubMed/Curation">001E18</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E18</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate.</title>
<author><name sortKey="Hallak, Louay K" sort="Hallak, Louay K" uniqKey="Hallak L" first="Louay K" last="Hallak">Louay K. Hallak</name>
<affiliation wicri:level="1"><nlm:affiliation>Center for Vaccines and Immunity, Columbus Children's Research Institute, OH, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Center for Vaccines and Immunity, Columbus Children's Research Institute, OH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kwilas, Steven A" sort="Kwilas, Steven A" uniqKey="Kwilas S" first="Steven A" last="Kwilas">Steven A. Kwilas</name>
</author>
<author><name sortKey="Peeples, Mark E" sort="Peeples, Mark E" uniqKey="Peeples M" first="Mark E" last="Peeples">Mark E. Peeples</name>
</author>
</analytic>
<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
<idno type="ISSN">1064-3745</idno>
<imprint><date when="2007" type="published">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>CHO Cells</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Heparan Sulfate Proteoglycans (metabolism)</term>
<term>Humans</term>
<term>Membrane Proteins (metabolism)</term>
<term>SARS Virus (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules CHO</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Humains</term>
<term>Protéines membranaires (métabolisme)</term>
<term>Protéoglycanes à sulfate d'héparane (métabolisme)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Heparan Sulfate Proteoglycans</term>
<term>Membrane Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines membranaires</term>
<term>Protéoglycanes à sulfate d'héparane</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>CHO Cells</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules CHO</term>
<term>Cricetinae</term>
<term>Cricetulus</term>
<term>Humains</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Glycosaminoglycans (GAGs), including heparan sulfate (HS), are expressed on the surface of nearly all cells, linked to transmembrane proteins. These GAGs are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. Here we describe the rationale and methods for analyzing GAG usage by one such virus, respiratory syncytial virus (RSV). The protocols presented allow the determination of which GAG(s) is employed by the virus, which GAG modification(s) is important, and whether the important GAG is on the cell or on the virus. We also discuss the finding that many viruses are selected for GAG usage during passage in culture and present a method for rapidly determining whether GAG usage is characteristic of a wild virus or is limited to laboratory-adapted virus.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17502668</PMID>
<DateCompleted><Year>2007</Year>
<Month>06</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2007</Year>
<Month>12</Month>
<Day>03</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1064-3745</ISSN>
<JournalIssue CitedMedium="Print"><Volume>379</Volume>
<PubDate><Year>2007</Year>
</PubDate>
</JournalIssue>
<Title>Methods in molecular biology (Clifton, N.J.)</Title>
<ISOAbbreviation>Methods Mol. Biol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate.</ArticleTitle>
<Pagination><MedlinePgn>15-34</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Glycosaminoglycans (GAGs), including heparan sulfate (HS), are expressed on the surface of nearly all cells, linked to transmembrane proteins. These GAGs are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. Here we describe the rationale and methods for analyzing GAG usage by one such virus, respiratory syncytial virus (RSV). The protocols presented allow the determination of which GAG(s) is employed by the virus, which GAG modification(s) is important, and whether the important GAG is on the cell or on the virus. We also discuss the finding that many viruses are selected for GAG usage during passage in culture and present a method for rapidly determining whether GAG usage is characteristic of a wild virus or is limited to laboratory-adapted virus.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hallak</LastName>
<ForeName>Louay K</ForeName>
<Initials>LK</Initials>
<AffiliationInfo><Affiliation>Center for Vaccines and Immunity, Columbus Children's Research Institute, OH, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kwilas</LastName>
<ForeName>Steven A</ForeName>
<Initials>SA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Peeples</LastName>
<ForeName>Mark E</ForeName>
<Initials>ME</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>AI47213</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
<PublicationType UI="D016454">Review</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Methods Mol Biol</MedlineTA>
<NlmUniqueID>9214969</NlmUniqueID>
<ISSNLinking>1064-3745</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019812">Heparan Sulfate Proteoglycans</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008565">Membrane Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016466" MajorTopicYN="N">CHO Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003412" MajorTopicYN="N">Cricetulus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019812" MajorTopicYN="N">Heparan Sulfate Proteoglycans</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008565" MajorTopicYN="N">Membrane Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<NumberOfReferences>52</NumberOfReferences>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year>
<Month>5</Month>
<Day>16</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2007</Year>
<Month>6</Month>
<Day>15</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2007</Year>
<Month>5</Month>
<Day>16</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">17502668</ArticleId>
<ArticleId IdType="pii">1-59745-393-5:15</ArticleId>
<ArticleId IdType="doi">10.1007/978-1-59745-393-6_2</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E18 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001E18 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Curation |type= RBID |clé= pubmed:17502668 |texte= Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:17502668" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |