Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
Identifieur interne : 001539 ( PubMed/Curation ); précédent : 001538; suivant : 001540Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
Auteurs : Claire Debarnot [France] ; Isabelle Imbert ; François Ferron ; Laure Gluais ; Isabelle Varlet ; Nicolas Papageorgiou ; Mickaël Bouvet ; Julien Lescar ; Etienne Decroly ; Bruno CanardSource :
- Acta crystallographica. Section F, Structural biology and crystallization communications [ 1744-3091 ] ; 2011.
Descripteurs français
- KwdFr :
- Clonage moléculaire, Cristallisation, Cristallographie aux rayons X, Données de séquences moléculaires, Humains, Methyltransferases (), Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (isolement et purification), RNA replicase (), RNA replicase (génétique), RNA replicase (isolement et purification), Virus du SRAS ().
- MESH :
- génétique : Protéines virales non structurales, RNA replicase.
- isolement et purification : Protéines virales non structurales, RNA replicase.
- Clonage moléculaire, Cristallisation, Cristallographie aux rayons X, Données de séquences moléculaires, Humains, Methyltransferases, Protéines virales non structurales, RNA replicase, Virus du SRAS.
English descriptors
- KwdEn :
- Cloning, Molecular, Crystallization, Crystallography, X-Ray, Humans, Methyltransferases (chemistry), Molecular Sequence Data, RNA Replicase (chemistry), RNA Replicase (genetics), RNA Replicase (isolation & purification), SARS Virus (chemistry), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (isolation & purification).
- MESH :
- chemical , chemistry : Methyltransferases, RNA Replicase, Viral Nonstructural Proteins.
- chemical , genetics : RNA Replicase, Viral Nonstructural Proteins.
- chemical , isolation & purification : RNA Replicase, Viral Nonstructural Proteins.
- chemistry : SARS Virus.
- Cloning, Molecular, Crystallization, Crystallography, X-Ray, Humans, Molecular Sequence Data.
Abstract
To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.
DOI: 10.1107/S1744309111002867
PubMed: 21393853
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Section F, Structural biology and crystallization communications</title><idno type="eISSN">1744-3091</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Humans</term><term>Methyltransferases (chemistry)</term><term>Molecular Sequence Data</term><term>RNA Replicase (chemistry)</term><term>RNA Replicase (genetics)</term><term>RNA Replicase (isolation & purification)</term><term>SARS Virus (chemistry)</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Clonage moléculaire</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Methyltransferases ()</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (isolement et purification)</term><term>RNA replicase ()</term><term>RNA replicase (génétique)</term><term>RNA replicase (isolement et purification)</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Methyltransferases</term><term>RNA Replicase</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA Replicase</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA Replicase</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines virales non structurales</term><term>RNA replicase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Methyltransferases</term><term>Protéines virales non structurales</term><term>RNA replicase</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21393853</PMID><DateCompleted><Year>2011</Year><Month>06</Month><Day>27</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1744-3091</ISSN><JournalIssue CitedMedium="Internet"><Volume>67</Volume><Issue>Pt 3</Issue><PubDate><Year>2011</Year><Month>Mar</Month><Day>01</Day></PubDate></JournalIssue><Title>Acta crystallographica. Section F, Structural biology and crystallization communications</Title><ISOAbbreviation>Acta Crystallogr. Sect. F Struct. Biol. Cryst. 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The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Debarnot</LastName><ForeName>Claire</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Département de Virologie Structurale, Architecture et Fonction des Macromolécules Biologiques, UMR 6098, 163 Avenue de Luminy, 13288 Marseille CEDEX 09, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Imbert</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Ferron</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Gluais</LastName><ForeName>Laure</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Varlet</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Papageorgiou</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Bouvet</LastName><ForeName>Mickaël</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Lescar</LastName><ForeName>Julien</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Decroly</LastName><ForeName>Etienne</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Canard</LastName><ForeName>Bruno</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>02</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Acta Crystallogr Sect F Struct Biol Cryst 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IdType="pubmed">19052085</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3792-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15007178</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11892-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16882730</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2005 Nov;12(11):980-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16228002</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Aug;80(16):7902-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16873247</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2008 May;133(2):136-48</Citation><ArticleIdList><ArticleId IdType="pubmed">18255185</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2008 Aug;82(16):8071-84</Citation><ArticleIdList><ArticleId IdType="pubmed">18417574</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2010 Jan;91(Pt 1):112-21</Citation><ArticleIdList><ArticleId IdType="pubmed">19776234</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jun;81(12):6700-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17409150</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Nov;81(21):12049-60</Citation><ArticleIdList><ArticleId IdType="pubmed">17728234</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2003 Jun 13;113(6):701-2</Citation><ArticleIdList><ArticleId IdType="pubmed">12809601</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2010 Oct 22;285(43):33230-41</Citation><ArticleIdList><ArticleId IdType="pubmed">20699222</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1981 May;38(2):661-70</Citation><ArticleIdList><ArticleId IdType="pubmed">6165837</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2003 Sep;84(Pt 9):2305-15</Citation><ArticleIdList><ArticleId IdType="pubmed">12917450</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3</Citation><ArticleIdList><ArticleId IdType="pubmed">15299374</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Sep;80(17):8493-502</Citation><ArticleIdList><ArticleId IdType="pubmed">16912299</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2006 Oct 18;25(20):4933-42</Citation><ArticleIdList><ArticleId IdType="pubmed">17024178</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2002 Dec 2;21(23):6571-80</Citation><ArticleIdList><ArticleId IdType="pubmed">12456663</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 Apr;6(4):e1000863</Citation><ArticleIdList><ArticleId IdType="pubmed">20421945</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32</Citation><ArticleIdList><ArticleId IdType="pubmed">20124692</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2002 Jul 1;21(13):3213-24</Citation><ArticleIdList><ArticleId IdType="pubmed">12093723</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5717-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16581910</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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