Yeast based small molecule screen for inhibitors of SARS-CoV.
Identifieur interne : 001433 ( PubMed/Curation ); précédent : 001432; suivant : 001434Yeast based small molecule screen for inhibitors of SARS-CoV.
Auteurs : Matthew Frieman [États-Unis] ; Dipanwita Basu ; Krystal Matthews ; Justin Taylor ; Grant Jones ; Raymond Pickles ; Ralph Baric ; Daniel A. EngelSource :
- PloS one [ 1932-6203 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (pharmacologie), Cellules HEK293, Cellules Vero, Cellules épithéliales (cytologie), Clonage moléculaire, Conception de médicament, Humains, Liaison aux protéines, Milieux de culture (métabolisme), Orthomyxoviridae (génétique), Phénotype, Protéines à fluorescence verte (métabolisme), Saccharomyces cerevisiae (métabolisme), Saccharomyces cerevisiae (virologie), Techniques in vitro, Technologie pharmaceutique (), Trachée (métabolisme), Virus du SRAS (métabolisme).
- MESH :
- cytologie : Cellules épithéliales.
- génétique : Orthomyxoviridae.
- métabolisme : Milieux de culture, Protéines à fluorescence verte, Saccharomyces cerevisiae, Trachée, Virus du SRAS.
- pharmacologie : Antiviraux.
- virologie : Saccharomyces cerevisiae.
- Animaux, Cellules HEK293, Cellules Vero, Clonage moléculaire, Conception de médicament, Humains, Liaison aux protéines, Phénotype, Techniques in vitro, Technologie pharmaceutique.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (pharmacology), Chlorocebus aethiops, Cloning, Molecular, Culture Media (metabolism), Drug Design, Epithelial Cells (cytology), Green Fluorescent Proteins (metabolism), HEK293 Cells, Humans, In Vitro Techniques, Orthomyxoviridae (genetics), Phenotype, Protein Binding, SARS Virus (metabolism), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae (virology), Technology, Pharmaceutical (methods), Trachea (metabolism), Vero Cells.
- MESH :
- chemical , metabolism : Culture Media, Green Fluorescent Proteins.
- chemical , pharmacology : Antiviral Agents.
- cytology : Epithelial Cells.
- genetics : Orthomyxoviridae.
- metabolism : SARS Virus, Saccharomyces cerevisiae, Trachea.
- methods : Technology, Pharmaceutical.
- virology : Saccharomyces cerevisiae.
- Animals, Chlorocebus aethiops, Cloning, Molecular, Drug Design, HEK293 Cells, Humans, In Vitro Techniques, Phenotype, Protein Binding, Vero Cells.
Abstract
Severe acute respiratory coronavirus (SARS-CoV) emerged in 2002, resulting in roughly 8000 cases worldwide and 10% mortality. The animal reservoirs for SARS-CoV precursors still exist and the likelihood of future outbreaks in the human population is high. The SARS-CoV papain-like protease (PLP) is an attractive target for pharmaceutical development because it is essential for virus replication and is conserved among human coronaviruses. A yeast-based assay was established for PLP activity that relies on the ability of PLP to induce a pronounced slow-growth phenotype when expressed in S. cerevisiae. Induction of the slow-growth phenotype was shown to take place over a 60-hour time course, providing the basis for conducting a screen for small molecules that restore growth by inhibiting the function of PLP. Five chemical suppressors of the slow-growth phenotype were identified from the 2000 member NIH Diversity Set library. One of these, NSC158362, potently inhibited SARS-CoV replication in cell culture without toxic effects on cells, and it specifically inhibited SARS-CoV replication but not influenza virus replication. The effect of NSC158362 on PLP protease, deubiquitinase and anti-interferon activities was investigated but the compound did not alter these activities. Another suppressor, NSC158011, demonstrated the ability to inhibit PLP protease activity in a cell-based assay. The identification of these inhibitors demonstrated a strong functional connection between the PLP-based yeast assay, the inhibitory compounds, and SARS-CoV biology. Furthermore the data with NSC158362 suggest a novel mechanism for inhibition of SARS-CoV replication that may involve an unknown activity of PLP, or alternatively a direct effect on a cellular target that modifies or bypasses PLP function in yeast and mammalian cells.
DOI: 10.1371/journal.pone.0028479
PubMed: 22164298
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001433
Links to Exploration step
pubmed:22164298Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Yeast based small molecule screen for inhibitors of SARS-CoV.</title>
<author><name sortKey="Frieman, Matthew" sort="Frieman, Matthew" uniqKey="Frieman M" first="Matthew" last="Frieman">Matthew Frieman</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Basu, Dipanwita" sort="Basu, Dipanwita" uniqKey="Basu D" first="Dipanwita" last="Basu">Dipanwita Basu</name>
</author>
<author><name sortKey="Matthews, Krystal" sort="Matthews, Krystal" uniqKey="Matthews K" first="Krystal" last="Matthews">Krystal Matthews</name>
</author>
<author><name sortKey="Taylor, Justin" sort="Taylor, Justin" uniqKey="Taylor J" first="Justin" last="Taylor">Justin Taylor</name>
</author>
<author><name sortKey="Jones, Grant" sort="Jones, Grant" uniqKey="Jones G" first="Grant" last="Jones">Grant Jones</name>
</author>
<author><name sortKey="Pickles, Raymond" sort="Pickles, Raymond" uniqKey="Pickles R" first="Raymond" last="Pickles">Raymond Pickles</name>
</author>
<author><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name>
</author>
<author><name sortKey="Engel, Daniel A" sort="Engel, Daniel A" uniqKey="Engel D" first="Daniel A" last="Engel">Daniel A. Engel</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2011">2011</date>
<idno type="RBID">pubmed:22164298</idno>
<idno type="pmid">22164298</idno>
<idno type="doi">10.1371/journal.pone.0028479</idno>
<idno type="wicri:Area/PubMed/Corpus">001433</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001433</idno>
<idno type="wicri:Area/PubMed/Curation">001433</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001433</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Yeast based small molecule screen for inhibitors of SARS-CoV.</title>
<author><name sortKey="Frieman, Matthew" sort="Frieman, Matthew" uniqKey="Frieman M" first="Matthew" last="Frieman">Matthew Frieman</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Basu, Dipanwita" sort="Basu, Dipanwita" uniqKey="Basu D" first="Dipanwita" last="Basu">Dipanwita Basu</name>
</author>
<author><name sortKey="Matthews, Krystal" sort="Matthews, Krystal" uniqKey="Matthews K" first="Krystal" last="Matthews">Krystal Matthews</name>
</author>
<author><name sortKey="Taylor, Justin" sort="Taylor, Justin" uniqKey="Taylor J" first="Justin" last="Taylor">Justin Taylor</name>
</author>
<author><name sortKey="Jones, Grant" sort="Jones, Grant" uniqKey="Jones G" first="Grant" last="Jones">Grant Jones</name>
</author>
<author><name sortKey="Pickles, Raymond" sort="Pickles, Raymond" uniqKey="Pickles R" first="Raymond" last="Pickles">Raymond Pickles</name>
</author>
<author><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name>
</author>
<author><name sortKey="Engel, Daniel A" sort="Engel, Daniel A" uniqKey="Engel D" first="Daniel A" last="Engel">Daniel A. Engel</name>
</author>
</analytic>
<series><title level="j">PloS one</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2011" type="published">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Chlorocebus aethiops</term>
<term>Cloning, Molecular</term>
<term>Culture Media (metabolism)</term>
<term>Drug Design</term>
<term>Epithelial Cells (cytology)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Orthomyxoviridae (genetics)</term>
<term>Phenotype</term>
<term>Protein Binding</term>
<term>SARS Virus (metabolism)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae (virology)</term>
<term>Technology, Pharmaceutical (methods)</term>
<term>Trachea (metabolism)</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antiviraux (pharmacologie)</term>
<term>Cellules HEK293</term>
<term>Cellules Vero</term>
<term>Cellules épithéliales (cytologie)</term>
<term>Clonage moléculaire</term>
<term>Conception de médicament</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Milieux de culture (métabolisme)</term>
<term>Orthomyxoviridae (génétique)</term>
<term>Phénotype</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Saccharomyces cerevisiae (virologie)</term>
<term>Techniques in vitro</term>
<term>Technologie pharmaceutique ()</term>
<term>Trachée (métabolisme)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Culture Media</term>
<term>Green Fluorescent Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules épithéliales</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Epithelial Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
<term>Saccharomyces cerevisiae</term>
<term>Trachea</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Technology, Pharmaceutical</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Milieux de culture</term>
<term>Protéines à fluorescence verte</term>
<term>Saccharomyces cerevisiae</term>
<term>Trachée</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Cloning, Molecular</term>
<term>Drug Design</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>In Vitro Techniques</term>
<term>Phenotype</term>
<term>Protein Binding</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules HEK293</term>
<term>Cellules Vero</term>
<term>Clonage moléculaire</term>
<term>Conception de médicament</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Phénotype</term>
<term>Techniques in vitro</term>
<term>Technologie pharmaceutique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory coronavirus (SARS-CoV) emerged in 2002, resulting in roughly 8000 cases worldwide and 10% mortality. The animal reservoirs for SARS-CoV precursors still exist and the likelihood of future outbreaks in the human population is high. The SARS-CoV papain-like protease (PLP) is an attractive target for pharmaceutical development because it is essential for virus replication and is conserved among human coronaviruses. A yeast-based assay was established for PLP activity that relies on the ability of PLP to induce a pronounced slow-growth phenotype when expressed in S. cerevisiae. Induction of the slow-growth phenotype was shown to take place over a 60-hour time course, providing the basis for conducting a screen for small molecules that restore growth by inhibiting the function of PLP. Five chemical suppressors of the slow-growth phenotype were identified from the 2000 member NIH Diversity Set library. One of these, NSC158362, potently inhibited SARS-CoV replication in cell culture without toxic effects on cells, and it specifically inhibited SARS-CoV replication but not influenza virus replication. The effect of NSC158362 on PLP protease, deubiquitinase and anti-interferon activities was investigated but the compound did not alter these activities. Another suppressor, NSC158011, demonstrated the ability to inhibit PLP protease activity in a cell-based assay. The identification of these inhibitors demonstrated a strong functional connection between the PLP-based yeast assay, the inhibitory compounds, and SARS-CoV biology. Furthermore the data with NSC158362 suggest a novel mechanism for inhibition of SARS-CoV replication that may involve an unknown activity of PLP, or alternatively a direct effect on a cellular target that modifies or bypasses PLP function in yeast and mammalian cells.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22164298</PMID>
<DateCompleted><Year>2012</Year>
<Month>07</Month>
<Day>20</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>6</Volume>
<Issue>12</Issue>
<PubDate><Year>2011</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS ONE</ISOAbbreviation>
</Journal>
<ArticleTitle>Yeast based small molecule screen for inhibitors of SARS-CoV.</ArticleTitle>
<Pagination><MedlinePgn>e28479</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0028479</ELocationID>
<Abstract><AbstractText>Severe acute respiratory coronavirus (SARS-CoV) emerged in 2002, resulting in roughly 8000 cases worldwide and 10% mortality. The animal reservoirs for SARS-CoV precursors still exist and the likelihood of future outbreaks in the human population is high. The SARS-CoV papain-like protease (PLP) is an attractive target for pharmaceutical development because it is essential for virus replication and is conserved among human coronaviruses. A yeast-based assay was established for PLP activity that relies on the ability of PLP to induce a pronounced slow-growth phenotype when expressed in S. cerevisiae. Induction of the slow-growth phenotype was shown to take place over a 60-hour time course, providing the basis for conducting a screen for small molecules that restore growth by inhibiting the function of PLP. Five chemical suppressors of the slow-growth phenotype were identified from the 2000 member NIH Diversity Set library. One of these, NSC158362, potently inhibited SARS-CoV replication in cell culture without toxic effects on cells, and it specifically inhibited SARS-CoV replication but not influenza virus replication. The effect of NSC158362 on PLP protease, deubiquitinase and anti-interferon activities was investigated but the compound did not alter these activities. Another suppressor, NSC158011, demonstrated the ability to inhibit PLP protease activity in a cell-based assay. The identification of these inhibitors demonstrated a strong functional connection between the PLP-based yeast assay, the inhibitory compounds, and SARS-CoV biology. Furthermore the data with NSC158362 suggest a novel mechanism for inhibition of SARS-CoV replication that may involve an unknown activity of PLP, or alternatively a direct effect on a cellular target that modifies or bypasses PLP function in yeast and mammalian cells.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Frieman</LastName>
<ForeName>Matthew</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Basu</LastName>
<ForeName>Dipanwita</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Matthews</LastName>
<ForeName>Krystal</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Taylor</LastName>
<ForeName>Justin</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jones</LastName>
<ForeName>Grant</ForeName>
<Initials>G</Initials>
</Author>
<Author ValidYN="Y"><LastName>Pickles</LastName>
<ForeName>Raymond</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Baric</LastName>
<ForeName>Ralph</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Engel</LastName>
<ForeName>Daniel A</ForeName>
<Initials>DA</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>K22 AI077797</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R21 NS063854</GrantID>
<Acronym>NS</Acronym>
<Agency>NINDS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>1R21NS063854</GrantID>
<Acronym>NS</Acronym>
<Agency>NINDS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>K22AI077797</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2011</Year>
<Month>12</Month>
<Day>02</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D003470">Culture Media</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>147336-22-9</RegistryNumber>
<NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015195" MajorTopicYN="N">Drug Design</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D066298" MajorTopicYN="N">In Vitro Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009975" MajorTopicYN="N">Orthomyxoviridae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013678" MajorTopicYN="N">Technology, Pharmaceutical</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014132" MajorTopicYN="N">Trachea</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year>
<Month>07</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2011</Year>
<Month>11</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2011</Year>
<Month>12</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2011</Year>
<Month>12</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2012</Year>
<Month>7</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">22164298</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0028479</ArticleId>
<ArticleId IdType="pii">PONE-D-11-12499</ArticleId>
<ArticleId IdType="pmc">PMC3229576</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2005 Dec;79(24):15511-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16306622</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Dec;79(24):15189-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16306590</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 2006 Jan;172(1):709-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16157664</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Immunol. 2007;25:443-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17243893</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 Feb;83(4):1881-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19052087</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2009 Jul;83(13):6689-705</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19369340</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2011 Jan;92(Pt 1):60-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20881091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Pathol Lab Med. 2004 Feb;128(2):195-204</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14736283</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Respirology. 2003 Nov;8 Suppl:S9-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15018127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Histopathology. 2004 Aug;45(2):119-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15279629</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Microbiol. 2004 Aug;7(4):412-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15358261</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Physiol (1985). 1990 Sep;69(3):822-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2246169</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomed Pharmacother. 2000 May;54(4):178-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10872716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2002 Jun;76(11):5654-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11991994</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 May 24;361(9371):1761-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12781533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 May 24;361(9371):1773-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12781536</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 Oct 10;302(5643):276-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12958366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2003 Nov;9(11):1453-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14718090</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Dec;78(24):13600-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15564471</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Formos Med Assoc. 2005 Oct;104(10):715-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16385373</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001433 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 001433 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Curation |type= RBID |clé= pubmed:22164298 |texte= Yeast based small molecule screen for inhibitors of SARS-CoV. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:22164298" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |