Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
Identifieur interne : 001296 ( PubMed/Curation ); précédent : 001295; suivant : 001297Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
Auteurs : V M Corman [Allemagne] ; I. Eckerle ; T. Bleicker ; A. Zaki ; O. Landt ; M. Eschbach-Bludau ; S. Van Boheemen ; R. Gopal ; M. Ballhause ; T M Bestebroer ; D. Muth ; M A Müller ; J F Drexler ; M. Zambon ; A D Osterhaus ; R M Fouchier ; C. DrostenSource :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2012.
Descripteurs français
- KwdFr :
- Arabie saoudite, Cadres ouverts de lecture, Coronavirus humain 229E (génétique), Coronavirus humain 229E (isolement et purification), Coronavirus humain NL63 (génétique), Coronavirus humain NL63 (isolement et purification), Coronavirus humain OC43 (génétique), Coronavirus humain OC43 (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (génétique), Infections à coronavirus (virologie), Protéines de l'enveloppe virale, Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Voyage.
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus humain 229E, Coronavirus humain NL63, Coronavirus humain OC43, Infections à coronavirus, Virus du SRAS.
- isolement et purification : Coronavirus humain 229E, Coronavirus humain NL63, Coronavirus humain OC43, Virus du SRAS.
- virologie : Infections à coronavirus.
- Arabie saoudite, Cadres ouverts de lecture, Humains, Protéines de l'enveloppe virale, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Voyage.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Coronavirus 229E, Human (genetics), Coronavirus 229E, Human (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (genetics), Coronavirus Infections (virology), Coronavirus NL63, Human (genetics), Coronavirus NL63, Human (isolation & purification), Coronavirus OC43, Human (genetics), Coronavirus OC43, Human (isolation & purification), Humans, Open Reading Frames, Real-Time Polymerase Chain Reaction (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Saudi Arabia, Sensitivity and Specificity, Travel, Viral Envelope Proteins.
- MESH :
- chemical : Viral Envelope Proteins.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus 229E, Human, Coronavirus Infections, Coronavirus NL63, Human, Coronavirus OC43, Human, SARS Virus.
- isolation & purification : Coronavirus 229E, Human, Coronavirus NL63, Human, Coronavirus OC43, Human, SARS Virus.
- methods : Real-Time Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Humans, Open Reading Frames, Sensitivity and Specificity, Travel.
Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
DOI: 10.2807/ese.17.39.20285-en
PubMed: 23041020
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Drosten</name></author></analytic><series><title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title><idno type="eISSN">1560-7917</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus 229E, Human (genetics)</term><term>Coronavirus 229E, Human (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (genetics)</term><term>Coronavirus Infections (virology)</term><term>Coronavirus NL63, Human (genetics)</term><term>Coronavirus NL63, Human (isolation & purification)</term><term>Coronavirus OC43, Human (genetics)</term><term>Coronavirus OC43, Human (isolation & purification)</term><term>Humans</term><term>Open Reading Frames</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Saudi Arabia</term><term>Sensitivity and Specificity</term><term>Travel</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabie saoudite</term><term>Cadres ouverts de lecture</term><term>Coronavirus humain 229E (génétique)</term><term>Coronavirus humain 229E (isolement et purification)</term><term>Coronavirus humain NL63 (génétique)</term><term>Coronavirus humain NL63 (isolement et purification)</term><term>Coronavirus humain OC43 (génétique)</term><term>Coronavirus humain OC43 (isolement et purification)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (génétique)</term><term>Infections à coronavirus (virologie)</term><term>Protéines de l'enveloppe virale</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sensibilité et spécificité</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Voyage</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus 229E, Human</term><term>Coronavirus Infections</term><term>Coronavirus NL63, Human</term><term>Coronavirus OC43, Human</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus humain 229E</term><term>Coronavirus humain NL63</term><term>Coronavirus humain OC43</term><term>Infections à coronavirus</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus 229E, Human</term><term>Coronavirus NL63, Human</term><term>Coronavirus OC43, Human</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus humain 229E</term><term>Coronavirus humain NL63</term><term>Coronavirus humain OC43</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Open Reading Frames</term><term>Sensitivity and Specificity</term><term>Travel</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arabie saoudite</term><term>Cadres ouverts de lecture</term><term>Humains</term><term>Protéines de l'enveloppe virale</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Voyage</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23041020</PMID><DateCompleted><Year>2013</Year><Month>02</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>08</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1560-7917</ISSN><JournalIssue CitedMedium="Internet"><Volume>17</Volume><Issue>39</Issue><PubDate><Year>2012</Year><Month>Sep</Month><Day>27</Day></PubDate></JournalIssue><Title>Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</Title><ISOAbbreviation>Euro Surveill.</ISOAbbreviation></Journal><ArticleTitle>Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">20285</ELocationID><Abstract><AbstractText>We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Corman</LastName><ForeName>V M</ForeName><Initials>VM</Initials><AffiliationInfo><Affiliation>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eckerle</LastName><ForeName>I</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Bleicker</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Zaki</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Landt</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Eschbach-Bludau</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>van Boheemen</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Gopal</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ballhause</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bestebroer</LastName><ForeName>T M</ForeName><Initials>TM</Initials></Author><Author ValidYN="Y"><LastName>Muth</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>M A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Drexler</LastName><ForeName>J F</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Zambon</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Osterhaus</LastName><ForeName>A D</ForeName><Initials>AD</Initials></Author><Author ValidYN="Y"><LastName>Fouchier</LastName><ForeName>R M</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Drosten</LastName><ForeName>C</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Sweden</Country><MedlineTA>Euro Surveill</MedlineTA><NlmUniqueID>100887452</NlmUniqueID><ISSNLinking>1025-496X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C501689">E protein, SARS coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Euro Surveill. 2012;17(40): pii/20288</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D028941" MajorTopicYN="N">Coronavirus 229E, Human</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058957" MajorTopicYN="N">Coronavirus NL63, Human</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028962" MajorTopicYN="N">Coronavirus OC43, Human</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012529" MajorTopicYN="N" Type="Geographic">Saudi Arabia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014195" MajorTopicYN="N">Travel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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