SrasV1, PubMed, Curation, bibRecord, 000A89

MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.

Identifieur interne : 000A89 ( PubMed/Curation ); précédent : 000A88; suivant : 000A90

MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.

Auteurs : Vineet D. Menachery [États-Unis] ; Hugh D. Mitchell [États-Unis] ; Adam S. Cockrell [États-Unis] ; Lisa E. Gralinski [États-Unis] ; Boyd L. Yount [États-Unis] ; Rachel L. Graham [États-Unis] ; Eileen T. Mcanarney [États-Unis] ; Madeline G. Douglas [États-Unis] ; Trevor Scobey [États-Unis] ; Anne Beall [États-Unis] ; Kenneth Dinnon [États-Unis] ; Jacob F. Kocher [États-Unis] ; Andrew E. Hale [États-Unis] ; Kelly G. Stratton [États-Unis] ; Katrina M. Waters [États-Unis] ; Ralph S. Baric [États-Unis]

Source :

mBio [ 2150-7511 ] ; 2017.

RBID : pubmed:28830941

Descripteurs français

KwdFr :
- Animaux, Cadres ouverts de lecture, Cellules cultivées, Cellules épithéliales (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Facteur de transcription NF-kappa B (métabolisme), Génétique inverse, Humains, Infections à coronavirus (virologie), Inflammation, Interactions hôte-pathogène, Interférons (génétique), Interférons (métabolisme), Lignée cellulaire, Mutation, Réplication virale (génétique), Souris, Transduction du signal.
MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Interférons, Réplication virale.
- métabolisme : Facteur de transcription NF-kappa B, Interférons.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Cellules épithéliales, Infections à coronavirus.
- Animaux, Cadres ouverts de lecture, Cellules cultivées, Génétique inverse, Humains, Inflammation, Interactions hôte-pathogène, Lignée cellulaire, Mutation, Souris, Transduction du signal.

English descriptors

KwdEn :
- Animals, Cell Line, Cells, Cultured, Coronavirus Infections (virology), Epithelial Cells (virology), Host-Pathogen Interactions, Humans, Inflammation, Interferons (genetics), Interferons (metabolism), Mice, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (pathogenicity), Mutation, NF-kappa B (metabolism), Open Reading Frames, Reverse Genetics, Signal Transduction, Virus Replication (genetics).
MESH :
- chemical , genetics : Interferons.
- chemical , metabolism : Interferons, NF-kappa B.
- genetics : Middle East Respiratory Syndrome Coronavirus, Virus Replication.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- virology : Coronavirus Infections, Epithelial Cells.
- Animals, Cell Line, Cells, Cultured, Host-Pathogen Interactions, Humans, Inflammation, Mice, Mutation, Open Reading Frames, Reverse Genetics, Signal Transduction.

Abstract

While dispensable for viral replication, coronavirus (CoV) accessory open reading frame (ORF) proteins often play critical roles during infection and pathogenesis. Utilizing a previously generated mutant, we demonstrate that the absence of all four Middle East respiratory syndrome CoV (MERS-CoV) accessory ORFs (deletion of ORF3, -4a, -4b, and -5 [dORF3-5]) has major implications for viral replication and pathogenesis. Importantly, attenuation of the dORF3-5 mutant is primarily driven by dysregulated host responses, including disrupted cell processes, augmented interferon (IFN) pathway activation, and robust inflammation. In vitro replication attenuation also extends to in vivo models, allowing use of dORF3-5 as a live attenuated vaccine platform. Finally, examination of ORF5 implicates a partial role in modulation of NF-κB-mediated inflammation. Together, the results demonstrate the importance of MERS-CoV accessory ORFs for pathogenesis and highlight them as potential targets for surveillance and therapeutic treatments moving forward.IMPORTANCE The initial emergence and periodic outbreaks of MERS-CoV highlight a continuing threat posed by zoonotic pathogens to global public health. In these studies, mutant virus generation demonstrates the necessity of accessory ORFs in regard to MERS-CoV infection and pathogenesis. With this in mind, accessory ORF functions can be targeted for both therapeutic and vaccine treatments in response to MERS-CoV and related group 2C coronaviruses. In addition, disruption of accessory ORFs in parallel may offer a rapid response platform to attenuation of future emergent strains based on both SARS- and MERS-CoV accessory ORF mutants.

DOI: 10.1128/mBio.00665-17
PubMed: 28830941

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A89

Links to Exploration step

pubmed:28830941

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.</title>
<author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mitchell, Hugh D" sort="Mitchell, Hugh D" uniqKey="Mitchell H" first="Hugh D" last="Mitchell">Hugh D. Mitchell</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S" last="Cockrell">Adam S. Cockrell</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L" last="Graham">Rachel L. Graham</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mcanarney, Eileen T" sort="Mcanarney, Eileen T" uniqKey="Mcanarney E" first="Eileen T" last="Mcanarney">Eileen T. Mcanarney</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Douglas, Madeline G" sort="Douglas, Madeline G" uniqKey="Douglas M" first="Madeline G" last="Douglas">Madeline G. Douglas</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Beall, Anne" sort="Beall, Anne" uniqKey="Beall A" first="Anne" last="Beall">Anne Beall</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dinnon, Kenneth" sort="Dinnon, Kenneth" uniqKey="Dinnon K" first="Kenneth" last="Dinnon">Kenneth Dinnon</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kocher, Jacob F" sort="Kocher, Jacob F" uniqKey="Kocher J" first="Jacob F" last="Kocher">Jacob F. Kocher</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hale, Andrew E" sort="Hale, Andrew E" uniqKey="Hale A" first="Andrew E" last="Hale">Andrew E. Hale</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Stratton, Kelly G" sort="Stratton, Kelly G" uniqKey="Stratton K" first="Kelly G" last="Stratton">Kelly G. Stratton</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Waters, Katrina M" sort="Waters, Katrina M" uniqKey="Waters K" first="Katrina M" last="Waters">Katrina M. Waters</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Rbaric@email.unc.edu.</nlm:affiliation>
<country wicri:rule="url">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:28830941</idno>
<idno type="pmid">28830941</idno>
<idno type="doi">10.1128/mBio.00665-17</idno>
<idno type="wicri:Area/PubMed/Corpus">000A89</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A89</idno>
<idno type="wicri:Area/PubMed/Curation">000A89</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A89</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.</title>
<author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mitchell, Hugh D" sort="Mitchell, Hugh D" uniqKey="Mitchell H" first="Hugh D" last="Mitchell">Hugh D. Mitchell</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cockrell, Adam S" sort="Cockrell, Adam S" uniqKey="Cockrell A" first="Adam S" last="Cockrell">Adam S. Cockrell</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yount, Boyd L" sort="Yount, Boyd L" uniqKey="Yount B" first="Boyd L" last="Yount">Boyd L. Yount</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Graham, Rachel L" sort="Graham, Rachel L" uniqKey="Graham R" first="Rachel L" last="Graham">Rachel L. Graham</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mcanarney, Eileen T" sort="Mcanarney, Eileen T" uniqKey="Mcanarney E" first="Eileen T" last="Mcanarney">Eileen T. Mcanarney</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Douglas, Madeline G" sort="Douglas, Madeline G" uniqKey="Douglas M" first="Madeline G" last="Douglas">Madeline G. Douglas</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Scobey, Trevor" sort="Scobey, Trevor" uniqKey="Scobey T" first="Trevor" last="Scobey">Trevor Scobey</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Beall, Anne" sort="Beall, Anne" uniqKey="Beall A" first="Anne" last="Beall">Anne Beall</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dinnon, Kenneth" sort="Dinnon, Kenneth" uniqKey="Dinnon K" first="Kenneth" last="Dinnon">Kenneth Dinnon</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kocher, Jacob F" sort="Kocher, Jacob F" uniqKey="Kocher J" first="Jacob F" last="Kocher">Jacob F. Kocher</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hale, Andrew E" sort="Hale, Andrew E" uniqKey="Hale A" first="Andrew E" last="Hale">Andrew E. Hale</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Stratton, Kelly G" sort="Stratton, Kelly G" uniqKey="Stratton K" first="Kelly G" last="Stratton">Kelly G. Stratton</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Waters, Katrina M" sort="Waters, Katrina M" uniqKey="Waters K" first="Katrina M" last="Waters">Katrina M. Waters</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Rbaric@email.unc.edu.</nlm:affiliation>
<country wicri:rule="url">États-Unis</country>
<wicri:regionArea>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">mBio</title>
<idno type="eISSN">2150-7511</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cells, Cultured</term>
<term>Coronavirus Infections (virology)</term>
<term>Epithelial Cells (virology)</term>
<term>Host-Pathogen Interactions</term>
<term>Humans</term>
<term>Inflammation</term>
<term>Interferons (genetics)</term>
<term>Interferons (metabolism)</term>
<term>Mice</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term>
<term>Mutation</term>
<term>NF-kappa B (metabolism)</term>
<term>Open Reading Frames</term>
<term>Reverse Genetics</term>
<term>Signal Transduction</term>
<term>Virus Replication (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Cellules cultivées</term>
<term>Cellules épithéliales (virologie)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term>
<term>Facteur de transcription NF-kappa B (métabolisme)</term>
<term>Génétique inverse</term>
<term>Humains</term>
<term>Infections à coronavirus (virologie)</term>
<term>Inflammation</term>
<term>Interactions hôte-pathogène</term>
<term>Interférons (génétique)</term>
<term>Interférons (métabolisme)</term>
<term>Lignée cellulaire</term>
<term>Mutation</term>
<term>Réplication virale (génétique)</term>
<term>Souris</term>
<term>Transduction du signal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferons</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferons</term>
<term>NF-kappa B</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Interférons</term>
<term>Réplication virale</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteur de transcription NF-kappa B</term>
<term>Interférons</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term>
<term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Epithelial Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cells, Cultured</term>
<term>Host-Pathogen Interactions</term>
<term>Humans</term>
<term>Inflammation</term>
<term>Mice</term>
<term>Mutation</term>
<term>Open Reading Frames</term>
<term>Reverse Genetics</term>
<term>Signal Transduction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Cellules cultivées</term>
<term>Génétique inverse</term>
<term>Humains</term>
<term>Inflammation</term>
<term>Interactions hôte-pathogène</term>
<term>Lignée cellulaire</term>
<term>Mutation</term>
<term>Souris</term>
<term>Transduction du signal</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">While dispensable for viral replication, coronavirus (CoV) accessory open reading frame (ORF) proteins often play critical roles during infection and pathogenesis. Utilizing a previously generated mutant, we demonstrate that the absence of all four Middle East respiratory syndrome CoV (MERS-CoV) accessory ORFs (deletion of ORF3, -4a, -4b, and -5 [dORF3-5]) has major implications for viral replication and pathogenesis. Importantly, attenuation of the dORF3-5 mutant is primarily driven by dysregulated host responses, including disrupted cell processes, augmented interferon (IFN) pathway activation, and robust inflammation. <i>In vitro</i>
 replication attenuation also extends to <i>in vivo</i>
 models, allowing use of dORF3-5 as a live attenuated vaccine platform. Finally, examination of ORF5 implicates a partial role in modulation of NF-κB-mediated inflammation. Together, the results demonstrate the importance of MERS-CoV accessory ORFs for pathogenesis and highlight them as potential targets for surveillance and therapeutic treatments moving forward.<b>IMPORTANCE</b>
 The initial emergence and periodic outbreaks of MERS-CoV highlight a continuing threat posed by zoonotic pathogens to global public health. In these studies, mutant virus generation demonstrates the necessity of accessory ORFs in regard to MERS-CoV infection and pathogenesis. With this in mind, accessory ORF functions can be targeted for both therapeutic and vaccine treatments in response to MERS-CoV and related group 2C coronaviruses. In addition, disruption of accessory ORFs in parallel may offer a rapid response platform to attenuation of future emergent strains based on both SARS- and MERS-CoV accessory ORF mutants.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28830941</PMID>
<DateCompleted><Year>2018</Year>
<Month>06</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>27</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2150-7511</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>8</Volume>
<Issue>4</Issue>
<PubDate><Year>2017</Year>
<Month>08</Month>
<Day>22</Day>
</PubDate>
</JournalIssue>
<Title>mBio</Title>
<ISOAbbreviation>mBio</ISOAbbreviation>
</Journal>
<ArticleTitle>MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">e00665-17</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1128/mBio.00665-17</ELocationID>
<Abstract><AbstractText>While dispensable for viral replication, coronavirus (CoV) accessory open reading frame (ORF) proteins often play critical roles during infection and pathogenesis. Utilizing a previously generated mutant, we demonstrate that the absence of all four Middle East respiratory syndrome CoV (MERS-CoV) accessory ORFs (deletion of ORF3, -4a, -4b, and -5 [dORF3-5]) has major implications for viral replication and pathogenesis. Importantly, attenuation of the dORF3-5 mutant is primarily driven by dysregulated host responses, including disrupted cell processes, augmented interferon (IFN) pathway activation, and robust inflammation. <i>In vitro</i>
 replication attenuation also extends to <i>in vivo</i>
 models, allowing use of dORF3-5 as a live attenuated vaccine platform. Finally, examination of ORF5 implicates a partial role in modulation of NF-κB-mediated inflammation. Together, the results demonstrate the importance of MERS-CoV accessory ORFs for pathogenesis and highlight them as potential targets for surveillance and therapeutic treatments moving forward.<b>IMPORTANCE</b>
 The initial emergence and periodic outbreaks of MERS-CoV highlight a continuing threat posed by zoonotic pathogens to global public health. In these studies, mutant virus generation demonstrates the necessity of accessory ORFs in regard to MERS-CoV infection and pathogenesis. With this in mind, accessory ORF functions can be targeted for both therapeutic and vaccine treatments in response to MERS-CoV and related group 2C coronaviruses. In addition, disruption of accessory ORFs in parallel may offer a rapid response platform to attenuation of future emergent strains based on both SARS- and MERS-CoV accessory ORF mutants.</AbstractText>
<CopyrightInformation>Copyright © 2017 Menachery et al.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Menachery</LastName>
<ForeName>Vineet D</ForeName>
<Initials>VD</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mitchell</LastName>
<ForeName>Hugh D</ForeName>
<Initials>HD</Initials>
<AffiliationInfo><Affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cockrell</LastName>
<ForeName>Adam S</ForeName>
<Initials>AS</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gralinski</LastName>
<ForeName>Lisa E</ForeName>
<Initials>LE</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yount</LastName>
<ForeName>Boyd L</ForeName>
<Initials>BL</Initials>
<Suffix>Jr</Suffix>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Graham</LastName>
<ForeName>Rachel L</ForeName>
<Initials>RL</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McAnarney</LastName>
<ForeName>Eileen T</ForeName>
<Initials>ET</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Douglas</LastName>
<ForeName>Madeline G</ForeName>
<Initials>MG</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Scobey</LastName>
<ForeName>Trevor</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Beall</LastName>
<ForeName>Anne</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dinnon</LastName>
<ForeName>Kenneth</ForeName>
<Initials>K</Initials>
<Suffix>3rd</Suffix>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kocher</LastName>
<ForeName>Jacob F</ForeName>
<Initials>JF</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hale</LastName>
<ForeName>Andrew E</ForeName>
<Initials>AE</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Stratton</LastName>
<ForeName>Kelly G</ForeName>
<Initials>KG</Initials>
<AffiliationInfo><Affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Waters</LastName>
<ForeName>Katrina M</ForeName>
<Initials>KM</Initials>
<AffiliationInfo><Affiliation>Pacific Northwest National Laboratory, Richland, Washington, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Baric</LastName>
<ForeName>Ralph S</ForeName>
<Initials>RS</Initials>
<AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Rbaric@email.unc.edu.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>R00 AG049092</GrantID>
<Acronym>AG</Acronym>
<Agency>NIA NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>HHSN272201000019I</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>K99 AG049092</GrantID>
<Acronym>AG</Acronym>
<Agency>NIA NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>U19 AI106772</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>U19 AI100625</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>08</Month>
<Day>22</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>mBio</MedlineTA>
<NlmUniqueID>101519231</NlmUniqueID>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016328">NF-kappa B</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9008-11-1</RegistryNumber>
<NameOfSubstance UI="D007372">Interferons</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007249" MajorTopicYN="N">Inflammation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007372" MajorTopicYN="N">Interferons</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D065207" MajorTopicYN="N">Middle East Respiratory Syndrome Coronavirus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016328" MajorTopicYN="N">NF-kappa B</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016366" MajorTopicYN="Y">Open Reading Frames</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059386" MajorTopicYN="N">Reverse Genetics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">MERS-CoV</Keyword>
<Keyword MajorTopicYN="Y">SARS-CoV</Keyword>
<Keyword MajorTopicYN="Y">coronavirus</Keyword>
<Keyword MajorTopicYN="Y">live vector vaccines</Keyword>
<Keyword MajorTopicYN="Y">reverse genetics</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>8</Month>
<Day>24</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>8</Month>
<Day>24</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>6</Month>
<Day>15</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28830941</ArticleId>
<ArticleId IdType="pii">mBio.00665-17</ArticleId>
<ArticleId IdType="doi">10.1128/mBio.00665-17</ArticleId>
<ArticleId IdType="pmc">PMC5565963</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2014 Jun;88(12):7070-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24719429</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Mar;82(5):2274-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18094188</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3048-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26976607</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2016 Oct 4;7(5):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27703071</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Microbiol. 2016 Nov 28;2:16226</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27892925</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2004 Mar 12;303(5664):1666-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14752165</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Immunol. 2016 Feb;38:109-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26761402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Virol. 2017 Apr;23 :49-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28412285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2013 Oct;19(10 ):1313-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24013700</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2012 Dec;18(12):1820-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23142821</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Virol. 2017 Aug;162(8):2445-2449</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28421366</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2017 Apr 4;8(2):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28377531</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Exp Med Biol. 2006;581:579-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17037601</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virol J. 2015 Sep 11;12:138</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26362536</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Emerg Infect Dis. 2016 Apr;22(4):716-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26981770</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Virol. 2016 Feb;16:55-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26826951</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2014 May 20;5(3):e01174-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24846384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2013 Sep 10;4(5):e00650-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24023385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16157-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24043791</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Nov;85(21):10955-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21865398</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2013 Apr 30;4(3):e00165-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23631916</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 Jan;88(1):717-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24131722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2014 Apr;95(Pt 4):874-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24443473</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Virol. 2012 Jun;2(3):264-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22572391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Nov;87(22):12489-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24027320</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Nov 28;503(7477):535-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24172901</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Dec;79(23):14909-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16282490</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2016 Oct 26;12 (10 ):e1005982</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27783669</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2016 Mar 29;7(2):e00258</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27025250</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2015 Dec;21(12):1508-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26552008</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2015 Sep 5;386(9997):995-1007</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26049252</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2006 Jan 30;24(5):652-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16214268</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2013 Apr;87(7):3885-902</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23365422</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2016 Jan 1;351(6268):77-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26678878</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2016 Aug 12;90(17 ):7798-810</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27334581</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2013 Aug;94(Pt 8):1749-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23620378</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Dec;85(23):12201-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21937658</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>MBio. 2013 Feb 19;4(1):e00611-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23422412</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2014 May;88(9):4866-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24522921</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2015 Oct 29;11(10):e1005215</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26513244</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Dec 30;90(6):3253-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26719272</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A89 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 000A89 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PubMed
   |étape=   Curation
   |type=    RBID
   |clé=     pubmed:28830941
   |texte=   MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i   -Sk "pubmed:28830941" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.

MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki