A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase.
Identifieur interne : 000A78 ( PubMed/Curation ); précédent : 000A77; suivant : 000A79A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase.
Auteurs : Jin-Moo Lee [Corée du Sud] ; Jin-Beom Cho [Corée du Sud] ; Hee-Chul Ahn [Corée du Sud] ; Woong Jung [Corée du Sud] ; Yong-Joo Jeong [Corée du Sud]Source :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2017.
Descripteurs français
- KwdFr :
- ADN (antagonistes et inhibiteurs), Adénosine triphosphate, Antienzymes (), Antienzymes (isolement et purification), Antienzymes (pharmacologie), Antiviraux (), Antiviraux (antagonistes et inhibiteurs), Antiviraux (isolement et purification), Concentration inhibitrice 50, Helicase (), Humains, Hydrolyse, Lignée cellulaire (), Survie cellulaire (), Virus du SRAS (), Virus du SRAS (enzymologie).
- MESH :
- antagonistes et inhibiteurs : ADN, Antiviraux.
- enzymologie : Virus du SRAS.
- isolement et purification : Antienzymes, Antiviraux.
- pharmacologie : Antienzymes.
- Adénosine triphosphate, Antienzymes, Antiviraux, Concentration inhibitrice 50, Helicase, Humains, Hydrolyse, Lignée cellulaire, Survie cellulaire, Virus du SRAS.
English descriptors
- KwdEn :
- Adenosine Triphosphate, Antiviral Agents (antagonists & inhibitors), Antiviral Agents (chemistry), Antiviral Agents (isolation & purification), Cell Line (drug effects), Cell Survival (drug effects), DNA (antagonists & inhibitors), DNA Helicases (drug effects), Enzyme Inhibitors (chemistry), Enzyme Inhibitors (isolation & purification), Enzyme Inhibitors (pharmacology), Humans, Hydrolysis, Inhibitory Concentration 50, SARS Virus (drug effects), SARS Virus (enzymology).
- MESH :
- chemical , antagonists & inhibitors : Antiviral Agents, DNA.
- chemical , chemistry : Antiviral Agents, Enzyme Inhibitors.
- chemical , drug effects : DNA Helicases.
- chemical , isolation & purification : Antiviral Agents, Enzyme Inhibitors.
- chemical , pharmacology : Enzyme Inhibitors.
- chemical : Adenosine Triphosphate.
- drug effects : Cell Line, Cell Survival, SARS Virus.
- enzymology : SARS Virus.
- Humans, Hydrolysis, Inhibitory Concentration 50.
Abstract
We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC50 values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 µM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.
DOI: 10.4014/jmb.1707.07073
PubMed: 28910865
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University, Seoul 02707</wicri:regionArea></affiliation></author><author><name sortKey="Ahn, Hee Chul" sort="Ahn, Hee Chul" uniqKey="Ahn H" first="Hee-Chul" last="Ahn">Hee-Chul Ahn</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacy, Dongguk University-Seoul, Gyeonggi-do 10326, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Pharmacy, Dongguk University-Seoul, Gyeonggi-do 10326</wicri:regionArea></affiliation></author><author><name sortKey="Jung, Woong" sort="Jung, Woong" uniqKey="Jung W" first="Woong" last="Jung">Woong Jung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278</wicri:regionArea></affiliation></author><author><name sortKey="Jeong, Yong Joo" sort="Jeong, Yong Joo" uniqKey="Jeong Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Chemistry, Kookmin University, Seoul 02707, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Applied Chemistry, Kookmin University, Seoul 02707</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="eISSN">1738-8872</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate</term><term>Antiviral Agents (antagonists & inhibitors)</term><term>Antiviral Agents (chemistry)</term><term>Antiviral Agents (isolation & purification)</term><term>Cell Line (drug effects)</term><term>Cell Survival (drug effects)</term><term>DNA (antagonists & inhibitors)</term><term>DNA Helicases (drug effects)</term><term>Enzyme Inhibitors (chemistry)</term><term>Enzyme Inhibitors (isolation & purification)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Humans</term><term>Hydrolysis</term><term>Inhibitory Concentration 50</term><term>SARS Virus (drug effects)</term><term>SARS Virus (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (antagonistes et inhibiteurs)</term><term>Adénosine triphosphate</term><term>Antienzymes ()</term><term>Antienzymes (isolement et purification)</term><term>Antienzymes (pharmacologie)</term><term>Antiviraux ()</term><term>Antiviraux (antagonistes et inhibiteurs)</term><term>Antiviraux (isolement et purification)</term><term>Concentration inhibitrice 50</term><term>Helicase ()</term><term>Humains</term><term>Hydrolyse</term><term>Lignée cellulaire ()</term><term>Survie cellulaire ()</term><term>Virus du SRAS ()</term><term>Virus du SRAS (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Antiviral Agents</term><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>DNA Helicases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiviral Agents</term><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Adenosine Triphosphate</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>ADN</term><term>Antiviraux</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Line</term><term>Cell Survival</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antienzymes</term><term>Antiviraux</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Hydrolysis</term><term>Inhibitory Concentration 50</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adénosine triphosphate</term><term>Antienzymes</term><term>Antiviraux</term><term>Concentration inhibitrice 50</term><term>Helicase</term><term>Humains</term><term>Hydrolyse</term><term>Lignée cellulaire</term><term>Survie cellulaire</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-<i>N</i>-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC<sub>50</sub> values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 µM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28910865</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>07</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1738-8872</ISSN><JournalIssue CitedMedium="Internet"><Volume>27</Volume><Issue>11</Issue><PubDate><Year>2017</Year><Month>Nov</Month><Day>28</Day></PubDate></JournalIssue><Title>Journal of microbiology and biotechnology</Title><ISOAbbreviation>J. Microbiol. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase.</ArticleTitle><Pagination><MedlinePgn>2070-2073</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.4014/jmb.1707.07073</ELocationID><Abstract><AbstractText>We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-<i>N</i>-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC<sub>50</sub> values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 µM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jin-Moo</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Applied Chemistry, Kookmin University, Seoul 02707, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Jin-Beom</ForeName><Initials>JB</Initials><AffiliationInfo><Affiliation>Department of Applied Chemistry, Kookmin University, Seoul 02707, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Hee-Chul</ForeName><Initials>HC</Initials><AffiliationInfo><Affiliation>Department of Pharmacy, Dongguk University-Seoul, Gyeonggi-do 10326, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jung</LastName><ForeName>Woong</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Emergency Medicine, Kyung Hee University Hospital at Gangdong, Seoul 05278, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jeong</LastName><ForeName>Yong-Joo</ForeName><Initials>YJ</Initials><AffiliationInfo><Affiliation>Department of Applied Chemistry, Kookmin University, Seoul 02707, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>J Microbiol Biotechnol</MedlineTA><NlmUniqueID>9431852</NlmUniqueID><ISSNLinking>1017-7825</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>8L70Q75FXE</RegistryNumber><NameOfSubstance UI="D000255">Adenosine Triphosphate</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.6.4.-</RegistryNumber><NameOfSubstance UI="D004265">DNA Helicases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000255" MajorTopicYN="N">Adenosine Triphosphate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004265" MajorTopicYN="N">DNA Helicases</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020128" MajorTopicYN="N">Inhibitory Concentration 50</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ATP hydrolysis</Keyword><Keyword MajorTopicYN="N">SARS</Keyword><Keyword MajorTopicYN="N">cytotoxicity</Keyword><Keyword MajorTopicYN="N">dsDNA unwinding</Keyword><Keyword MajorTopicYN="N">helicase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28910865</ArticleId><ArticleId IdType="pii">10.4014/jmb.1707.07073</ArticleId><ArticleId IdType="doi">10.4014/jmb.1707.07073</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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