The establishment of reference sequence for SARS-CoV-2 and variation analysis.
Identifieur interne : 000636 ( PubMed/Curation ); précédent : 000635; suivant : 000637The establishment of reference sequence for SARS-CoV-2 and variation analysis.
Auteurs : Changtai Wang [République populaire de Chine] ; Zhongping Liu [République populaire de Chine] ; Zixiang Chen [République populaire de Chine] ; Xin Huang [République populaire de Chine] ; Mengyuan Xu [République populaire de Chine] ; Tengfei He [République populaire de Chine] ; Zhenhua Zhang [République populaire de Chine]Source :
- Journal of medical virology [ 1096-9071 ] ; 2020.
Abstract
Starting around December 2019, an epidemic of pneumonia, which was named COVID-19 by the World Health Organization, broke out in Wuhan, China, and is spreading throughout the world. A new coronavirus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by the Coronavirus Study Group of the International Committee on Taxonomy of Viruses was soon found to be the cause. At present, the sensitivity of clinical nucleic acid detection is limited, and it is still unclear whether it is related to genetic variation. In this study, we retrieved 95 full-length genomic sequences of SARAS-CoV-2 strains from the National Center for Biotechnology Information and GISAID databases, established the reference sequence by conducting multiple sequence alignment and phylogenetic analyses, and analyzed sequence variations along the SARS-CoV-2 genome. The homology among all viral strains was generally high, among them, 99.99% (99.91%-100%) at the nucleotide level and 99.99% (99.79%-100%) at the amino acid level. Although overall variation in open-reading frame (ORF) regions is low, 13 variation sites in 1a, 1b, S, 3a, M, 8, and N regions were identified, among which positions nt28144 in ORF 8 and nt8782 in ORF 1a showed mutation rate of 30.53% (29/95) and 29.47% (28/95), respectively. These findings suggested that there may be selective mutations in SARS-COV-2, and it is necessary to avoid certain regions when designing primers and probes. Establishment of the reference sequence for SARS-CoV-2 could benefit not only biological study of this virus but also diagnosis, clinical monitoring and intervention of SARS-CoV-2 infection in the future.
DOI: 10.1002/jmv.25762
PubMed: 32167180
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000636
Links to Exploration step
pubmed:32167180Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The establishment of reference sequence for SARS-CoV-2 and variation analysis.</title><author><name sortKey="Wang, Changtai" sort="Wang, Changtai" uniqKey="Wang C" first="Changtai" last="Wang">Changtai Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Zhongping" sort="Liu, Zhongping" uniqKey="Liu Z" first="Zhongping" last="Liu">Zhongping Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Zixiang" sort="Chen, Zixiang" uniqKey="Chen Z" first="Zixiang" last="Chen">Zixiang Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Huang, Xin" sort="Huang, Xin" uniqKey="Huang X" first="Xin" last="Huang">Xin Huang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Mengyuan" sort="Xu, Mengyuan" uniqKey="Xu M" first="Mengyuan" last="Xu">Mengyuan Xu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="He, Tengfei" sort="He, Tengfei" uniqKey="He T" first="Tengfei" last="He">Tengfei He</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Zhenhua" sort="Zhang, Zhenhua" uniqKey="Zhang Z" first="Zhenhua" last="Zhang">Zhenhua Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32167180</idno><idno type="pmid">32167180</idno><idno type="doi">10.1002/jmv.25762</idno><idno type="wicri:Area/PubMed/Corpus">000636</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000636</idno><idno type="wicri:Area/PubMed/Curation">000636</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000636</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The establishment of reference sequence for SARS-CoV-2 and variation analysis.</title><author><name sortKey="Wang, Changtai" sort="Wang, Changtai" uniqKey="Wang C" first="Changtai" last="Wang">Changtai Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Zhongping" sort="Liu, Zhongping" uniqKey="Liu Z" first="Zhongping" last="Liu">Zhongping Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Zixiang" sort="Chen, Zixiang" uniqKey="Chen Z" first="Zixiang" last="Chen">Zixiang Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Huang, Xin" sort="Huang, Xin" uniqKey="Huang X" first="Xin" last="Huang">Xin Huang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Mengyuan" sort="Xu, Mengyuan" uniqKey="Xu M" first="Mengyuan" last="Xu">Mengyuan Xu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="He, Tengfei" sort="He, Tengfei" uniqKey="He T" first="Tengfei" last="He">Tengfei He</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Zhenhua" sort="Zhang, Zhenhua" uniqKey="Zhang Z" first="Zhenhua" last="Zhang">Zhenhua Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of medical virology</title><idno type="eISSN">1096-9071</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Starting around December 2019, an epidemic of pneumonia, which was named COVID-19 by the World Health Organization, broke out in Wuhan, China, and is spreading throughout the world. A new coronavirus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by the Coronavirus Study Group of the International Committee on Taxonomy of Viruses was soon found to be the cause. At present, the sensitivity of clinical nucleic acid detection is limited, and it is still unclear whether it is related to genetic variation. In this study, we retrieved 95 full-length genomic sequences of SARAS-CoV-2 strains from the National Center for Biotechnology Information and GISAID databases, established the reference sequence by conducting multiple sequence alignment and phylogenetic analyses, and analyzed sequence variations along the SARS-CoV-2 genome. The homology among all viral strains was generally high, among them, 99.99% (99.91%-100%) at the nucleotide level and 99.99% (99.79%-100%) at the amino acid level. Although overall variation in open-reading frame (ORF) regions is low, 13 variation sites in 1a, 1b, S, 3a, M, 8, and N regions were identified, among which positions nt28144 in ORF 8 and nt8782 in ORF 1a showed mutation rate of 30.53% (29/95) and 29.47% (28/95), respectively. These findings suggested that there may be selective mutations in SARS-COV-2, and it is necessary to avoid certain regions when designing primers and probes. Establishment of the reference sequence for SARS-CoV-2 could benefit not only biological study of this virus but also diagnosis, clinical monitoring and intervention of SARS-CoV-2 infection in the future.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32167180</PMID><DateRevised><Year>2020</Year><Month>03</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-9071</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Mar</Month><Day>13</Day></PubDate></JournalIssue><Title>Journal of medical virology</Title><ISOAbbreviation>J. Med. Virol.</ISOAbbreviation></Journal><ArticleTitle>The establishment of reference sequence for SARS-CoV-2 and variation analysis.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1002/jmv.25762</ELocationID><Abstract><AbstractText>Starting around December 2019, an epidemic of pneumonia, which was named COVID-19 by the World Health Organization, broke out in Wuhan, China, and is spreading throughout the world. A new coronavirus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by the Coronavirus Study Group of the International Committee on Taxonomy of Viruses was soon found to be the cause. At present, the sensitivity of clinical nucleic acid detection is limited, and it is still unclear whether it is related to genetic variation. In this study, we retrieved 95 full-length genomic sequences of SARAS-CoV-2 strains from the National Center for Biotechnology Information and GISAID databases, established the reference sequence by conducting multiple sequence alignment and phylogenetic analyses, and analyzed sequence variations along the SARS-CoV-2 genome. The homology among all viral strains was generally high, among them, 99.99% (99.91%-100%) at the nucleotide level and 99.99% (99.79%-100%) at the amino acid level. Although overall variation in open-reading frame (ORF) regions is low, 13 variation sites in 1a, 1b, S, 3a, M, 8, and N regions were identified, among which positions nt28144 in ORF 8 and nt8782 in ORF 1a showed mutation rate of 30.53% (29/95) and 29.47% (28/95), respectively. These findings suggested that there may be selective mutations in SARS-COV-2, and it is necessary to avoid certain regions when designing primers and probes. Establishment of the reference sequence for SARS-CoV-2 could benefit not only biological study of this virus but also diagnosis, clinical monitoring and intervention of SARS-CoV-2 infection in the future.</AbstractText><CopyrightInformation>© 2020 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Changtai</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Infectious Diseases, The Affiliated Anqing Hospital of Anhui Medical University, Anqing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Zhongping</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zixiang</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Mengyuan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Tengfei</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zhenhua</ForeName><Initials>Z</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8480-9004</Identifier><AffiliationInfo><Affiliation>Department of Infectious Diseases, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Clinical Virology, The Second Hospital of Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Anhui Provincial Laboratory of Inflammatory and Immunity Disease, Anhui Medical University, Hefei, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2020Z1001</GrantID><Agency>Emergency Science and Technology Plan for Novel Coronavirus Infection of Anqing</Agency><Country></Country></Grant><Grant><GrantID>1608085MH162</GrantID><Agency>Anhui Provincial Natural Science Foundation of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Med Virol</MedlineTA><NlmUniqueID>7705876</NlmUniqueID><ISSNLinking>0146-6615</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">homology</Keyword><Keyword MajorTopicYN="N">nucleotide</Keyword><Keyword MajorTopicYN="N">reference sequence</Keyword><Keyword MajorTopicYN="N">variation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32167180</ArticleId><ArticleId IdType="doi">10.1002/jmv.25762</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395:497-506.</Citation></Reference><Reference><Citation>Lu R, Zhao X, Li J, et al. Genomic characterization and epidemiology of 2019 novel coronavirus: implications of virus origins and receptor binding. Lancet. 2020;395:565-574. https://doi.org/10.1016/S0140-6736(20)30251-8</Citation></Reference><Reference><Citation>Zhu N, Zhang D, Wang W, et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382:727-733.</Citation></Reference><Reference><Citation>National Health Commission of the People's Republic of China. http://www.nhc.gov.cn (Assessed on March 8th, 2020).</Citation></Reference><Reference><Citation>WHO main website. https://www.who.int (accessed March 8th, 2020).</Citation></Reference><Reference><Citation>The Novel Coronavirus Pneumonia Emergency Response Epidemiology Team. The epidemiological characteristics of an outbreak of 2019 novel coronavirus diseases (COVID-19) in china. Chin J Epidemiol. 2020;41:145-151.</Citation></Reference><Reference><Citation>Chan JFW, Yuan S, Kok KH, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020;395:514-523.</Citation></Reference><Reference><Citation>Li Q, Guan X, Wu P, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. N Engl J Med. 2020. https://doi.org/10.1056/NEJMoa2001316</Citation></Reference><Reference><Citation>Chen N, Zhou M, Dong X, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet. 2020;395:507-513.</Citation></Reference><Reference><Citation>Guan WJ, Ni Z, Hu Y, et al. Clinical characteristics of 2019 novel coronavirus infection in China. medRxiv. 2020:20020974. https://doi.org/10.1101/2020.02.06.20020974</Citation></Reference><Reference><Citation>Wu F, Zhao S, Yu B, et al. A new coronavirus associated with human respiratory disease in China. Nature. 2020;579:265-269.</Citation></Reference><Reference><Citation>Su S, Wong G, Shi W, et al. Epidemiology, genetic recombination, and pathogenesis of coronaviruses. Trends Microbiol. 2016;24:490-502.</Citation></Reference><Reference><Citation>Cheng VC, Lau SK, Woo PC, Yuen KY. Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection. Clin Microbiol Rev. 2007;20:660-694.</Citation></Reference><Reference><Citation>Chan JFW, Lau SKP, To KKW, Cheng VCC, Woo PCY, Yuen K. Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease. Clin Microbiol Rev. 2015;28:465-522.</Citation></Reference><Reference><Citation>Zhang N, Wang L, Deng X. Recent advances in the detection of respiratory virus infection in humans. J Med Virol. 2020;92(4):408-417.</Citation></Reference><Reference><Citation>Chu D, Pan Y, Cheng S, et al. Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. Clin Chem. 2020:hvaa029.</Citation></Reference><Reference><Citation>Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25:2000045.</Citation></Reference><Reference><Citation>Zhou P, Yang X, Wang X, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579:270-273.</Citation></Reference><Reference><Citation>Xiao KP, Zhai JP, Feng YY, et al. Isolation and characterization of 2019-nCoV-like coronavirus from Malayan pangolins. bioRxiv. 2020:951335. https://doi.org/10.1101/2020.02.17.951335</Citation></Reference><Reference><Citation>Lam T, Shum M, Zhu HZ, et al. Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China. bioRxiv. 2020:945485. https://doi.org/10.1101/2020.02.13.945485</Citation></Reference><Reference><Citation>Chen L, Shi Y, Yang W, et al. Differences in Cpg island distribution between subgenotypes of the hepatitis B virus genotype. Med Sci Monitor. 2018;24:6781-6794.</Citation></Reference><Reference><Citation>Zhang Z, Xia J, Sun B, et al. In vitro and in vivo replication of a chemically synthesized consensus genome of hepatitis B virus genotype B. J Virol Methods. 2015;213:57-64.</Citation></Reference><Reference><Citation>Chan JFW, Kok KH, Zhu Z, et al. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan. Emerg Microbes Infec. 2020;9:221-236.</Citation></Reference><Reference><Citation>Wang S, Bo K, Ma JL, et al. A deep learning algorithm using CT images to screen for Corona Virus Disease (COVID-19). medRxiv. 2020:20023028. https://doi.org/10.1101/2020.02.14.20023028</Citation></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000636 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 000636 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:32167180
|texte= The establishment of reference sequence for SARS-CoV-2 and variation analysis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:32167180" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |