An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.
Identifieur interne : 000153 ( PubMed/Curation ); précédent : 000152; suivant : 000154An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.
Auteurs : Anna S. Fomsgaard [Danemark] ; Maiken Wors E Rosenstierne [Danemark]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), ARN viral (isolement et purification), Coronavirus (génétique), Coronavirus (isolement et purification), Danemark, Flux de travaux, Humains, Infections à coronavirus (diagnostic), Pandémies, Partie orale du pharynx (virologie), Pneumopathie virale (diagnostic), RT-PCR (), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic).
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus, Pneumopathie virale, Syndrome respiratoire aigu sévère.
- génétique : ARN viral, Coronavirus.
- isolement et purification : ARN viral, Coronavirus.
- virologie : Partie orale du pharynx.
- Danemark, Flux de travaux, Humains, Pandémies, RT-PCR, Sensibilité et spécificité.
- Wicri :
- geographic : Danemark.
English descriptors
- KwdEn :
- Betacoronavirus, Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Denmark, Humans, Oropharynx (virology), Pandemics, Pneumonia, Viral (diagnosis), RNA, Viral (analysis), RNA, Viral (genetics), RNA, Viral (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Workflow.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical , isolation & purification : RNA, Viral.
- geographic : Denmark.
- diagnosis : Coronavirus Infections, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Oropharynx.
- Betacoronavirus, Humans, Pandemics, Sensitivity and Specificity, Workflow.
Abstract
The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.
DOI: 10.2807/1560-7917.ES.2020.25.14.2000398
PubMed: 32290902
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pubmed:32290902Le document en format XML
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Fomsgaard</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen</wicri:regionArea></affiliation></author><author><name sortKey="Rosenstierne, Maiken Wors E" sort="Rosenstierne, Maiken Wors E" uniqKey="Rosenstierne M" first="Maiken Wors E" last="Rosenstierne">Maiken Wors E Rosenstierne</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32290902</idno><idno type="pmid">32290902</idno><idno type="doi">10.2807/1560-7917.ES.2020.25.14.2000398</idno><idno type="wicri:Area/PubMed/Corpus">000153</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000153</idno><idno type="wicri:Area/PubMed/Curation">000153</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000153</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.</title><author><name sortKey="Fomsgaard, Anna S" sort="Fomsgaard, Anna S" uniqKey="Fomsgaard A" first="Anna S" last="Fomsgaard">Anna S. 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Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32290902</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>20</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1560-7917</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>14</Issue><PubDate><Year>2020</Year><Month>04</Month></PubDate></JournalIssue><Title>Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</Title><ISOAbbreviation>Euro Surveill.</ISOAbbreviation></Journal><ArticleTitle>An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.2807/1560-7917.ES.2020.25.14.2000398</ELocationID><Abstract><AbstractText>The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fomsgaard</LastName><ForeName>Anna S</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosenstierne</LastName><ForeName>Maiken Worsøe</ForeName><Initials>MW</Initials><AffiliationInfo><Affiliation>Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Sweden</Country><MedlineTA>Euro Surveill</MedlineTA><NlmUniqueID>100887452</NlmUniqueID><ISSNLinking>1025-496X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName><SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003718" MajorTopicYN="N" Type="Geographic">Denmark</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009960" MajorTopicYN="N">Oropharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057188" MajorTopicYN="N">Workflow</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COVID-19</Keyword><Keyword MajorTopicYN="Y">RT-qPCR</Keyword><Keyword MajorTopicYN="Y">SARS-CoV-2</Keyword><Keyword MajorTopicYN="Y">molecular diagnostics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32290902</ArticleId><ArticleId IdType="doi">10.2807/1560-7917.ES.2020.25.14.2000398</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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