Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Identifieur interne : 000122 ( PubMed/Curation ); précédent : 000121; suivant : 000123Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Auteurs : Giwan Seo [Corée du Sud] ; Geonhee Lee [Corée du Sud] ; Mi Jeong Kim [Corée du Sud] ; Seung-Hwa Baek [Corée du Sud] ; Minsuk Choi [Corée du Sud] ; Keun Bon Ku [Corée du Sud] ; Chang-Seop Lee [Corée du Sud] ; Sangmi Jun [Corée du Sud] ; Daeui Park [Corée du Sud] ; Hong Gi Kim [Corée du Sud] ; Seong-Jun Kim [Corée du Sud] ; Jeong-O Lee [Corée du Sud] ; Bum Tae Kim [Corée du Sud] ; Edmond Changkyun Park [Corée du Sud] ; Seung Il Kim [Corée du Sud]Source :
- ACS nano [ 1936-086X ] ; 2020.
Abstract
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.
DOI: 10.1021/acsnano.0c02823
PubMed: 32293168
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Gi" sort="Kim, Hong Gi" uniqKey="Kim H" first="Hong Gi" last="Kim">Hong Gi Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Lee, Jeong O" sort="Lee, Jeong O" uniqKey="Lee J" first="Jeong-O" last="Lee">Jeong-O Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum Tae" last="Kim">Bum Tae Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus 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Seo</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea></affiliation></author><author><name sortKey="Lee, Geonhee" sort="Lee, Geonhee" uniqKey="Lee G" first="Geonhee" last="Lee">Geonhee Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Mi Jeong" sort="Kim, Mi Jeong" uniqKey="Kim M" first="Mi Jeong" last="Kim">Mi Jeong Kim</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea></affiliation></author><author><name sortKey="Baek, Seung Hwa" sort="Baek, Seung Hwa" uniqKey="Baek S" first="Seung-Hwa" last="Baek">Seung-Hwa Baek</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Choi, Minsuk" sort="Choi, Minsuk" uniqKey="Choi M" first="Minsuk" last="Choi">Minsuk Choi</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Ku, Keun Bon" sort="Ku, Keun Bon" uniqKey="Ku K" first="Keun Bon" last="Ku">Keun Bon Ku</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Lee, Chang Seop" sort="Lee, Chang Seop" uniqKey="Lee C" first="Chang-Seop" last="Lee">Chang-Seop Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986</wicri:regionArea></affiliation></author><author><name sortKey="Jun, Sangmi" sort="Jun, Sangmi" uniqKey="Jun S" first="Sangmi" last="Jun">Sangmi Jun</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Park, Daeui" sort="Park, Daeui" uniqKey="Park D" first="Daeui" last="Park">Daeui Park</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Hong Gi" sort="Kim, Hong Gi" uniqKey="Kim H" first="Hong Gi" last="Kim">Hong Gi Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Lee, Jeong O" sort="Lee, Jeong O" uniqKey="Lee J" first="Jeong-O" last="Lee">Jeong-O Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum Tae" last="Kim">Bum Tae Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea></affiliation></author><author><name sortKey="Park, Edmond Changkyun" sort="Park, Edmond Changkyun" uniqKey="Park E" first="Edmond Changkyun" last="Park">Edmond Changkyun Park</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea></affiliation></author></analytic><series><title level="j">ACS nano</title><idno type="eISSN">1936-086X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 10<sup>1</sup> pfu/mL) and clinical samples (LOD: 2.42 × 10<sup>2</sup> copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32293168</PMID><DateRevised><Year>2020</Year><Month>04</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1936-086X</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Apr</Month><Day>20</Day></PubDate></JournalIssue><Title>ACS nano</Title><ISOAbbreviation>ACS Nano</ISOAbbreviation></Journal><ArticleTitle>Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1021/acsnano.0c02823</ELocationID><Abstract><AbstractText>Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 10<sup>1</sup> pfu/mL) and clinical samples (LOD: 2.42 × 10<sup>2</sup> copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Seo</LastName><ForeName>Giwan</ForeName><Initials>G</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8734-6199</Identifier><AffiliationInfo><Affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Geonhee</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Mi Jeong</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baek</LastName><ForeName>Seung-Hwa</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Minsuk</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ku</LastName><ForeName>Keun Bon</ForeName><Initials>KB</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Chang-Seop</ForeName><Initials>CS</Initials><AffiliationInfo><Affiliation>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju 54907, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jun</LastName><ForeName>Sangmi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Research Equipment, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Daeui</ForeName><Initials>D</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-9452-5849</Identifier><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hong Gi</ForeName><Initials>HG</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Seong-Jun</ForeName><Initials>SJ</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jeong-O</ForeName><Initials>JO</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7343-4892</Identifier><AffiliationInfo><Affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Bum Tae</ForeName><Initials>BT</Initials><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Edmond Changkyun</ForeName><Initials>EC</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6699-4886</Identifier><AffiliationInfo><Affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Bio-Analysis Science, University of Science & Technology (UST), Daejeon 34113, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Seung Il</ForeName><Initials>SI</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6681-4486</Identifier><AffiliationInfo><Affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Bio-Analysis Science, University of Science & Technology (UST), Daejeon 34113, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Nano</MedlineTA><NlmUniqueID>101313589</NlmUniqueID><ISSNLinking>1936-0851</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">2019-nCoV</Keyword><Keyword MajorTopicYN="N">COVID-19</Keyword><Keyword MajorTopicYN="N">FET</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">biosensor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32293168</ArticleId><ArticleId IdType="doi">10.1021/acsnano.0c02823</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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