A highly conserved epitope on the spike protein of infectious bronchitis virus.
Identifieur interne : 003523 ( PubMed/Corpus ); précédent : 003522; suivant : 003524A highly conserved epitope on the spike protein of infectious bronchitis virus.
Auteurs : L. Wang ; R L Parr ; D J King ; E W CollissonSource :
- Archives of virology [ 0304-8608 ] ; 1995.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Chickens, Conserved Sequence, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Epitopes (analysis), Europe, Hemagglutinins, Viral (genetics), Hemagglutinins, Viral (immunology), Immunoblotting, Infectious bronchitis virus (genetics), Infectious bronchitis virus (immunology), Japan, Membrane Glycoproteins (analysis), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Molecular Sequence Data, Protein Structure, Secondary, Sequence Homology, Amino Acid, Spike Glycoprotein, Coronavirus, United States, Viral Envelope Proteins (analysis), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , analysis : Epitopes, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Hemagglutinins, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Hemagglutinins, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical : Antibodies, Monoclonal, Spike Glycoprotein, Coronavirus.
- geographic : Europe, Japan, United States.
- genetics : Infectious bronchitis virus.
- immunology : Infectious bronchitis virus.
- Amino Acid Sequence, Animals, Chickens, Conserved Sequence, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Immunoblotting, Molecular Sequence Data, Protein Structure, Secondary, Sequence Homology, Amino Acid.
Abstract
The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV.
DOI: 10.1007/bf01323240
PubMed: 8572941
Links to Exploration step
pubmed:8572941Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A highly conserved epitope on the spike protein of infectious bronchitis virus.</title><author><name sortKey="Wang, L" sort="Wang, L" uniqKey="Wang L" first="L" last="Wang">L. Wang</name><affiliation><nlm:affiliation>Department of Veterinary Pathobiology, Texas A&M University, College Station, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Parr, R L" sort="Parr, R L" uniqKey="Parr R" first="R L" last="Parr">R L Parr</name></author><author><name sortKey="King, D J" sort="King, D J" uniqKey="King D" first="D J" last="King">D J King</name></author><author><name sortKey="Collisson, E W" sort="Collisson, E W" uniqKey="Collisson E" first="E W" last="Collisson">E W Collisson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:8572941</idno><idno type="pmid">8572941</idno><idno type="doi">10.1007/bf01323240</idno><idno type="wicri:Area/PubMed/Corpus">003523</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003523</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A highly conserved epitope on the spike protein of infectious bronchitis virus.</title><author><name sortKey="Wang, L" sort="Wang, L" uniqKey="Wang L" first="L" last="Wang">L. Wang</name><affiliation><nlm:affiliation>Department of Veterinary Pathobiology, Texas A&M University, College Station, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Parr, R L" sort="Parr, R L" uniqKey="Parr R" first="R L" last="Parr">R L Parr</name></author><author><name sortKey="King, D J" sort="King, D J" uniqKey="King D" first="D J" last="King">D J King</name></author><author><name sortKey="Collisson, E W" sort="Collisson, E W" uniqKey="Collisson E" first="E W" last="Collisson">E W Collisson</name></author></analytic><series><title level="j">Archives of virology</title><idno type="ISSN">0304-8608</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal</term><term>Chickens</term><term>Conserved Sequence</term><term>Cross Reactions</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes (analysis)</term><term>Europe</term><term>Hemagglutinins, Viral (genetics)</term><term>Hemagglutinins, Viral (immunology)</term><term>Immunoblotting</term><term>Infectious bronchitis virus (genetics)</term><term>Infectious bronchitis virus (immunology)</term><term>Japan</term><term>Membrane Glycoproteins (analysis)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term><term>Sequence Homology, Amino Acid</term><term>Spike Glycoprotein, Coronavirus</term><term>United States</term><term>Viral Envelope Proteins (analysis)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Epitopes</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinins, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Hemagglutinins, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Europe</term><term>Japan</term><term>United States</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chickens</term><term>Conserved Sequence</term><term>Cross Reactions</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunoblotting</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8572941</PMID><DateCompleted><Year>1996</Year><Month>03</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0304-8608</ISSN><JournalIssue CitedMedium="Print"><Volume>140</Volume><Issue>12</Issue><PubDate><Year>1995</Year></PubDate></JournalIssue><Title>Archives of virology</Title><ISOAbbreviation>Arch. Virol.</ISOAbbreviation></Journal><ArticleTitle>A highly conserved epitope on the spike protein of infectious bronchitis virus.</ArticleTitle><Pagination><MedlinePgn>2201-13</MedlinePgn></Pagination><Abstract><AbstractText>The predicted amino acid sequence and secondary structures of S1 of the spike protein (S) of infectious bronchitis viral (IBV) strains from Europe, the U.S.A., and Japan were compared. An antigenic determinant that was highly conserved in both the primary amino acid sequence and secondary structure of all strains was identified between amino acid positions 240 to 255. A synthesized peptide corresponding to this region was found to react with all polyclonal antisera examined from various IBV strains and with one monoclonal antibody (MAb), 9B1B6, out of nine known to react with the S of Gray. The specificity of the interaction with MAb 9B1B6 was confirmed by competitive ELISA using bound and unbound peptide. Interestingly, the previously described epitope for 9B1B6 had been characterized as cross-reactive with several strains of IBV, as conformation-independent but reacting only with intact whole S, and as associated with the functional integrity of other epitopes, including neutralizing epitopes on the S protein. The apparent critical functional and structural nature of this highly immunogenic determinant suggests a potential contribution in developing protective, cross-reactive subunit vaccines to IBV.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Veterinary Pathobiology, Texas A&M University, College Station, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Parr</LastName><ForeName>R L</ForeName><Initials>RL</Initials></Author><Author ValidYN="Y"><LastName>King</LastName><ForeName>D J</ForeName><Initials>DJ</Initials></Author><Author ValidYN="Y"><LastName>Collisson</LastName><ForeName>E W</ForeName><Initials>EW</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Arch Virol</MedlineTA><NlmUniqueID>7506870</NlmUniqueID><ISSNLinking>0304-8608</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006389">Hemagglutinins, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003429" MajorTopicYN="N">Cross Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005060" MajorTopicYN="N" Type="Geographic">Europe</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006389" MajorTopicYN="N">Hemagglutinins, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015151" MajorTopicYN="N">Immunoblotting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001351" MajorTopicYN="N">Infectious bronchitis 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PubStatus="entrez"><Year>1995</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8572941</ArticleId><ArticleId IdType="doi">10.1007/bf01323240</ArticleId><ArticleId IdType="pmc">PMC7087072</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Virol. 1985 Nov;56(2):449-56</Citation><ArticleIdList><ArticleId IdType="pubmed">2997467</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1993 Feb;192(2):710-6</Citation><ArticleIdList><ArticleId IdType="pubmed">8380672</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1985 Sep;55(3):836-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2991600</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1981 Mar;53(Pt 1):93-103</Citation><ArticleIdList><ArticleId IdType="pubmed">6268743</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Dis. 1974 Apr-Jun;18(2):231-9</Citation><ArticleIdList><ArticleId IdType="pubmed">4597565</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 1993;133(3-4):369-83</Citation><ArticleIdList><ArticleId IdType="pubmed">7504916</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1987 Feb;156(2):331-41</Citation><ArticleIdList><ArticleId IdType="pubmed">3027982</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1988 Aug;165(2):589-95</Citation><ArticleIdList><ArticleId IdType="pubmed">2841803</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1984 Dec;65 ( Pt 12):2281-6</Citation><ArticleIdList><ArticleId IdType="pubmed">6096499</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 1979;61(3):227-38</Citation><ArticleIdList><ArticleId IdType="pubmed">227344</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Vet Res. 1951 Apr;12(43):140-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14829767</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 1988 Sep;11(2):141-50</Citation><ArticleIdList><ArticleId IdType="pubmed">2462314</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Pathol. 1992 Sep;21(3):401-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18670955</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 1994;138(1-2):117-34</Citation><ArticleIdList><ArticleId IdType="pubmed">7980002</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1986 Jul;67 ( Pt 7):1435-42</Citation><ArticleIdList><ArticleId IdType="pubmed">3014053</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1986 Mar;57(3):729-37</Citation><ArticleIdList><ArticleId IdType="pubmed">3005623</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Dis. 1969 May;13(2):356-62</Citation><ArticleIdList><ArticleId IdType="pubmed">5816048</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Dis. 1973 Jul-Sep;17(3):518-23</Citation><ArticleIdList><ArticleId IdType="pubmed">4748341</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 1986 Aug;5(2-3):253-63</Citation><ArticleIdList><ArticleId IdType="pubmed">2429473</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Q. 1984 Jul;6(3):114-20</Citation><ArticleIdList><ArticleId IdType="pubmed">6091319</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1991 Dec;72 ( Pt 12):2915-22</Citation><ArticleIdList><ArticleId IdType="pubmed">1722501</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Vet Res. 1962 Nov;23:1273-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14001258</ArticleId></ArticleIdList></Reference><Reference><Citation>Vaccine. 1990 Dec;8(6):605-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1708184</ArticleId></ArticleIdList></Reference><Reference><Citation>Viral Immunol. 1989 Fall;2(3):221-7</Citation><ArticleIdList><ArticleId IdType="pubmed">2560918</ArticleId></ArticleIdList></Reference><Reference><Citation>Avian Pathol. 1984 Jul;13(3):573-83</Citation><ArticleIdList><ArticleId IdType="pubmed">18766869</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8</Citation><ArticleIdList><ArticleId IdType="pubmed">6167991</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Rec. 1983 Jan 29;112(5):104-5</Citation><ArticleIdList><ArticleId IdType="pubmed">6298986</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1974 Jan 15;13(2):222-45</Citation><ArticleIdList><ArticleId IdType="pubmed">4358940</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1989 Mar;169(1):217-21</Citation><ArticleIdList><ArticleId IdType="pubmed">2466369</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 1994 Dec;34(3):327-38</Citation><ArticleIdList><ArticleId IdType="pubmed">7856318</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Vet Res. 1958 Jul;19(72):740-3</Citation><ArticleIdList><ArticleId IdType="pubmed">13559632</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 1995;140(2):259-71</Citation><ArticleIdList><ArticleId IdType="pubmed">7710354</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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