Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E.
Identifieur interne : 003450 ( PubMed/Corpus ); précédent : 003449; suivant : 003451Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E.
Auteurs : Aurelio Bonavia ; Bruce D. Zelus ; David E. Wentworth ; Pierre J. Talbot ; Kathryn V. HolmesSource :
- Journal of virology [ 0022-538X ] ; 2003.
English descriptors
- KwdEn :
- 3T3 Cells, Aminopeptidases (metabolism), Animals, Baculoviridae (genetics), Binding Sites, Cells, Cultured, Coronavirus 229E, Human (metabolism), Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (isolation & purification), Membrane Glycoproteins (metabolism), Mice, Receptors, Virus (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Spike Glycoprotein, Coronavirus, Spodoptera, Structure-Activity Relationship, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (isolation & purification), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , isolation & purification : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Aminopeptidases, Membrane Glycoproteins, Receptors, Virus, Recombinant Proteins, Viral Envelope Proteins.
- genetics : Baculoviridae.
- metabolism : Coronavirus 229E, Human.
- 3T3 Cells, Animals, Binding Sites, Cells, Cultured, Humans, Mice, Spike Glycoprotein, Coronavirus, Spodoptera, Structure-Activity Relationship.
Abstract
Human coronavirus HCoV-229E uses human aminopeptidase N (hAPN) as its receptor (C. L. Yeager et al., Nature 357:420-422, 1992). To identify the receptor-binding domain of the viral spike glycoprotein (S), we expressed soluble truncated histidine-tagged S glycoproteins by using baculovirus expression vectors. Truncated S proteins purified by nickel affinity chromatography were shown to be glycosylated and to react with polyclonal anti-HCoV-229E antibodies and monoclonal antibodies to the viral S protein. A truncated protein (S(547)) that contains the N-terminal 547 amino acids bound to 3T3 mouse cells that express hAPN but not to mouse 3T3 cells transfected with empty vector. Binding of S(547) to hAPN was blocked by an anti-hAPN monoclonal antibody that inhibits binding of virus to hAPN and blocks virus infection of human cells and was also blocked by polyclonal anti-HCoV-229E antibody. S proteins that contain the N-terminal 268 or 417 amino acids did not bind to hAPN-3T3 cells. Antibody to the region from amino acid 417 to the C terminus of S blocked binding of S(547) to hAPN-3T3 cells. Thus, the data suggest that the domain of the spike protein between amino acids 417 and 547 is required for the binding of HCoV-229E to its hAPN receptor.
DOI: 10.1128/jvi.77.4.2530-2538.2003
PubMed: 12551991
Links to Exploration step
pubmed:12551991Le document en format XML
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Talbot</name></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12551991</idno><idno type="pmid">12551991</idno><idno type="doi">10.1128/jvi.77.4.2530-2538.2003</idno><idno type="wicri:Area/PubMed/Corpus">003450</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003450</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E.</title><author><name sortKey="Bonavia, Aurelio" sort="Bonavia, Aurelio" uniqKey="Bonavia A" first="Aurelio" last="Bonavia">Aurelio Bonavia</name><affiliation><nlm:affiliation>Department of Microbiology Molecular Biology Program, University of Colorado Health Sciences Center, 4200 E 9th Avenue, Denver, CO 80262, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zelus, Bruce D" sort="Zelus, Bruce D" uniqKey="Zelus B" first="Bruce D" last="Zelus">Bruce D. Zelus</name></author><author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E" last="Wentworth">David E. Wentworth</name></author><author><name sortKey="Talbot, Pierre J" sort="Talbot, Pierre J" uniqKey="Talbot P" first="Pierre J" last="Talbot">Pierre J. Talbot</name></author><author><name sortKey="Holmes, Kathryn V" sort="Holmes, Kathryn V" uniqKey="Holmes K" first="Kathryn V" last="Holmes">Kathryn V. Holmes</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3T3 Cells</term><term>Aminopeptidases (metabolism)</term><term>Animals</term><term>Baculoviridae (genetics)</term><term>Binding Sites</term><term>Cells, Cultured</term><term>Coronavirus 229E, Human (metabolism)</term><term>Humans</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (isolation & purification)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Receptors, Virus (metabolism)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spodoptera</term><term>Structure-Activity Relationship</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aminopeptidases</term><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Baculoviridae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus 229E, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>3T3 Cells</term><term>Animals</term><term>Binding Sites</term><term>Cells, Cultured</term><term>Humans</term><term>Mice</term><term>Spike Glycoprotein, Coronavirus</term><term>Spodoptera</term><term>Structure-Activity Relationship</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human coronavirus HCoV-229E uses human aminopeptidase N (hAPN) as its receptor (C. L. Yeager et al., Nature 357:420-422, 1992). To identify the receptor-binding domain of the viral spike glycoprotein (S), we expressed soluble truncated histidine-tagged S glycoproteins by using baculovirus expression vectors. Truncated S proteins purified by nickel affinity chromatography were shown to be glycosylated and to react with polyclonal anti-HCoV-229E antibodies and monoclonal antibodies to the viral S protein. A truncated protein (S(547)) that contains the N-terminal 547 amino acids bound to 3T3 mouse cells that express hAPN but not to mouse 3T3 cells transfected with empty vector. Binding of S(547) to hAPN was blocked by an anti-hAPN monoclonal antibody that inhibits binding of virus to hAPN and blocks virus infection of human cells and was also blocked by polyclonal anti-HCoV-229E antibody. S proteins that contain the N-terminal 268 or 417 amino acids did not bind to hAPN-3T3 cells. Antibody to the region from amino acid 417 to the C terminus of S blocked binding of S(547) to hAPN-3T3 cells. Thus, the data suggest that the domain of the spike protein between amino acids 417 and 547 is required for the binding of HCoV-229E to its hAPN receptor.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12551991</PMID><DateCompleted><Year>2003</Year><Month>03</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>77</Volume><Issue>4</Issue><PubDate><Year>2003</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E.</ArticleTitle><Pagination><MedlinePgn>2530-8</MedlinePgn></Pagination><Abstract><AbstractText>Human coronavirus HCoV-229E uses human aminopeptidase N (hAPN) as its receptor (C. L. Yeager et al., Nature 357:420-422, 1992). To identify the receptor-binding domain of the viral spike glycoprotein (S), we expressed soluble truncated histidine-tagged S glycoproteins by using baculovirus expression vectors. Truncated S proteins purified by nickel affinity chromatography were shown to be glycosylated and to react with polyclonal anti-HCoV-229E antibodies and monoclonal antibodies to the viral S protein. A truncated protein (S(547)) that contains the N-terminal 547 amino acids bound to 3T3 mouse cells that express hAPN but not to mouse 3T3 cells transfected with empty vector. Binding of S(547) to hAPN was blocked by an anti-hAPN monoclonal antibody that inhibits binding of virus to hAPN and blocks virus infection of human cells and was also blocked by polyclonal anti-HCoV-229E antibody. S proteins that contain the N-terminal 268 or 417 amino acids did not bind to hAPN-3T3 cells. Antibody to the region from amino acid 417 to the C terminus of S blocked binding of S(547) to hAPN-3T3 cells. 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PubStatus="pubmed"><Year>2003</Year><Month>1</Month><Day>29</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>3</Month><Day>15</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>1</Month><Day>29</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12551991</ArticleId><ArticleId IdType="pmc">PMC141070</ArticleId><ArticleId IdType="doi">10.1128/jvi.77.4.2530-2538.2003</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Br Med J. 1968 Mar 9;1(5592):606-10</Citation><ArticleIdList><ArticleId IdType="pubmed">4295363</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2662-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10077567</ArticleId></ArticleIdList></Reference><Reference><Citation>Postgrad Med J. 1979 Feb;55(640):117-21</Citation><ArticleIdList><ArticleId IdType="pubmed">223137</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1981 Jan 29;289(5796):366-73</Citation><ArticleIdList><ArticleId IdType="pubmed">7464906</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 1981 Mar;31(3):1214-22</Citation><ArticleIdList><ArticleId IdType="pubmed">7228403</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Biol. 1981 Jun;89(3):674-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6265470</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1983 Jun;80(11):3153-7</Citation><ArticleIdList><ArticleId IdType="pubmed">6574476</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1985 Dec;56(3):912-20</Citation><ArticleIdList><ArticleId IdType="pubmed">2999444</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Biochem. 1987;56:365-94</Citation><ArticleIdList><ArticleId IdType="pubmed">3304138</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1987 Dec;161(2):479-87</Citation><ArticleIdList><ArticleId IdType="pubmed">2825419</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1990 Feb;71 ( Pt 2):271-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2155284</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Immunol. 1990;8:737-71</Citation><ArticleIdList><ArticleId IdType="pubmed">2188678</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1990 May;71 ( Pt 5):1065-73</Citation><ArticleIdList><ArticleId IdType="pubmed">2345367</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Top Microbiol Immunol. 1990;157:187-253</Citation><ArticleIdList><ArticleId IdType="pubmed">2203609</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1990 Nov;64(11):5367-75</Citation><ArticleIdList><ArticleId IdType="pubmed">2170676</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1991 Jul;183(1):225-38</Citation><ArticleIdList><ArticleId IdType="pubmed">1711257</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1991 Dec;65(12):6979-84</Citation><ArticleIdList><ArticleId IdType="pubmed">1719237</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1992 Jun 4;357(6377):417-20</Citation><ArticleIdList><ArticleId IdType="pubmed">1350661</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1992 Jun 4;357(6377):420-2</Citation><ArticleIdList><ArticleId IdType="pubmed">1350662</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1992 Sep;190(1):92-105</Citation><ArticleIdList><ArticleId IdType="pubmed">1326823</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1992 Nov 6;258(5084):917-24</Citation><ArticleIdList><ArticleId IdType="pubmed">1439803</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 1993;342:293-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7911642</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1994 Aug;68(8):5216-24</Citation><ArticleIdList><ArticleId IdType="pubmed">7913510</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1994 Dec;68(12):8008-16</Citation><ArticleIdList><ArticleId IdType="pubmed">7525985</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1995 Mar;69(3):1435-43</Citation><ArticleIdList><ArticleId IdType="pubmed">7853475</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 1995;380:367-70</Citation><ArticleIdList><ArticleId IdType="pubmed">8830509</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1996 Apr;70(4):2632-6</Citation><ArticleIdList><ArticleId IdType="pubmed">8642698</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Vet Res. 1996 May;57(5):664-71</Citation><ArticleIdList><ArticleId IdType="pubmed">8723879</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1996 Oct;77 ( Pt 10):2515-21</Citation><ArticleIdList><ArticleId IdType="pubmed">8887485</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1997 Jan;71(1):734-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8985407</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1997 Apr;71(4):3129-37</Citation><ArticleIdList><ArticleId IdType="pubmed">9060676</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1997 Mar 17;229(2):336-48</Citation><ArticleIdList><ArticleId IdType="pubmed">9126247</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 1999 Mar;3(3):309-19</Citation><ArticleIdList><ArticleId IdType="pubmed">10198633</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1999 Sep;73(9):7607-18</Citation><ArticleIdList><ArticleId IdType="pubmed">10438851</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2000 Feb;81(Pt 2):489-96</Citation><ArticleIdList><ArticleId IdType="pubmed">10644848</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2000 May;81(Pt 5):1383-91</Citation><ArticleIdList><ArticleId IdType="pubmed">10769082</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2000 Oct;74(19):9234-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10982370</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2001 Mar;75(6):2792-802</Citation><ArticleIdList><ArticleId IdType="pubmed">11222703</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Cell Res. 1999 Nov 1;252(2):243-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11501563</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2001 Oct;75(20):9741-52</Citation><ArticleIdList><ArticleId IdType="pubmed">11559807</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2002 May 1;21(9):2076-86</Citation><ArticleIdList><ArticleId IdType="pubmed">11980704</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2003 Jan;77(2):830-40</Citation><ArticleIdList><ArticleId IdType="pubmed">12502799</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2003 Jan;77(2):841-50</Citation><ArticleIdList><ArticleId IdType="pubmed">12502800</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2003 Apr;77(7):4435-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12634402</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Soc Exp Biol Med. 1966 Jan;121(1):190-3</Citation><ArticleIdList><ArticleId IdType="pubmed">4285768</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1997 Nov;71(11):8357-61</Citation><ArticleIdList><ArticleId IdType="pubmed">9343190</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1997 Nov;78 ( Pt 11):2795-802</Citation><ArticleIdList><ArticleId IdType="pubmed">9367365</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1997 Dec;71(12):9427-33</Citation><ArticleIdList><ArticleId IdType="pubmed">9371603</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Jan;72(1):527-34</Citation><ArticleIdList><ArticleId IdType="pubmed">9420255</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Mar;72(3):1941-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9499047</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1998 Jun;79 ( Pt 6):1387-91</Citation><ArticleIdList><ArticleId IdType="pubmed">9634079</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1998 Jun 19;280(5371):1884-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9632381</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1998 Jun 19;280(5371):1949-53</Citation><ArticleIdList><ArticleId IdType="pubmed">9632396</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1998 Jun 18;393(6686):705-11</Citation><ArticleIdList><ArticleId IdType="pubmed">9641684</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Sep;72(9):7237-44</Citation><ArticleIdList><ArticleId IdType="pubmed">9696818</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 1998;440:17-23</Citation><ArticleIdList><ArticleId IdType="pubmed">9782260</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 1998;440:61-7</Citation><ArticleIdList><ArticleId IdType="pubmed">9782265</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 1998;440:69-75</Citation><ArticleIdList><ArticleId IdType="pubmed">9782266</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 1969 May;102(5):1109-18</Citation><ArticleIdList><ArticleId IdType="pubmed">4306070</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:12551991" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
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