Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
Identifieur interne : 003343 ( PubMed/Corpus ); précédent : 003342; suivant : 003344Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
Auteurs : Qin-Fen Zhang ; Jin-Ming Cui ; Xiao-Jun Huang ; Wei Lin ; Dong-Yan Tan ; Jie-Wei Xu ; Yi-Feng Yang ; Jing-Qiang Zhang ; Xin Zhang ; Hui Li ; Huan-Ying Zheng ; Qiu-Xia Chen ; Xin-Ge Yan ; Kui Zheng ; Zhuo-Yue Wan ; Jie-Cheng HuangSource :
- Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [ 0582-9879 ] ; 2003.
English descriptors
- KwdEn :
- Animals, Cell Nucleus (virology), Chlorocebus aethiops, Humans, Larynx (virology), Microscopy, Electron, Nasopharynx (virology), Nuclear Envelope (virology), SARS Virus (immunology), SARS Virus (isolation & purification), SARS Virus (ultrastructure), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Vero Cells (virology), Virion (ultrastructure).
- MESH :
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- ultrastructure : SARS Virus, Virion.
- virology : Cell Nucleus, Larynx, Nasopharynx, Nuclear Envelope, Severe Acute Respiratory Syndrome, Vero Cells.
- Animals, Chlorocebus aethiops, Humans, Microscopy, Electron.
Abstract
After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.
PubMed: 12796822
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Jie Wei" uniqKey="Xu J" first="Jie-Wei" last="Xu">Jie-Wei Xu</name></author><author><name sortKey="Yang, Yi Feng" sort="Yang, Yi Feng" uniqKey="Yang Y" first="Yi-Feng" last="Yang">Yi-Feng Yang</name></author><author><name sortKey="Zhang, Jing Qiang" sort="Zhang, Jing Qiang" uniqKey="Zhang J" first="Jing-Qiang" last="Zhang">Jing-Qiang Zhang</name></author><author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name></author><author><name sortKey="Li, Hui" sort="Li, Hui" uniqKey="Li H" first="Hui" last="Li">Hui Li</name></author><author><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name></author><author><name sortKey="Chen, Qiu Xia" sort="Chen, Qiu Xia" uniqKey="Chen Q" first="Qiu-Xia" last="Chen">Qiu-Xia Chen</name></author><author><name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name></author><author><name sortKey="Zheng, Kui" sort="Zheng, Kui" uniqKey="Zheng K" first="Kui" last="Zheng">Kui Zheng</name></author><author><name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name></author><author><name sortKey="Huang, Jie Cheng" sort="Huang, Jie Cheng" uniqKey="Huang J" first="Jie-Cheng" last="Huang">Jie-Cheng Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12796822</idno><idno type="pmid">12796822</idno><idno type="wicri:Area/PubMed/Corpus">003343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003343</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.</title><author><name sortKey="Zhang, Qin Fen" sort="Zhang, Qin Fen" uniqKey="Zhang Q" first="Qin-Fen" last="Zhang">Qin-Fen Zhang</name><affiliation><nlm:affiliation>State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.</nlm:affiliation></affiliation></author><author><name sortKey="Cui, Jin Ming" sort="Cui, Jin Ming" uniqKey="Cui J" first="Jin-Ming" last="Cui">Jin-Ming Cui</name></author><author><name sortKey="Huang, Xiao Jun" sort="Huang, Xiao Jun" uniqKey="Huang X" first="Xiao-Jun" last="Huang">Xiao-Jun Huang</name></author><author><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name></author><author><name sortKey="Tan, Dong Yan" sort="Tan, Dong Yan" uniqKey="Tan D" first="Dong-Yan" last="Tan">Dong-Yan Tan</name></author><author><name sortKey="Xu, Jie Wei" sort="Xu, Jie Wei" uniqKey="Xu J" first="Jie-Wei" last="Xu">Jie-Wei Xu</name></author><author><name sortKey="Yang, Yi Feng" sort="Yang, Yi Feng" uniqKey="Yang Y" first="Yi-Feng" last="Yang">Yi-Feng Yang</name></author><author><name sortKey="Zhang, Jing Qiang" sort="Zhang, Jing Qiang" uniqKey="Zhang J" first="Jing-Qiang" last="Zhang">Jing-Qiang Zhang</name></author><author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name></author><author><name sortKey="Li, Hui" sort="Li, Hui" uniqKey="Li H" first="Hui" last="Li">Hui Li</name></author><author><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name></author><author><name sortKey="Chen, Qiu Xia" sort="Chen, Qiu Xia" uniqKey="Chen Q" first="Qiu-Xia" last="Chen">Qiu-Xia Chen</name></author><author><name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name></author><author><name sortKey="Zheng, Kui" sort="Zheng, Kui" uniqKey="Zheng K" first="Kui" last="Zheng">Kui Zheng</name></author><author><name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name></author><author><name sortKey="Huang, Jie Cheng" sort="Huang, Jie Cheng" uniqKey="Huang J" first="Jie-Cheng" last="Huang">Jie-Cheng Huang</name></author></analytic><series><title level="j">Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica</title><idno type="ISSN">0582-9879</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Nucleus (virology)</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Larynx (virology)</term><term>Microscopy, Electron</term><term>Nasopharynx (virology)</term><term>Nuclear Envelope (virology)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (ultrastructure)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vero Cells (virology)</term><term>Virion (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Nucleus</term><term>Larynx</term><term>Nasopharynx</term><term>Nuclear Envelope</term><term>Severe Acute Respiratory Syndrome</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Microscopy, Electron</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12796822</PMID><DateCompleted><Year>2005</Year><Month>04</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0582-9879</ISSN><JournalIssue CitedMedium="Print"><Volume>35</Volume><Issue>6</Issue><PubDate><Year>2003</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica</Title><ISOAbbreviation>Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao</ISOAbbreviation></Journal><ArticleTitle>Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.</ArticleTitle><Pagination><MedlinePgn>587-91</MedlinePgn></Pagination><Abstract><AbstractText>After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Qin-Fen</ForeName><Initials>QF</Initials><AffiliationInfo><Affiliation>State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cui</LastName><ForeName>Jin-Ming</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xiao-Jun</ForeName><Initials>XJ</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Dong-Yan</ForeName><Initials>DY</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jie-Wei</ForeName><Initials>JW</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yi-Feng</ForeName><Initials>YF</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jing-Qiang</ForeName><Initials>JQ</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hui</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Huan-Ying</ForeName><Initials>HY</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Qiu-Xia</ForeName><Initials>QX</Initials></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Xin-Ge</ForeName><Initials>XG</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Kui</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Wan</LastName><ForeName>Zhuo-Yue</ForeName><Initials>ZY</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Jie-Cheng</ForeName><Initials>JC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)</MedlineTA><NlmUniqueID>20730160R</NlmUniqueID><ISSNLinking>0582-9879</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007830" MajorTopicYN="N">Larynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008854" MajorTopicYN="N">Microscopy, Electron</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009685" MajorTopicYN="N">Nuclear Envelope</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014771" MajorTopicYN="N">Virion</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>6</Month><Day>11</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>4</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>6</Month><Day>11</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12796822</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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