[The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome].
Identifieur interne : 003284 ( PubMed/Corpus ); précédent : 003283; suivant : 003285[The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome].
Auteurs : Li-Qun Fang ; Pan-He Zhang ; Bao-An Yang ; Xiao-Ming Wu ; Qiu-Min Zhao ; Wei Liu ; Hong Liu ; Yong-Qiang Deng ; Lin Zhan ; Wei-Guo Han ; Fu-Shuang Lu ; Jin-Song Wu ; Hong Yang ; Qing-Yu Zhu ; Wu-Chun CaoSource :
- Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi [ 0254-6450 ] ; 2003.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G, Immunoglobulin M.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- methods : Fluorescent Antibody Technique, Indirect.
- Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.
PubMed: 12848915
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome].</title><author><name sortKey="Fang, Li Qun" sort="Fang, Li Qun" uniqKey="Fang L" first="Li-Qun" last="Fang">Li-Qun Fang</name><affiliation><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Pan He" sort="Zhang, Pan He" uniqKey="Zhang P" first="Pan-He" last="Zhang">Pan-He Zhang</name></author><author><name sortKey="Yang, Bao An" sort="Yang, Bao An" uniqKey="Yang B" first="Bao-An" last="Yang">Bao-An Yang</name></author><author><name sortKey="Wu, Xiao Ming" sort="Wu, Xiao Ming" uniqKey="Wu X" first="Xiao-Ming" last="Wu">Xiao-Ming Wu</name></author><author><name sortKey="Zhao, Qiu Min" sort="Zhao, Qiu Min" uniqKey="Zhao Q" first="Qiu-Min" last="Zhao">Qiu-Min Zhao</name></author><author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name></author><author><name sortKey="Liu, Hong" sort="Liu, Hong" uniqKey="Liu H" first="Hong" last="Liu">Hong Liu</name></author><author><name sortKey="Deng, Yong Qiang" sort="Deng, Yong Qiang" uniqKey="Deng Y" first="Yong-Qiang" last="Deng">Yong-Qiang Deng</name></author><author><name sortKey="Zhan, Lin" sort="Zhan, Lin" uniqKey="Zhan L" first="Lin" last="Zhan">Lin Zhan</name></author><author><name sortKey="Han, Wei Guo" sort="Han, Wei Guo" uniqKey="Han W" first="Wei-Guo" last="Han">Wei-Guo Han</name></author><author><name sortKey="Lu, Fu Shuang" sort="Lu, Fu Shuang" uniqKey="Lu F" first="Fu-Shuang" last="Lu">Fu-Shuang Lu</name></author><author><name sortKey="Wu, Jin Song" sort="Wu, Jin Song" uniqKey="Wu J" first="Jin-Song" last="Wu">Jin-Song Wu</name></author><author><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name></author><author><name sortKey="Zhu, Qing Yu" sort="Zhu, Qing Yu" uniqKey="Zhu Q" first="Qing-Yu" last="Zhu">Qing-Yu Zhu</name></author><author><name sortKey="Cao, Wu Chun" sort="Cao, Wu Chun" uniqKey="Cao W" first="Wu-Chun" last="Cao">Wu-Chun Cao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12848915</idno><idno type="pmid">12848915</idno><idno type="wicri:Area/PubMed/Corpus">003284</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003284</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome].</title><author><name sortKey="Fang, Li Qun" sort="Fang, Li Qun" uniqKey="Fang L" first="Li-Qun" last="Fang">Li-Qun Fang</name><affiliation><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Pan He" sort="Zhang, Pan He" uniqKey="Zhang P" first="Pan-He" last="Zhang">Pan-He Zhang</name></author><author><name sortKey="Yang, Bao An" sort="Yang, Bao An" uniqKey="Yang B" first="Bao-An" last="Yang">Bao-An Yang</name></author><author><name sortKey="Wu, Xiao Ming" sort="Wu, Xiao Ming" uniqKey="Wu X" first="Xiao-Ming" last="Wu">Xiao-Ming Wu</name></author><author><name sortKey="Zhao, Qiu Min" sort="Zhao, Qiu Min" uniqKey="Zhao Q" first="Qiu-Min" last="Zhao">Qiu-Min Zhao</name></author><author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name></author><author><name sortKey="Liu, Hong" sort="Liu, Hong" uniqKey="Liu H" first="Hong" last="Liu">Hong Liu</name></author><author><name sortKey="Deng, Yong Qiang" sort="Deng, Yong Qiang" uniqKey="Deng Y" first="Yong-Qiang" last="Deng">Yong-Qiang Deng</name></author><author><name sortKey="Zhan, Lin" sort="Zhan, Lin" uniqKey="Zhan L" first="Lin" last="Zhan">Lin Zhan</name></author><author><name sortKey="Han, Wei Guo" sort="Han, Wei Guo" uniqKey="Han W" first="Wei-Guo" last="Han">Wei-Guo Han</name></author><author><name sortKey="Lu, Fu Shuang" sort="Lu, Fu Shuang" uniqKey="Lu F" first="Fu-Shuang" last="Lu">Fu-Shuang Lu</name></author><author><name sortKey="Wu, Jin Song" sort="Wu, Jin Song" uniqKey="Wu J" first="Jin-Song" last="Wu">Jin-Song Wu</name></author><author><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name></author><author><name sortKey="Zhu, Qing Yu" sort="Zhu, Qing Yu" uniqKey="Zhu Q" first="Qing-Yu" last="Zhu">Qing-Yu Zhu</name></author><author><name sortKey="Cao, Wu Chun" sort="Cao, Wu Chun" uniqKey="Cao W" first="Wu-Chun" last="Cao">Wu-Chun Cao</name></author></analytic><series><title level="j">Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi</title><idno type="ISSN">0254-6450</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique, Indirect (methods)</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Immunoglobulin M (blood)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Fluorescent Antibody Technique, Indirect</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12848915</PMID><DateCompleted><Year>2003</Year><Month>12</Month><Day>18</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0254-6450</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>6</Issue><PubDate><Year>2003</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi</Title><ISOAbbreviation>Zhonghua Liu Xing Bing Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome].</ArticleTitle><Pagination><MedlinePgn>484-6</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Li-qun</ForeName><Initials>LQ</Initials><AffiliationInfo><Affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Pan-he</ForeName><Initials>PH</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Bao-an</ForeName><Initials>BA</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiao-ming</ForeName><Initials>XM</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Qiu-min</ForeName><Initials>QM</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Yong-qiang</ForeName><Initials>YQ</Initials></Author><Author ValidYN="Y"><LastName>Zhan</LastName><ForeName>Lin</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Wei-guo</ForeName><Initials>WG</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Fu-shuang</ForeName><Initials>FS</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jin-song</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Qing-yu</ForeName><Initials>QY</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Wu-chun</ForeName><Initials>WC</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Liu Xing Bing Xue Za Zhi</MedlineTA><NlmUniqueID>8208604</NlmUniqueID><ISSNLinking>0254-6450</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007075">Immunoglobulin M</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019084" MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007075" MajorTopicYN="N">Immunoglobulin M</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>7</Month><Day>10</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>12</Month><Day>19</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>7</Month><Day>10</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12848915</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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