SrasV1, PubMed, Corpus, bibRecord, 003238

[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].

Identifieur interne : 003238 ( PubMed/Corpus ); précédent : 003237; suivant : 003239

[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].

Auteurs : Yi Ren ; Hui-Guo Ding ; Qing-Fa Wu ; Wei-Jun Chen ; Dong Chen ; Zhi-Ying Bao ; Ling Yang ; Chun-Hui Zhao ; Jian Wang

Source :

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [ 1000-503X ] ; 2003.

RBID : pubmed:12905760

English descriptors

KwdEn :
- Adolescent, Adult, Feces (virology), Female, Humans, Male, Middle Aged, RNA, Viral (analysis), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Time Factors.
MESH :
- chemical , analysis : RNA, Viral.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- transmission : Severe Acute Respiratory Syndrome.
- virology : Feces, Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Female, Humans, Male, Middle Aged, Time Factors.

Abstract

To investigate of severe acute respiratory syndrome (SARS) convalescent stool shedding by RT-PCR.

PubMed: 12905760

Links to Exploration step

pubmed:12905760

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].</title>
<author><name sortKey="Ren, Yi" sort="Ren, Yi" uniqKey="Ren Y" first="Yi" last="Ren">Yi Ren</name>
<affiliation><nlm:affiliation>Department of Infectious Diseases, Beijing You'an Hospital, Capital University of Medicine Science, Beijing 100054, China. renyi@staff.healthoo.com</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ding, Hui Guo" sort="Ding, Hui Guo" uniqKey="Ding H" first="Hui-Guo" last="Ding">Hui-Guo Ding</name>
</author>
<author><name sortKey="Wu, Qing Fa" sort="Wu, Qing Fa" uniqKey="Wu Q" first="Qing-Fa" last="Wu">Qing-Fa Wu</name>
</author>
<author><name sortKey="Chen, Wei Jun" sort="Chen, Wei Jun" uniqKey="Chen W" first="Wei-Jun" last="Chen">Wei-Jun Chen</name>
</author>
<author><name sortKey="Chen, Dong" sort="Chen, Dong" uniqKey="Chen D" first="Dong" last="Chen">Dong Chen</name>
</author>
<author><name sortKey="Bao, Zhi Ying" sort="Bao, Zhi Ying" uniqKey="Bao Z" first="Zhi-Ying" last="Bao">Zhi-Ying Bao</name>
</author>
<author><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name>
</author>
<author><name sortKey="Zhao, Chun Hui" sort="Zhao, Chun Hui" uniqKey="Zhao C" first="Chun-Hui" last="Zhao">Chun-Hui Zhao</name>
</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2003">2003</date>
<idno type="RBID">pubmed:12905760</idno>
<idno type="pmid">12905760</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].</title>
<author><name sortKey="Ren, Yi" sort="Ren, Yi" uniqKey="Ren Y" first="Yi" last="Ren">Yi Ren</name>
<affiliation><nlm:affiliation>Department of Infectious Diseases, Beijing You'an Hospital, Capital University of Medicine Science, Beijing 100054, China. renyi@staff.healthoo.com</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ding, Hui Guo" sort="Ding, Hui Guo" uniqKey="Ding H" first="Hui-Guo" last="Ding">Hui-Guo Ding</name>
</author>
<author><name sortKey="Wu, Qing Fa" sort="Wu, Qing Fa" uniqKey="Wu Q" first="Qing-Fa" last="Wu">Qing-Fa Wu</name>
</author>
<author><name sortKey="Chen, Wei Jun" sort="Chen, Wei Jun" uniqKey="Chen W" first="Wei-Jun" last="Chen">Wei-Jun Chen</name>
</author>
<author><name sortKey="Chen, Dong" sort="Chen, Dong" uniqKey="Chen D" first="Dong" last="Chen">Dong Chen</name>
</author>
<author><name sortKey="Bao, Zhi Ying" sort="Bao, Zhi Ying" uniqKey="Bao Z" first="Zhi-Ying" last="Bao">Zhi-Ying Bao</name>
</author>
<author><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name>
</author>
<author><name sortKey="Zhao, Chun Hui" sort="Zhao, Chun Hui" uniqKey="Zhao C" first="Chun-Hui" last="Zhao">Chun-Hui Zhao</name>
</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
</author>
</analytic>
<series><title level="j">Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae</title>
<idno type="ISSN">1000-503X</idno>
<imprint><date when="2003" type="published">2003</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Feces (virology)</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>RNA, Viral (analysis)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Severe Acute Respiratory Syndrome (transmission)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Time Factors</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">To investigate of severe acute respiratory syndrome (SARS) convalescent stool shedding by RT-PCR.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12905760</PMID>
<DateCompleted><Year>2004</Year>
<Month>07</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-503X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>25</Volume>
<Issue>3</Issue>
<PubDate><Year>2003</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae</Title>
<ISOAbbreviation>Zhongguo Yi Xue Ke Xue Yuan Xue Bao</ISOAbbreviation>
</Journal>
<ArticleTitle>[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].</ArticleTitle>
<Pagination><MedlinePgn>368-71</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To investigate of severe acute respiratory syndrome (SARS) convalescent stool shedding by RT-PCR.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">One hundred and three stool samples from 46 SARS patients were collected on May 16th, 20th, and 23rd, 2003. For each sample, RNA was extracted using commercial kit and 7 Nest RT-PCR using a 14-pair different SARS-associated coronavirus (SARS-CoV) special primers were carried out simultaneously.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Among these 46 SARS patients, 17 cases (37.0%) were stool SARS-CoV RT-PCR negative, and 29 cases (63.0%) were SARS-CoV RT-PCR positive. The duration of positive cases lasted (31.76 +/- 10.78) d (12-64 d). The longest stool shedding case in this study lasted 64 days. Two serial stool samples and for each sample 2 RT-PCR tests using different primers were positive in this case.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Our study observed longest stool shedding of SARS patients to be 64 days after initial onset of SARS. The average stool shedding was 32 days. Hence it is important to think highly of SARS convalescent patient stool sterilization.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ren</LastName>
<ForeName>Yi</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Infectious Diseases, Beijing You'an Hospital, Capital University of Medicine Science, Beijing 100054, China. renyi@staff.healthoo.com</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ding</LastName>
<ForeName>Hui-guo</ForeName>
<Initials>HG</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Qing-fa</ForeName>
<Initials>QF</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Wei-jun</ForeName>
<Initials>WJ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Dong</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Bao</LastName>
<ForeName>Zhi-ying</ForeName>
<Initials>ZY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Ling</ForeName>
<Initials>L</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Chun-hui</ForeName>
<Initials>CH</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Jian</ForeName>
<Initials>J</Initials>
</Author>
</AuthorList>
<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhongguo Yi Xue Ke Xue Yuan Xue Bao</MedlineTA>
<NlmUniqueID>8006230</NlmUniqueID>
<ISSNLinking>1000-503X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005243" MajorTopicYN="N">Feces</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year>
<Month>8</Month>
<Day>9</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2004</Year>
<Month>7</Month>
<Day>16</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2003</Year>
<Month>8</Month>
<Day>9</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">12905760</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

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[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].

[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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