Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.
Identifieur interne : 002D48 ( PubMed/Corpus ); précédent : 002D47; suivant : 002D49Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.
Auteurs : Xiao-Yan Che ; Li-Wen Qiu ; Yu-Xian Pan ; Kun Wen ; Wei Hao ; Li-Ya Zhang ; Ya-Di Wang ; Zhi-Yong Liao ; Xu Hua ; Vincent C C. Cheng ; Kwok-Yung YuenSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2004.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Viral.
- chemical , immunology : Antibodies, Monoclonal.
- blood : Nucleocapsid.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Rabbits, Reproducibility of Results, Sensitivity and Specificity.
Abstract
A rapid antigen test for the diagnosis of severe acute respiratory syndrome (SARS) is essential for control of this disease at the point of management. The nucleocapsid (N) protein of SARS-associated coronavirus (SARS-CoV) is abundantly expressed in infected-cell culture filtrate as demonstrable by Western blotting using convalescent-phase sera from patients with SARS. We used monoclonal antibodies specifically directed against N protein to establish a sensitive antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of SARS-CoV. The assay employed a mixture of three monoclonal antibodies for capture and rabbit polyclonal antibodies for detection of serum antigen in 32 cases of clinically probable SARS as defined by the World Health Organization during the epidemic in Guangzhou, China. Recombinant N protein was used as a standard to establish a detection sensitivity of approximated 50 pg/ml. The linear range of detection in clinical specimens was from 100 pg/ml to 3.2 ng/ml. Using a panel of sera collected at different points in time, the amount of circulating N antigen was found to peak 6 to 10 days after the onset of symptoms. The sensitivity of the assay was 84.6% in 13 serologically confirmed SARS patients with blood taken during the first 10 days after the onset of symptoms (11 of 13). The specificity of the assay was 98.5% in 1,272 healthy individuals (1,253 of 1,272). There was no cross-reaction with other human and animal coronaviruses in this assay. In conclusion, a sensitive and quantitative antigen capture ELISA was established for the early diagnosis and disease monitoring of SARS-CoV infection.
DOI: 10.1128/JCM.42.6.2629-2635.2004
PubMed: 15184444
Links to Exploration step
pubmed:15184444Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.</title><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation><nlm:affiliation>Center of Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, P. R. China. linche@pub.guangzhou.gd.cn</nlm:affiliation></affiliation></author><author><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name></author><author><name sortKey="Pan, Yu Xian" sort="Pan, Yu Xian" uniqKey="Pan Y" first="Yu-Xian" last="Pan">Yu-Xian Pan</name></author><author><name sortKey="Wen, Kun" sort="Wen, Kun" uniqKey="Wen K" first="Kun" last="Wen">Kun Wen</name></author><author><name sortKey="Hao, Wei" sort="Hao, Wei" uniqKey="Hao W" first="Wei" last="Hao">Wei Hao</name></author><author><name sortKey="Zhang, Li Ya" sort="Zhang, Li Ya" uniqKey="Zhang L" first="Li-Ya" last="Zhang">Li-Ya Zhang</name></author><author><name sortKey="Wang, Ya Di" sort="Wang, Ya Di" uniqKey="Wang Y" first="Ya-Di" last="Wang">Ya-Di Wang</name></author><author><name sortKey="Liao, Zhi Yong" sort="Liao, Zhi Yong" uniqKey="Liao Z" first="Zhi-Yong" last="Liao">Zhi-Yong Liao</name></author><author><name sortKey="Hua, Xu" sort="Hua, Xu" uniqKey="Hua X" first="Xu" last="Hua">Xu Hua</name></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C C" last="Cheng">Vincent C C. Cheng</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15184444</idno><idno type="pmid">15184444</idno><idno type="doi">10.1128/JCM.42.6.2629-2635.2004</idno><idno type="wicri:Area/PubMed/Corpus">002D48</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D48</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.</title><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation><nlm:affiliation>Center of Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, P. R. China. linche@pub.guangzhou.gd.cn</nlm:affiliation></affiliation></author><author><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name></author><author><name sortKey="Pan, Yu Xian" sort="Pan, Yu Xian" uniqKey="Pan Y" first="Yu-Xian" last="Pan">Yu-Xian Pan</name></author><author><name sortKey="Wen, Kun" sort="Wen, Kun" uniqKey="Wen K" first="Kun" last="Wen">Kun Wen</name></author><author><name sortKey="Hao, Wei" sort="Hao, Wei" uniqKey="Hao W" first="Wei" last="Hao">Wei Hao</name></author><author><name sortKey="Zhang, Li Ya" sort="Zhang, Li Ya" uniqKey="Zhang L" first="Li-Ya" last="Zhang">Li-Ya Zhang</name></author><author><name sortKey="Wang, Ya Di" sort="Wang, Ya Di" uniqKey="Wang Y" first="Ya-Di" last="Wang">Ya-Di Wang</name></author><author><name sortKey="Liao, Zhi Yong" sort="Liao, Zhi Yong" uniqKey="Liao Z" first="Zhi-Yong" last="Liao">Zhi-Yong Liao</name></author><author><name sortKey="Hua, Xu" sort="Hua, Xu" uniqKey="Hua X" first="Xu" last="Hua">Xu Hua</name></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C C" last="Cheng">Vincent C C. Cheng</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Viral (blood)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Nucleocapsid (blood)</term><term>Rabbits</term><term>Reproducibility of Results</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Nucleocapsid</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Rabbits</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A rapid antigen test for the diagnosis of severe acute respiratory syndrome (SARS) is essential for control of this disease at the point of management. The nucleocapsid (N) protein of SARS-associated coronavirus (SARS-CoV) is abundantly expressed in infected-cell culture filtrate as demonstrable by Western blotting using convalescent-phase sera from patients with SARS. We used monoclonal antibodies specifically directed against N protein to establish a sensitive antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of SARS-CoV. The assay employed a mixture of three monoclonal antibodies for capture and rabbit polyclonal antibodies for detection of serum antigen in 32 cases of clinically probable SARS as defined by the World Health Organization during the epidemic in Guangzhou, China. Recombinant N protein was used as a standard to establish a detection sensitivity of approximated 50 pg/ml. The linear range of detection in clinical specimens was from 100 pg/ml to 3.2 ng/ml. Using a panel of sera collected at different points in time, the amount of circulating N antigen was found to peak 6 to 10 days after the onset of symptoms. The sensitivity of the assay was 84.6% in 13 serologically confirmed SARS patients with blood taken during the first 10 days after the onset of symptoms (11 of 13). The specificity of the assay was 98.5% in 1,272 healthy individuals (1,253 of 1,272). There was no cross-reaction with other human and animal coronaviruses in this assay. In conclusion, a sensitive and quantitative antigen capture ELISA was established for the early diagnosis and disease monitoring of SARS-CoV infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15184444</PMID><DateCompleted><Year>2004</Year><Month>07</Month><Day>29</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>6</Issue><PubDate><Year>2004</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.</ArticleTitle><Pagination><MedlinePgn>2629-35</MedlinePgn></Pagination><Abstract><AbstractText>A rapid antigen test for the diagnosis of severe acute respiratory syndrome (SARS) is essential for control of this disease at the point of management. The nucleocapsid (N) protein of SARS-associated coronavirus (SARS-CoV) is abundantly expressed in infected-cell culture filtrate as demonstrable by Western blotting using convalescent-phase sera from patients with SARS. We used monoclonal antibodies specifically directed against N protein to establish a sensitive antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of SARS-CoV. The assay employed a mixture of three monoclonal antibodies for capture and rabbit polyclonal antibodies for detection of serum antigen in 32 cases of clinically probable SARS as defined by the World Health Organization during the epidemic in Guangzhou, China. Recombinant N protein was used as a standard to establish a detection sensitivity of approximated 50 pg/ml. The linear range of detection in clinical specimens was from 100 pg/ml to 3.2 ng/ml. Using a panel of sera collected at different points in time, the amount of circulating N antigen was found to peak 6 to 10 days after the onset of symptoms. The sensitivity of the assay was 84.6% in 13 serologically confirmed SARS patients with blood taken during the first 10 days after the onset of symptoms (11 of 13). The specificity of the assay was 98.5% in 1,272 healthy individuals (1,253 of 1,272). There was no cross-reaction with other human and animal coronaviruses in this assay. In conclusion, a sensitive and quantitative antigen capture ELISA was established for the early diagnosis and disease monitoring of SARS-CoV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Che</LastName><ForeName>Xiao-Yan</ForeName><Initials>XY</Initials><AffiliationInfo><Affiliation>Center of Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, P. R. China. linche@pub.guangzhou.gd.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Li-Wen</ForeName><Initials>LW</Initials></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Yu-Xian</ForeName><Initials>YX</Initials></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Kun</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Hao</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Li-Ya</ForeName><Initials>LY</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ya-Di</ForeName><Initials>YD</Initials></Author><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Zhi-Yong</ForeName><Initials>ZY</Initials></Author><Author ValidYN="Y"><LastName>Hua</LastName><ForeName>Xu</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Vincent C C</ForeName><Initials>VC</Initials></Author><Author ValidYN="Y"><LastName>Yuen</LastName><ForeName>Kwok-Yung</ForeName><Initials>KY</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019251" MajorTopicYN="N">Nucleocapsid</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>6</Month><Day>9</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>7</Month><Day>30</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>6</Month><Day>9</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15184444</ArticleId><ArticleId IdType="doi">10.1128/JCM.42.6.2629-2635.2004</ArticleId><ArticleId IdType="pii">42/6/2629</ArticleId><ArticleId IdType="pmc">PMC427886</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Clin Microbiol. 2000 Apr;38(4):1430-3</Citation><ArticleIdList><ArticleId IdType="pubmed">10747120</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2003 Jan;41(1):135-43</Citation><ArticleIdList><ArticleId IdType="pubmed">12517838</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2002 Dec;40(12):4603-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12454159</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2003 Mar;41(3):1016-22</Citation><ArticleIdList><ArticleId IdType="pubmed">12624024</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 May 15;423(6937):240</Citation><ArticleIdList><ArticleId IdType="pubmed">12748632</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 May 24;361(9371):1767-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12781535</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Jul 26;362(9380):263-70</Citation><ArticleIdList><ArticleId IdType="pubmed">12892955</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2003 Oct;41(10):4521-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14532176</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Oct 25;362(9393):1353-8</Citation><ArticleIdList><ArticleId IdType="pubmed">14585636</ArticleId></ArticleIdList></Reference><Reference><Citation>Transfus Med. 2003 Oct;13(5):259-66</Citation><ArticleIdList><ArticleId IdType="pubmed">14617336</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2003 Nov 28;311(4):870-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14623261</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2004 Feb;10(2):294-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15030700</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1982 May;42(2):700-7</Citation><ArticleIdList><ArticleId IdType="pubmed">7086972</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 1984 Mar;19(3):388-93</Citation><ArticleIdList><ArticleId IdType="pubmed">6325490</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 1992 Sep;30(9):2338-45</Citation><ArticleIdList><ArticleId IdType="pubmed">1400999</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2002 Jun;40(6):2023-30</Citation><ArticleIdList><ArticleId IdType="pubmed">12037058</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2001 Sep;39(9):3110-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11526137</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D48 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002D48 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:15184444
|texte= Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15184444" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |