SrasV1, PubMed, Corpus, bibRecord, 002D13

[The expression and activity detection of a variant N protein of SARS-CoV].

Identifieur interne : 002D13 ( PubMed/Corpus ); précédent : 002D12; suivant : 002D14

[The expression and activity detection of a variant N protein of SARS-CoV].

Auteurs : Yang Qi ; Yu Zheng ; Cui-Li Shu ; Li Jiang ; Yan Hu ; Pan-Yong Mao ; Yun Cheng

Source :

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2004.

RBID : pubmed:15207085

English descriptors

KwdEn :
- Base Sequence, Escherichia coli (genetics), Escherichia coli (metabolism), Genetic Vectors, Glutathione Transferase (genetics), Humans, Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), SARS Virus (genetics), Sequence Deletion, Transfection.
MESH :
- chemical , biosynthesis : Nucleocapsid Proteins, Recombinant Fusion Proteins.
- chemical , genetics : Glutathione Transferase, Nucleocapsid Proteins, Recombinant Fusion Proteins.
- genetics : Escherichia coli, SARS Virus.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Fusion Proteins.
- metabolism : Escherichia coli.
- Base Sequence, Genetic Vectors, Humans, Sequence Deletion, Transfection.

Abstract

To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli.

PubMed: 15207085

Links to Exploration step

pubmed:15207085

Le document en format XML

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<author><name sortKey="Qi, Yang" sort="Qi, Yang" uniqKey="Qi Y" first="Yang" last="Qi">Yang Qi</name>
<affiliation><nlm:affiliation>Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. qiy2004@sohu.com</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zheng, Yu" sort="Zheng, Yu" uniqKey="Zheng Y" first="Yu" last="Zheng">Yu Zheng</name>
</author>
<author><name sortKey="Shu, Cui Li" sort="Shu, Cui Li" uniqKey="Shu C" first="Cui-Li" last="Shu">Cui-Li Shu</name>
</author>
<author><name sortKey="Jiang, Li" sort="Jiang, Li" uniqKey="Jiang L" first="Li" last="Jiang">Li Jiang</name>
</author>
<author><name sortKey="Hu, Yan" sort="Hu, Yan" uniqKey="Hu Y" first="Yan" last="Hu">Yan Hu</name>
</author>
<author><name sortKey="Mao, Pan Yong" sort="Mao, Pan Yong" uniqKey="Mao P" first="Pan-Yong" last="Mao">Pan-Yong Mao</name>
</author>
<author><name sortKey="Cheng, Yun" sort="Cheng, Yun" uniqKey="Cheng Y" first="Yun" last="Cheng">Yun Cheng</name>
</author>
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<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[The expression and activity detection of a variant N protein of SARS-CoV].</title>
<author><name sortKey="Qi, Yang" sort="Qi, Yang" uniqKey="Qi Y" first="Yang" last="Qi">Yang Qi</name>
<affiliation><nlm:affiliation>Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. qiy2004@sohu.com</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zheng, Yu" sort="Zheng, Yu" uniqKey="Zheng Y" first="Yu" last="Zheng">Yu Zheng</name>
</author>
<author><name sortKey="Shu, Cui Li" sort="Shu, Cui Li" uniqKey="Shu C" first="Cui-Li" last="Shu">Cui-Li Shu</name>
</author>
<author><name sortKey="Jiang, Li" sort="Jiang, Li" uniqKey="Jiang L" first="Li" last="Jiang">Li Jiang</name>
</author>
<author><name sortKey="Hu, Yan" sort="Hu, Yan" uniqKey="Hu Y" first="Yan" last="Hu">Yan Hu</name>
</author>
<author><name sortKey="Mao, Pan Yong" sort="Mao, Pan Yong" uniqKey="Mao P" first="Pan-Yong" last="Mao">Pan-Yong Mao</name>
</author>
<author><name sortKey="Cheng, Yun" sort="Cheng, Yun" uniqKey="Cheng Y" first="Yun" last="Cheng">Yun Cheng</name>
</author>
</analytic>
<series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title>
<idno type="ISSN">1007-8738</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Genetic Vectors</term>
<term>Glutathione Transferase (genetics)</term>
<term>Humans</term>
<term>Nucleocapsid Proteins (biosynthesis)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Recombinant Fusion Proteins (biosynthesis)</term>
<term>Recombinant Fusion Proteins (genetics)</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>SARS Virus (genetics)</term>
<term>Sequence Deletion</term>
<term>Transfection</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutathione Transferase</term>
<term>Nucleocapsid Proteins</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Genetic Vectors</term>
<term>Humans</term>
<term>Sequence Deletion</term>
<term>Transfection</term>
</keywords>
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</teiHeader>
<front><div type="abstract" xml:lang="en">To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15207085</PMID>
<DateCompleted><Year>2005</Year>
<Month>02</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN>
<JournalIssue CitedMedium="Print"><Volume>20</Volume>
<Issue>4</Issue>
<PubDate><Year>2004</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title>
<ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[The expression and activity detection of a variant N protein of SARS-CoV].</ArticleTitle>
<Pagination><MedlinePgn>422-4</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The N region gene of SARS-CoV was cloned by RT-PCR. The expression vector was constructed by DNA recombination. The recombinant plasmid was transformed into E.coli BL21(DE3). The expression of the fusion protein was detected by Western blot.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">(1) As compared with the sequences in GenBank, 20 bp were deleted in DNA sequence of the cloned N protein. (2) The fusion protein GST-N was soluble. Western blot analysis showed that the reaction of GST-N to anti-SARS-CoV sera was positive.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The pGEX-2T/N has been constructed and expressed in the form of fusion protein GST-N successfully, which lays the foundation for further study of SARS-CoV N protein.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qi</LastName>
<ForeName>Yang</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. qiy2004@sohu.com</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Yu</ForeName>
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<Author ValidYN="Y"><LastName>Shu</LastName>
<ForeName>Cui-li</ForeName>
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<Author ValidYN="Y"><LastName>Jiang</LastName>
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<Author ValidYN="Y"><LastName>Hu</LastName>
<ForeName>Yan</ForeName>
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</Author>
<Author ValidYN="Y"><LastName>Mao</LastName>
<ForeName>Pan-yong</ForeName>
<Initials>PY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Cheng</LastName>
<ForeName>Yun</ForeName>
<Initials>Y</Initials>
</Author>
</AuthorList>
<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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</Article>
<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA>
<NlmUniqueID>101139110</NlmUniqueID>
<ISSNLinking>1007-8738</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber>
<NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
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<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year>
<Month>6</Month>
<Day>23</Day>
<Hour>5</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="medline"><Year>2005</Year>
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<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15207085</ArticleId>
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</record>

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[The expression and activity detection of a variant N protein of SARS-CoV].

[The expression and activity detection of a variant N protein of SARS-CoV].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki