Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.
Identifieur interne : 002D02 ( PubMed/Corpus ); précédent : 002D01; suivant : 002D03Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.
Auteurs : Ji-Yong Zhou ; Li-Qin Cheng ; Xiao-Juan Zheng ; Jian-Xiang Wu ; Shao-Bin Shang ; Jin-Yong Wang ; Ji-Gang ChenSource :
- Journal of biotechnology [ 0168-1656 ] ; 2004.
English descriptors
- KwdEn :
- Animals, Chickens, Cloning, Molecular, Infectious bronchitis virus (genetics), Infectious bronchitis virus (metabolism), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Plants, Genetically Modified (metabolism), Protein Engineering (methods), Recombinant Proteins (biosynthesis), Recombinant Proteins (chemistry), Recombinant Proteins (immunology), Solanum tuberosum (genetics), Solanum tuberosum (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , chemistry : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- genetics : Infectious bronchitis virus, Membrane Glycoproteins, Solanum tuberosum, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- metabolism : Infectious bronchitis virus, Plants, Genetically Modified, Solanum tuberosum.
- methods : Protein Engineering.
- Animals, Chickens, Cloning, Molecular, Spike Glycoprotein, Coronavirus.
Abstract
To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine.
DOI: 10.1016/j.jbiotec.2004.03.012
PubMed: 15219399
Links to Exploration step
pubmed:15219399Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.</title><author><name sortKey="Zhou, Ji Yong" sort="Zhou, Ji Yong" uniqKey="Zhou J" first="Ji-Yong" last="Zhou">Ji-Yong Zhou</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. jyzhou@zju.edu.cn</nlm:affiliation></affiliation></author><author><name sortKey="Cheng, Li Qin" sort="Cheng, Li Qin" uniqKey="Cheng L" first="Li-Qin" last="Cheng">Li-Qin Cheng</name></author><author><name sortKey="Zheng, Xiao Juan" sort="Zheng, Xiao Juan" uniqKey="Zheng X" first="Xiao-Juan" last="Zheng">Xiao-Juan Zheng</name></author><author><name sortKey="Wu, Jian Xiang" sort="Wu, Jian Xiang" uniqKey="Wu J" first="Jian-Xiang" last="Wu">Jian-Xiang Wu</name></author><author><name sortKey="Shang, Shao Bin" sort="Shang, Shao Bin" uniqKey="Shang S" first="Shao-Bin" last="Shang">Shao-Bin Shang</name></author><author><name sortKey="Wang, Jin Yong" sort="Wang, Jin Yong" uniqKey="Wang J" first="Jin-Yong" last="Wang">Jin-Yong Wang</name></author><author><name sortKey="Chen, Ji Gang" sort="Chen, Ji Gang" uniqKey="Chen J" first="Ji-Gang" last="Chen">Ji-Gang Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15219399</idno><idno type="pmid">15219399</idno><idno type="doi">10.1016/j.jbiotec.2004.03.012</idno><idno type="wicri:Area/PubMed/Corpus">002D02</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002D02</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.</title><author><name sortKey="Zhou, Ji Yong" sort="Zhou, Ji Yong" uniqKey="Zhou J" first="Ji-Yong" last="Zhou">Ji-Yong Zhou</name><affiliation><nlm:affiliation>Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. jyzhou@zju.edu.cn</nlm:affiliation></affiliation></author><author><name sortKey="Cheng, Li Qin" sort="Cheng, Li Qin" uniqKey="Cheng L" first="Li-Qin" last="Cheng">Li-Qin Cheng</name></author><author><name sortKey="Zheng, Xiao Juan" sort="Zheng, Xiao Juan" uniqKey="Zheng X" first="Xiao-Juan" last="Zheng">Xiao-Juan Zheng</name></author><author><name sortKey="Wu, Jian Xiang" sort="Wu, Jian Xiang" uniqKey="Wu J" first="Jian-Xiang" last="Wu">Jian-Xiang Wu</name></author><author><name sortKey="Shang, Shao Bin" sort="Shang, Shao Bin" uniqKey="Shang S" first="Shao-Bin" last="Shang">Shao-Bin Shang</name></author><author><name sortKey="Wang, Jin Yong" sort="Wang, Jin Yong" uniqKey="Wang J" first="Jin-Yong" last="Wang">Jin-Yong Wang</name></author><author><name sortKey="Chen, Ji Gang" sort="Chen, Ji Gang" uniqKey="Chen J" first="Ji-Gang" last="Chen">Ji-Gang Chen</name></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="ISSN">0168-1656</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Cloning, Molecular</term><term>Infectious bronchitis virus (genetics)</term><term>Infectious bronchitis virus (metabolism)</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Plants, Genetically Modified (metabolism)</term><term>Protein Engineering (methods)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (immunology)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term><term>Membrane Glycoproteins</term><term>Solanum tuberosum</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Infectious bronchitis virus</term><term>Plants, Genetically Modified</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Engineering</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Cloning, Molecular</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15219399</PMID><DateCompleted><Year>2005</Year><Month>01</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0168-1656</ISSN><JournalIssue CitedMedium="Print"><Volume>111</Volume><Issue>2</Issue><PubDate><Year>2004</Year><Month>Jul</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of biotechnology</Title><ISOAbbreviation>J. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus.</ArticleTitle><Pagination><MedlinePgn>121-30</MedlinePgn></Pagination><Abstract><AbstractText>To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Ji-Yong</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China. jyzhou@zju.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Li-Qin</ForeName><Initials>LQ</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Xiao-Juan</ForeName><Initials>XJ</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jian-Xiang</ForeName><Initials>JX</Initials></Author><Author ValidYN="Y"><LastName>Shang</LastName><ForeName>Shao-Bin</ForeName><Initials>SB</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jin-Yong</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Ji-Gang</ForeName><Initials>JG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biotechnol</MedlineTA><NlmUniqueID>8411927</NlmUniqueID><ISSNLinking>0168-1656</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" 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