Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein.
Identifieur interne : 002C19 ( PubMed/Corpus ); précédent : 002C18; suivant : 002C20Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein.
Auteurs : Tsanan Giroglou ; Jindrich Cinatl ; Holger Rabenau ; Christian Drosten ; Harald Schwalbe ; Hans Wilhelm Doerr ; Dorothee Von LaerSource :
- Journal of virology [ 0022-538X ] ; 2004.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Cats, Cell Line, Tumor, Cells, Cultured, Dogs, Genetic Engineering, Genetic Vectors (genetics), Genetic Vectors (physiology), Humans, Immune Sera (immunology), Leukemia Virus, Murine (genetics), Leukemia Virus, Murine (immunology), Leukemia Virus, Murine (physiology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Neutralization Tests, Organ Specificity, SARS Virus (classification), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (physiology), Sequence Deletion, Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (transmission), Severe Acute Respiratory Syndrome (virology), Species Specificity, Spike Glycoprotein, Coronavirus, Swine (virology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Zoonoses (transmission), Zoonoses (virology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Immune Sera, Membrane Glycoproteins, Viral Envelope Proteins.
- classification : SARS Virus.
- genetics : Genetic Vectors, Leukemia Virus, Murine, SARS Virus.
- immunology : Leukemia Virus, Murine, SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- physiology : Genetic Vectors, Leukemia Virus, Murine, SARS Virus.
- transmission : Severe Acute Respiratory Syndrome, Zoonoses.
- virology : Severe Acute Respiratory Syndrome, Swine, Zoonoses.
- Animals, Cats, Cell Line, Tumor, Cells, Cultured, Dogs, Genetic Engineering, Humans, Neutralization Tests, Organ Specificity, Sequence Deletion, Species Specificity, Spike Glycoprotein, Coronavirus.
Abstract
The worldwide outbreak of severe acute respiratory syndrome (SARS) was shown to be associated with a novel coronavirus (CoV) now called SARS CoV. We report here the generation of SARS CoV S protein-pseudotyped murine leukemia virus (MLV) vector particles. The wild-type S protein pseudotyped MLV vectors, although at a low efficiency. Partial deletion of the cytoplasmic tail of S dramatically increased infectivity of pseudotypes, with titers only two- to threefold lower than those of pseudotypes generated in parallel with the vesicular stomatitis virus G protein. S-pseudotyped MLV particles were used to analyze viral tropism. MLV(SARS) pseudotypes and wild-type SARS CoV displayed similar cell types and tissue and host restrictions, indicating that the expression of a functional receptor is the major restraint in permissiveness to SARS CoV infection. Efficient gene transfer could be detected in Vero and CaCo2 cells, whereas the level of gene marking of 293T, HeLa, and HepG2 cells was only slightly above background levels. A cat cell line and a dog cell line were not susceptible. Interestingly, PK-15, a porcine kidney cell line, and primary porcine kidney cells were also highly permissive for SARS S pseudotypes and wild-type SARS CoV. This finding suggests that swine may be susceptible to SARS infection and may be a source for infection of humans. Taken together, these results indicate that MLV(SARS) pseudotypes are highly valuable for functional studies of viral tropism and entry and, in addition, can be a powerful tool for the development of therapeutic entry inhibitors without posing a biohazard to human beings.
DOI: 10.1128/JVI.78.17.9007-9015.2004
PubMed: 15308697
Links to Exploration step
pubmed:15308697Le document en format XML
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xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>Swine</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Cell Line, Tumor</term><term>Cells, Cultured</term><term>Dogs</term><term>Genetic Engineering</term><term>Humans</term><term>Neutralization Tests</term><term>Organ Specificity</term><term>Sequence Deletion</term><term>Species Specificity</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The worldwide outbreak of severe acute respiratory syndrome (SARS) was shown to be associated with a novel coronavirus (CoV) now called SARS CoV. We report here the generation of SARS CoV S protein-pseudotyped murine leukemia virus (MLV) vector particles. The wild-type S protein pseudotyped MLV vectors, although at a low efficiency. Partial deletion of the cytoplasmic tail of S dramatically increased infectivity of pseudotypes, with titers only two- to threefold lower than those of pseudotypes generated in parallel with the vesicular stomatitis virus G protein. S-pseudotyped MLV particles were used to analyze viral tropism. MLV(SARS) pseudotypes and wild-type SARS CoV displayed similar cell types and tissue and host restrictions, indicating that the expression of a functional receptor is the major restraint in permissiveness to SARS CoV infection. Efficient gene transfer could be detected in Vero and CaCo2 cells, whereas the level of gene marking of 293T, HeLa, and HepG2 cells was only slightly above background levels. A cat cell line and a dog cell line were not susceptible. Interestingly, PK-15, a porcine kidney cell line, and primary porcine kidney cells were also highly permissive for SARS S pseudotypes and wild-type SARS CoV. This finding suggests that swine may be susceptible to SARS infection and may be a source for infection of humans. Taken together, these results indicate that MLV(SARS) pseudotypes are highly valuable for functional studies of viral tropism and entry and, in addition, can be a powerful tool for the development of therapeutic entry inhibitors without posing a biohazard to human beings.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15308697</PMID><DateCompleted><Year>2004</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>78</Volume><Issue>17</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein.</ArticleTitle><Pagination><MedlinePgn>9007-15</MedlinePgn></Pagination><Abstract><AbstractText>The worldwide outbreak of severe acute respiratory syndrome (SARS) was shown to be associated with a novel coronavirus (CoV) now called SARS CoV. We report here the generation of SARS CoV S protein-pseudotyped murine leukemia virus (MLV) vector particles. The wild-type S protein pseudotyped MLV vectors, although at a low efficiency. Partial deletion of the cytoplasmic tail of S dramatically increased infectivity of pseudotypes, with titers only two- to threefold lower than those of pseudotypes generated in parallel with the vesicular stomatitis virus G protein. S-pseudotyped MLV particles were used to analyze viral tropism. MLV(SARS) pseudotypes and wild-type SARS CoV displayed similar cell types and tissue and host restrictions, indicating that the expression of a functional receptor is the major restraint in permissiveness to SARS CoV infection. Efficient gene transfer could be detected in Vero and CaCo2 cells, whereas the level of gene marking of 293T, HeLa, and HepG2 cells was only slightly above background levels. A cat cell line and a dog cell line were not susceptible. Interestingly, PK-15, a porcine kidney cell line, and primary porcine kidney cells were also highly permissive for SARS S pseudotypes and wild-type SARS CoV. This finding suggests that swine may be susceptible to SARS infection and may be a source for infection of humans. Taken together, these results indicate that MLV(SARS) pseudotypes are highly valuable for functional studies of viral tropism and entry and, in addition, can be a powerful tool for the development of therapeutic entry inhibitors without posing a biohazard to human beings.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Giroglou</LastName><ForeName>Tsanan</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Georg-Speyer-Haus, Institute for Biomedical Research, Frankfurt University Medical School, Frankfurt, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cinatl</LastName><ForeName>Jindrich</ForeName><Initials>J</Initials><Suffix>Jr</Suffix></Author><Author ValidYN="Y"><LastName>Rabenau</LastName><ForeName>Holger</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Drosten</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Schwalbe</LastName><ForeName>Harald</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Doerr</LastName><ForeName>Hans Wilhelm</ForeName><Initials>HW</Initials></Author><Author ValidYN="Y"><LastName>von Laer</LastName><ForeName>Dorothee</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C028391">G protein, vesicular stomatitis virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007106">Immune 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MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002415" MajorTopicYN="N">Cats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004285" MajorTopicYN="N">Dogs</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="Y">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007106" MajorTopicYN="N">Immune 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UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000635" MajorTopicYN="N">transmission</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, 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IdType="pubmed">9151877</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8640-5</Citation><ArticleIdList><ArticleId IdType="pubmed">9238030</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10803-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9380714</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Feb;72(2):1224-34</Citation><ArticleIdList><ArticleId IdType="pubmed">9445022</ArticleId></ArticleIdList></Reference><Reference><Citation>Hum Gene Ther. 1998 Jan 20;9(2):225-34</Citation><ArticleIdList><ArticleId IdType="pubmed">9472782</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Apr;72(4):3155-60</Citation><ArticleIdList><ArticleId IdType="pubmed">9525641</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Dec;72(12):9628-36</Citation><ArticleIdList><ArticleId IdType="pubmed">9811696</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1998 Dec;72(12):9873-80</Citation><ArticleIdList><ArticleId IdType="pubmed">9811723</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1999 Jul;73(7):6114-6</Citation><ArticleIdList><ArticleId IdType="pubmed">10364368</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1999 Sep;73(9):7607-18</Citation><ArticleIdList><ArticleId IdType="pubmed">10438851</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1999 Aug 15;261(1):70-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10441556</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2000 Feb;74(3):1393-406</Citation><ArticleIdList><ArticleId IdType="pubmed">10627550</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Ther. 2000 Nov;2(5):435-45</Citation><ArticleIdList><ArticleId IdType="pubmed">11082317</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2001 Jan 20;279(2):371-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11162792</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2002 Feb;76(3):1488-95</Citation><ArticleIdList><ArticleId IdType="pubmed">11773421</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Pharmacol Sci. 2002 Apr;23(4):177-83</Citation><ArticleIdList><ArticleId IdType="pubmed">11931993</ArticleId></ArticleIdList></Reference><Reference><Citation>Hum Gene Ther. 2002 May 1;13(7):803-13</Citation><ArticleIdList><ArticleId IdType="pubmed">11975847</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2002 Oct 29;12(21):R745-52</Citation><ArticleIdList><ArticleId IdType="pubmed">12419208</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2002 Dec 4;532(1-2):107-10</Citation><ArticleIdList><ArticleId IdType="pubmed">12459472</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2003 Mar;77(5):2866-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12584310</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2003 Mar 3;197(5):633-42</Citation><ArticleIdList><ArticleId IdType="pubmed">12615904</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1948-51</Citation><ArticleIdList><ArticleId IdType="pubmed">12748314</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 May 15;423(6937):240</Citation><ArticleIdList><ArticleId IdType="pubmed">12748632</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7271-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12761383</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Jul 26;362(9380):263-70</Citation><ArticleIdList><ArticleId IdType="pubmed">12892955</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Jul 26;362(9380):293-4</Citation><ArticleIdList><ArticleId IdType="pubmed">12892961</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 Oct 10;302(5643):276-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12958366</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Oct 30;425(6961):915</Citation><ArticleIdList><ArticleId IdType="pubmed">14586458</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4</Citation><ArticleIdList><ArticleId IdType="pubmed">388439</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1982 Jun;119(2):358-71</Citation><ArticleIdList><ArticleId IdType="pubmed">6281979</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1983 Dec;131(2):296-307</Citation><ArticleIdList><ArticleId IdType="pubmed">6318433</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1985 Dec;56(3):904-11</Citation><ArticleIdList><ArticleId IdType="pubmed">2999443</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1985 Dec;56(3):912-20</Citation><ArticleIdList><ArticleId IdType="pubmed">2999444</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1991 Mar;65(3):1202-7</Citation><ArticleIdList><ArticleId IdType="pubmed">1847450</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Cell Biol. 1994;43 Pt A:99-112</Citation><ArticleIdList><ArticleId IdType="pubmed">7823872</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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