[Construction of phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus].
Identifieur interne : 002B55 ( PubMed/Corpus ); précédent : 002B54; suivant : 002B56[Construction of phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus].
Auteurs : Jie Wu ; Wen-Ling Zheng ; Hong-Bin Zhang ; Jie Wang ; Yue-Hua Jiang ; Wen-Jie Huang ; Wen-Li MaSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2004.
English descriptors
- KwdEn :
- Antibodies, Viral (genetics), Bacteriophages (genetics), Escherichia coli (genetics), Genes, Immunoglobulin Heavy Chain, Genes, Immunoglobulin Light Chain, Humans, Immunoglobulin Fab Fragments (genetics), Immunoglobulin Heavy Chains (genetics), Immunoglobulin kappa-Chains (genetics), Immunoglobulin lambda-Chains (genetics), Peptide Library, Polymerase Chain Reaction, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , genetics : Antibodies, Viral, Immunoglobulin Fab Fragments, Immunoglobulin Heavy Chains, Immunoglobulin kappa-Chains, Immunoglobulin lambda-Chains.
- genetics : Bacteriophages, Escherichia coli.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- Genes, Immunoglobulin Heavy Chain, Genes, Immunoglobulin Light Chain, Humans, Peptide Library, Polymerase Chain Reaction.
Abstract
To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus.
PubMed: 15367356
Links to Exploration step
pubmed:15367356Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Construction of phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus].</title><author><name sortKey="Wu, Jie" sort="Wu, Jie" uniqKey="Wu J" first="Jie" last="Wu">Jie Wu</name><affiliation><nlm:affiliation>Institute of Cancer Molecular Biology,Guangzhou Command General Hospital, Guangzhou 510010, China. wujie55@hotmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Wen Ling" sort="Zheng, Wen Ling" uniqKey="Zheng W" first="Wen-Ling" last="Zheng">Wen-Ling Zheng</name></author><author><name sortKey="Zhang, Hong Bin" sort="Zhang, Hong Bin" uniqKey="Zhang H" first="Hong-Bin" last="Zhang">Hong-Bin Zhang</name></author><author><name sortKey="Wang, Jie" sort="Wang, Jie" uniqKey="Wang J" first="Jie" last="Wang">Jie Wang</name></author><author><name sortKey="Jiang, Yue Hua" sort="Jiang, Yue Hua" uniqKey="Jiang Y" first="Yue-Hua" last="Jiang">Yue-Hua Jiang</name></author><author><name sortKey="Huang, Wen Jie" sort="Huang, Wen Jie" uniqKey="Huang W" first="Wen-Jie" last="Huang">Wen-Jie Huang</name></author><author><name sortKey="Ma, Wen Li" sort="Ma, Wen Li" uniqKey="Ma W" first="Wen-Li" last="Ma">Wen-Li Ma</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15367356</idno><idno type="pmid">15367356</idno><idno type="wicri:Area/PubMed/Corpus">002B55</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Construction of phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus].</title><author><name sortKey="Wu, Jie" sort="Wu, Jie" uniqKey="Wu J" first="Jie" last="Wu">Jie Wu</name><affiliation><nlm:affiliation>Institute of Cancer Molecular Biology,Guangzhou Command General Hospital, Guangzhou 510010, China. wujie55@hotmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Wen Ling" sort="Zheng, Wen Ling" uniqKey="Zheng W" first="Wen-Ling" last="Zheng">Wen-Ling Zheng</name></author><author><name sortKey="Zhang, Hong Bin" sort="Zhang, Hong Bin" uniqKey="Zhang H" first="Hong-Bin" last="Zhang">Hong-Bin Zhang</name></author><author><name sortKey="Wang, Jie" sort="Wang, Jie" uniqKey="Wang J" first="Jie" last="Wang">Jie Wang</name></author><author><name sortKey="Jiang, Yue Hua" sort="Jiang, Yue Hua" uniqKey="Jiang Y" first="Yue-Hua" last="Jiang">Yue-Hua Jiang</name></author><author><name sortKey="Huang, Wen Jie" sort="Huang, Wen Jie" uniqKey="Huang W" first="Wen-Jie" last="Huang">Wen-Jie Huang</name></author><author><name sortKey="Ma, Wen Li" sort="Ma, Wen Li" uniqKey="Ma W" first="Wen-Li" last="Ma">Wen-Li Ma</name></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (genetics)</term><term>Bacteriophages (genetics)</term><term>Escherichia coli (genetics)</term><term>Genes, Immunoglobulin Heavy Chain</term><term>Genes, Immunoglobulin Light Chain</term><term>Humans</term><term>Immunoglobulin Fab Fragments (genetics)</term><term>Immunoglobulin Heavy Chains (genetics)</term><term>Immunoglobulin kappa-Chains (genetics)</term><term>Immunoglobulin lambda-Chains (genetics)</term><term>Peptide Library</term><term>Polymerase Chain Reaction</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fab Fragments</term><term>Immunoglobulin Heavy Chains</term><term>Immunoglobulin kappa-Chains</term><term>Immunoglobulin lambda-Chains</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteriophages</term><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Immunoglobulin Heavy Chain</term><term>Genes, Immunoglobulin Light Chain</term><term>Humans</term><term>Peptide Library</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15367356</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>04</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>5</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title><ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Construction of phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus].</ArticleTitle><Pagination><MedlinePgn>592-4</MedlinePgn></Pagination><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Total RNA was extracted from peripheral blood lymphocytes of a patient with SARS virus-specific IgG antibody and was transcribed reversely into cDNA. Using the cDNA as template, Fd and light chain of Fab genes were amplified by PCR with specific primer and were cloned into phagemid pComb3. Then the recombinant phagemid was electroporated into E.coli XL-1 Blue. The recombinant rate was detected by enzyme digestion analysis, and library repertoire was determined.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The antibody library of Fab fragment from a convalescent patient infected with SARS conronavirus was constructed. The recombinant rate of light chain and heavy chain of Fd gene were 91% and 75%, respectively. The library size was 7.23 x 10(7).</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Phage antibody library for human Fab fragment has been constructed successfully, which lays the foundation for further study.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Cancer Molecular Biology,Guangzhou Command General Hospital, Guangzhou 510010, China. wujie55@hotmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Wen-ling</ForeName><Initials>WL</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hong-bin</ForeName><Initials>HB</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Yue-hua</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Wen-jie</ForeName><Initials>WJ</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Wen-li</ForeName><Initials>WL</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA><NlmUniqueID>101139110</NlmUniqueID><ISSNLinking>1007-8738</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007140">Immunoglobulin Fab Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007143">Immunoglobulin Heavy Chains</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007145">Immunoglobulin kappa-Chains</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007146">Immunoglobulin lambda-Chains</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019151">Peptide Library</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001435" MajorTopicYN="N">Bacteriophages</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050438" MajorTopicYN="N">Genes, Immunoglobulin Heavy Chain</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050439" MajorTopicYN="N">Genes, Immunoglobulin Light Chain</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007140" MajorTopicYN="N">Immunoglobulin Fab Fragments</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007143" MajorTopicYN="N">Immunoglobulin Heavy Chains</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007145" MajorTopicYN="N">Immunoglobulin kappa-Chains</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007146" MajorTopicYN="N">Immunoglobulin lambda-Chains</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019151" MajorTopicYN="Y">Peptide Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>9</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>4</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>9</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15367356</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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