[A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person].
Identifieur interne : 002A51 ( PubMed/Corpus ); précédent : 002A50; suivant : 002A52[A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person].
Auteurs : Xing-Wang Jia ; Ya-Ping Tian ; Hong Shen ; Shu-Zhen Song ; Bin Yuan ; Xin-Xin DengSource :
- Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue [ 1003-0603 ] ; 2004.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Convalescence, Female, Health Personnel, Humans, Male, Middle Aged, Occupational Exposure, RNA, Viral (analysis), RNA, Viral (blood), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology), Young Adult.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , blood : RNA, Viral.
- blood : Severe Acute Respiratory Syndrome.
- diagnosis : Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Adult, Aged, Aged, 80 and over, Convalescence, Female, Health Personnel, Humans, Male, Middle Aged, Occupational Exposure, Young Adult.
Abstract
To observe the method of fluorescent-polymerase chain reaction (F-PCR) and gene-hip technique in detecting coronavirus in severe acute respiratory syndrome (SARS) and its value for clinical application.
PubMed: 15535903
Links to Exploration step
pubmed:15535903Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person].</title>
<author><name sortKey="Jia, Xing Wang" sort="Jia, Xing Wang" uniqKey="Jia X" first="Xing-Wang" last="Jia">Xing-Wang Jia</name>
<affiliation><nlm:affiliation>Department of Clinical Biochemistry, PLA General Hospital and Postgraduate Medical School, Beijing 100853, China. jiaxingw301@yahoo.com.cn</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Tian, Ya Ping" sort="Tian, Ya Ping" uniqKey="Tian Y" first="Ya-Ping" last="Tian">Ya-Ping Tian</name>
</author>
<author><name sortKey="Shen, Hong" sort="Shen, Hong" uniqKey="Shen H" first="Hong" last="Shen">Hong Shen</name>
</author>
<author><name sortKey="Song, Shu Zhen" sort="Song, Shu Zhen" uniqKey="Song S" first="Shu-Zhen" last="Song">Shu-Zhen Song</name>
</author>
<author><name sortKey="Yuan, Bin" sort="Yuan, Bin" uniqKey="Yuan B" first="Bin" last="Yuan">Bin Yuan</name>
</author>
<author><name sortKey="Deng, Xin Xin" sort="Deng, Xin Xin" uniqKey="Deng X" first="Xin-Xin" last="Deng">Xin-Xin Deng</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15535903</idno>
<idno type="pmid">15535903</idno>
<idno type="wicri:Area/PubMed/Corpus">002A51</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A51</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person].</title>
<author><name sortKey="Jia, Xing Wang" sort="Jia, Xing Wang" uniqKey="Jia X" first="Xing-Wang" last="Jia">Xing-Wang Jia</name>
<affiliation><nlm:affiliation>Department of Clinical Biochemistry, PLA General Hospital and Postgraduate Medical School, Beijing 100853, China. jiaxingw301@yahoo.com.cn</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Tian, Ya Ping" sort="Tian, Ya Ping" uniqKey="Tian Y" first="Ya-Ping" last="Tian">Ya-Ping Tian</name>
</author>
<author><name sortKey="Shen, Hong" sort="Shen, Hong" uniqKey="Shen H" first="Hong" last="Shen">Hong Shen</name>
</author>
<author><name sortKey="Song, Shu Zhen" sort="Song, Shu Zhen" uniqKey="Song S" first="Shu-Zhen" last="Song">Shu-Zhen Song</name>
</author>
<author><name sortKey="Yuan, Bin" sort="Yuan, Bin" uniqKey="Yuan B" first="Bin" last="Yuan">Bin Yuan</name>
</author>
<author><name sortKey="Deng, Xin Xin" sort="Deng, Xin Xin" uniqKey="Deng X" first="Xin-Xin" last="Deng">Xin-Xin Deng</name>
</author>
</analytic>
<series><title level="j">Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue</title>
<idno type="ISSN">1003-0603</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Convalescence</term>
<term>Female</term>
<term>Health Personnel</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Occupational Exposure</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (blood)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Severe Acute Respiratory Syndrome (blood)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Young Adult</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adolescent</term>
<term>Adult</term>
<term>Aged</term>
<term>Aged, 80 and over</term>
<term>Convalescence</term>
<term>Female</term>
<term>Health Personnel</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Occupational Exposure</term>
<term>Young Adult</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">To observe the method of fluorescent-polymerase chain reaction (F-PCR) and gene-hip technique in detecting coronavirus in severe acute respiratory syndrome (SARS) and its value for clinical application.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15535903</PMID>
<DateCompleted><Year>2009</Year>
<Month>09</Month>
<Day>08</Day>
</DateCompleted>
<DateRevised><Year>2004</Year>
<Month>11</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-0603</ISSN>
<JournalIssue CitedMedium="Print"><Volume>16</Volume>
<Issue>11</Issue>
<PubDate><Year>2004</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue</Title>
<ISOAbbreviation>Zhongguo Wei Zhong Bing Ji Jiu Yi Xue</ISOAbbreviation>
</Journal>
<ArticleTitle>[A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person].</ArticleTitle>
<Pagination><MedlinePgn>667-9</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To observe the method of fluorescent-polymerase chain reaction (F-PCR) and gene-hip technique in detecting coronavirus in severe acute respiratory syndrome (SARS) and its value for clinical application.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Serum of 60 SARS patients, 20 samples of serum and 20 samples of gargling fluid of medical staff of the "Fever Clinic", and one cDNA specimen obtained from one SARS suspect patient were examined with F-PCR diagnosis kit and gene-chip technique for SARS coronavirus.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Three methods, including DR Chip and two F-PCR kit from Zhongshan College Da'an gene stock company and Shanghai Fortune industrial joint-stock Co, Ltd, were used, and the results were all negative for all specimens, except one cDNA specimen, which was obtained from one SARS suspect patient, virus could be amplified by F-PCR.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Special RNA fragment of SARS virus has not been detected in gargling fluid and serum of SARS convalescents and medical staff.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jia</LastName>
<ForeName>Xing-wang</ForeName>
<Initials>XW</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Biochemistry, PLA General Hospital and Postgraduate Medical School, Beijing 100853, China. jiaxingw301@yahoo.com.cn</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Tian</LastName>
<ForeName>Ya-ping</ForeName>
<Initials>YP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Shen</LastName>
<ForeName>Hong</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Song</LastName>
<ForeName>Shu-zhen</ForeName>
<Initials>SZ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yuan</LastName>
<ForeName>Bin</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Deng</LastName>
<ForeName>Xin-xin</ForeName>
<Initials>XX</Initials>
</Author>
</AuthorList>
<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhongguo Wei Zhong Bing Ji Jiu Yi Xue</MedlineTA>
<NlmUniqueID>9887521</NlmUniqueID>
<ISSNLinking>1003-0603</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003289" MajorTopicYN="N">Convalescence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006282" MajorTopicYN="N">Health Personnel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016273" MajorTopicYN="N">Occupational Exposure</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year>
<Month>11</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2009</Year>
<Month>9</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2004</Year>
<Month>11</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15535903</ArticleId>
</ArticleIdList>
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