Molecular genetic methods in the diagnosis of lower respiratory tract infections.
Identifieur interne : 002961 ( PubMed/Corpus ); précédent : 002960; suivant : 002962Molecular genetic methods in the diagnosis of lower respiratory tract infections.
Auteurs : David R. MurdochSource :
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [ 0903-4641 ]
English descriptors
- KwdEn :
- Bacterial Infections (diagnosis), Bacterial Infections (microbiology), Bordetella pertussis (genetics), Bordetella pertussis (isolation & purification), Chlamydia (genetics), Chlamydia (isolation & purification), Haemophilus influenzae (genetics), Haemophilus influenzae (isolation & purification), Humans, Legionella (genetics), Legionella (isolation & purification), Molecular Biology (methods), Mycobacterium (genetics), Mycobacterium (isolation & purification), Mycoplasma pneumoniae (genetics), Mycoplasma pneumoniae (isolation & purification), Nucleic Acid Amplification Techniques, Pneumocystis carinii (genetics), Pneumocystis carinii (isolation & purification), Pneumonia, Pneumocystis (diagnosis), Pneumonia, Pneumocystis (microbiology), Polymerase Chain Reaction, Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (microbiology), SARS Virus (genetics), SARS Virus (isolation & purification), Streptococcus pneumoniae (genetics), Streptococcus pneumoniae (isolation & purification), Virus Diseases (diagnosis), Virus Diseases (virology).
- MESH :
- diagnosis : Bacterial Infections, Pneumonia, Pneumocystis, Respiratory Tract Infections, Virus Diseases.
- genetics : Bordetella pertussis, Chlamydia, Haemophilus influenzae, Legionella, Mycobacterium, Mycoplasma pneumoniae, Pneumocystis carinii, SARS Virus, Streptococcus pneumoniae.
- isolation & purification : Bordetella pertussis, Chlamydia, Haemophilus influenzae, Legionella, Mycobacterium, Mycoplasma pneumoniae, Pneumocystis carinii, SARS Virus, Streptococcus pneumoniae.
- methods : Molecular Biology.
- microbiology : Bacterial Infections, Pneumonia, Pneumocystis, Respiratory Tract Infections.
- virology : Virus Diseases.
- Humans, Nucleic Acid Amplification Techniques, Polymerase Chain Reaction.
Abstract
Molecular diagnostic techniques, such as PCR, have become useful tools for the rapid etiological diagnosis of lower respiratory tract infections. Nucleic acid amplification tests (NAATs) have been evaluated for detecting most respiratory pathogens, and commercial assays are available for some pathogens. However, standardized protocols are needed before these assays are introduced into routine diagnostic use. For pneumonia, NAATs offer advantages over conventional tests for the detection of Mycoplasma pneumoniae, Legionella spp. and Chlamydia pneumoniae. For pneumococcal pneumonia in adults, PCR adds little to existing diagnostic tests, and is unable to distinguish pneumococcal colonization from infection when testing respiratory samples. Although less sensitive than culture-based methods, several commercial molecular diagnostic assays have been developed for tuberculosis and are useful rapid tests for selected patients. PCR can now be considered the rapid diagnostic test of choice for pertussis and some respiratory virus infections. Further work is required to better characterize the role of molecular diagnostic tests for diagnosing lower respiratory tract infections, and to develop standard assays that can be readily adopted by routine diagnostic laboratories.
DOI: 10.1111/j.1600-0463.2004.apm11211-1202.x
PubMed: 15638835
Links to Exploration step
pubmed:15638835Le document en format XML
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Murdoch</name><affiliation><nlm:affiliation>Department of Pathology, Christchurch School of Medicine and Health Sciences, and Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand. david.murdoch@cdhb.govt.nz</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2004 Nov-Dec</MedlineDate></PubDate></date><idno type="RBID">pubmed:15638835</idno><idno type="pmid">15638835</idno><idno type="doi">10.1111/j.1600-0463.2004.apm11211-1202.x</idno><idno type="wicri:Area/PubMed/Corpus">002961</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002961</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular genetic methods in the diagnosis of lower respiratory tract infections.</title><author><name sortKey="Murdoch, David R" sort="Murdoch, David R" uniqKey="Murdoch D" first="David R" last="Murdoch">David R. Murdoch</name><affiliation><nlm:affiliation>Department of Pathology, Christchurch School of Medicine and Health Sciences, and Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand. david.murdoch@cdhb.govt.nz</nlm:affiliation></affiliation></author></analytic><series><title level="j">APMIS : acta pathologica, microbiologica, et immunologica Scandinavica</title><idno type="ISSN">0903-4641</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Infections (diagnosis)</term><term>Bacterial Infections (microbiology)</term><term>Bordetella pertussis (genetics)</term><term>Bordetella pertussis (isolation & purification)</term><term>Chlamydia (genetics)</term><term>Chlamydia (isolation & purification)</term><term>Haemophilus influenzae (genetics)</term><term>Haemophilus influenzae (isolation & purification)</term><term>Humans</term><term>Legionella (genetics)</term><term>Legionella (isolation & purification)</term><term>Molecular Biology (methods)</term><term>Mycobacterium (genetics)</term><term>Mycobacterium (isolation & purification)</term><term>Mycoplasma pneumoniae (genetics)</term><term>Mycoplasma pneumoniae (isolation & purification)</term><term>Nucleic Acid Amplification Techniques</term><term>Pneumocystis carinii (genetics)</term><term>Pneumocystis carinii (isolation & purification)</term><term>Pneumonia, Pneumocystis (diagnosis)</term><term>Pneumonia, Pneumocystis (microbiology)</term><term>Polymerase Chain Reaction</term><term>Respiratory Tract Infections (diagnosis)</term><term>Respiratory Tract Infections (microbiology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Streptococcus pneumoniae (genetics)</term><term>Streptococcus pneumoniae (isolation & purification)</term><term>Virus Diseases (diagnosis)</term><term>Virus Diseases (virology)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bacterial Infections</term><term>Pneumonia, Pneumocystis</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bordetella pertussis</term><term>Chlamydia</term><term>Haemophilus influenzae</term><term>Legionella</term><term>Mycobacterium</term><term>Mycoplasma pneumoniae</term><term>Pneumocystis carinii</term><term>SARS Virus</term><term>Streptococcus pneumoniae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bordetella pertussis</term><term>Chlamydia</term><term>Haemophilus influenzae</term><term>Legionella</term><term>Mycobacterium</term><term>Mycoplasma pneumoniae</term><term>Pneumocystis carinii</term><term>SARS Virus</term><term>Streptococcus pneumoniae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Biology</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bacterial Infections</term><term>Pneumonia, Pneumocystis</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Virus Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Nucleic Acid Amplification Techniques</term><term>Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Molecular diagnostic techniques, such as PCR, have become useful tools for the rapid etiological diagnosis of lower respiratory tract infections. Nucleic acid amplification tests (NAATs) have been evaluated for detecting most respiratory pathogens, and commercial assays are available for some pathogens. However, standardized protocols are needed before these assays are introduced into routine diagnostic use. For pneumonia, NAATs offer advantages over conventional tests for the detection of Mycoplasma pneumoniae, Legionella spp. and Chlamydia pneumoniae. For pneumococcal pneumonia in adults, PCR adds little to existing diagnostic tests, and is unable to distinguish pneumococcal colonization from infection when testing respiratory samples. Although less sensitive than culture-based methods, several commercial molecular diagnostic assays have been developed for tuberculosis and are useful rapid tests for selected patients. PCR can now be considered the rapid diagnostic test of choice for pertussis and some respiratory virus infections. Further work is required to better characterize the role of molecular diagnostic tests for diagnosing lower respiratory tract infections, and to develop standard assays that can be readily adopted by routine diagnostic laboratories.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15638835</PMID><DateCompleted><Year>2005</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0903-4641</ISSN><JournalIssue CitedMedium="Print"><Volume>112</Volume><Issue>11-12</Issue><PubDate><MedlineDate>2004 Nov-Dec</MedlineDate></PubDate></JournalIssue><Title>APMIS : acta pathologica, microbiologica, et immunologica Scandinavica</Title><ISOAbbreviation>APMIS</ISOAbbreviation></Journal><ArticleTitle>Molecular genetic methods in the diagnosis of lower respiratory tract infections.</ArticleTitle><Pagination><MedlinePgn>713-27</MedlinePgn></Pagination><Abstract><AbstractText>Molecular diagnostic techniques, such as PCR, have become useful tools for the rapid etiological diagnosis of lower respiratory tract infections. Nucleic acid amplification tests (NAATs) have been evaluated for detecting most respiratory pathogens, and commercial assays are available for some pathogens. However, standardized protocols are needed before these assays are introduced into routine diagnostic use. For pneumonia, NAATs offer advantages over conventional tests for the detection of Mycoplasma pneumoniae, Legionella spp. and Chlamydia pneumoniae. For pneumococcal pneumonia in adults, PCR adds little to existing diagnostic tests, and is unable to distinguish pneumococcal colonization from infection when testing respiratory samples. Although less sensitive than culture-based methods, several commercial molecular diagnostic assays have been developed for tuberculosis and are useful rapid tests for selected patients. PCR can now be considered the rapid diagnostic test of choice for pertussis and some respiratory virus infections. Further work is required to better characterize the role of molecular diagnostic tests for diagnosing lower respiratory tract infections, and to develop standard assays that can be readily adopted by routine diagnostic laboratories.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Murdoch</LastName><ForeName>David R</ForeName><Initials>DR</Initials><AffiliationInfo><Affiliation>Department of Pathology, Christchurch School of Medicine and Health Sciences, and Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand. david.murdoch@cdhb.govt.nz</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016454">Review</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>APMIS</MedlineTA><NlmUniqueID>8803400</NlmUniqueID><ISSNLinking>0903-4641</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001424" MajorTopicYN="N">Bacterial Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001886" MajorTopicYN="N">Bordetella pertussis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002689" MajorTopicYN="N">Chlamydia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006193" MajorTopicYN="N">Haemophilus influenzae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007875" MajorTopicYN="N">Legionella</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008967" MajorTopicYN="N">Molecular Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009161" MajorTopicYN="N">Mycobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009177" MajorTopicYN="N">Mycoplasma pneumoniae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="N">Nucleic Acid Amplification Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045363" MajorTopicYN="N">Pneumocystis carinii</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011020" MajorTopicYN="N">Pneumonia, Pneumocystis</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012141" MajorTopicYN="N">Respiratory Tract Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013296" MajorTopicYN="N">Streptococcus pneumoniae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014777" 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