[Development and preliminary application of monoclonal antibodies against N protein of SARS virus].
Identifieur interne : 002956 ( PubMed/Corpus ); précédent : 002955; suivant : 002957[Development and preliminary application of monoclonal antibodies against N protein of SARS virus].
Auteurs : Xiu-Hua Li ; You-Chun WangSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2004.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , immunology : Nucleocapsid Proteins.
- immunology : SARS Virus.
- Animals, Humans, Hybridomas, Mice, Mice, Inbred BALB C, Sensitivity and Specificity.
Abstract
To develop the monoclonal antibody against N protein of SARS virus and study its applicability.
PubMed: 15640861
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pubmed:15640861Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Development and preliminary application of monoclonal antibodies against N protein of SARS virus].</title><author><name sortKey="Li, Xiu Hua" sort="Li, Xiu Hua" uniqKey="Li X" first="Xiu-Hua" last="Li">Xiu-Hua Li</name><affiliation><nlm:affiliation>Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, You Chun" sort="Wang, You Chun" uniqKey="Wang Y" first="You-Chun" last="Wang">You-Chun Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15640861</idno><idno type="pmid">15640861</idno><idno type="wicri:Area/PubMed/Corpus">002956</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002956</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Development and preliminary application of monoclonal antibodies against N protein of SARS virus].</title><author><name sortKey="Li, Xiu Hua" sort="Li, Xiu Hua" uniqKey="Li X" first="Xiu-Hua" last="Li">Xiu-Hua Li</name><affiliation><nlm:affiliation>Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, You Chun" sort="Wang, You Chun" uniqKey="Wang Y" first="You-Chun" last="Wang">You-Chun Wang</name></author></analytic><series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title><idno type="ISSN">1003-9279</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Viral (biosynthesis)</term><term>Antigens, Viral (analysis)</term><term>Humans</term><term>Hybridomas</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Hybridomas</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To develop the monoclonal antibody against N protein of SARS virus and study its applicability.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15640861</PMID><DateCompleted><Year>2006</Year><Month>08</Month><Day>08</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN><JournalIssue CitedMedium="Print"><Volume>18</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title><ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Development and preliminary application of monoclonal antibodies against N protein of SARS virus].</ArticleTitle><Pagination><MedlinePgn>255-7</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To develop the monoclonal antibody against N protein of SARS virus and study its applicability.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">BALB/c mice were immunized with recombinant N protein. Spleen cells were collected and infused with SP2/0 cell. The infused cells were screened for anti-N protein antibody with ELISA. The positive cells were cloned and injected into abdominal cavity. The antibodies were purified from ascites. The affinities of those purified antibodies were analyzed with ELISA. The ELISA for detection of SARS virus antigen was developed by using antibody with the highest affinity. Its sensitivity and specificity were also evaluated primarily.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Eleven monoclonal cells secreting antibody have been developed. Three of the 11 purified monoclonal antibodies had very high affinity to N protein, while 4 purified McAbs showed very weak reaction to N protein, the affinities of remaining 4 McAbs were in between. The ELISA for detection of SARS virus antigen was developed with McAb 7. Its sensitivity was about 31 PFU/ml and had no cross reaction with other respiratory viruses.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The monoclonal antibody has good specificity and may be used to detect SARS virus antigen. However, its sensitivity is to be evaluated further with clinical samples from SARS patients, especially at acute phase.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiu-hua</ForeName><Initials>XH</Initials><AffiliationInfo><Affiliation>Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>You-chun</ForeName><Initials>YC</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA><NlmUniqueID>9602873</NlmUniqueID><ISSNLinking>1003-9279</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006825" MajorTopicYN="N">Hybridomas</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>8</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15640861</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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