Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination.
Identifieur interne : 002928 ( PubMed/Corpus ); précédent : 002927; suivant : 002929Expression of SARS-coronavirus nucleocapsid protein in Escherichia coli and Lactococcus lactis for serodiagnosis and mucosal vaccination.
Auteurs : Huadong Pei ; Jingfang Liu ; Yun Cheng ; Chaomin Sun ; Chen Wang ; Yueping Lu ; Jie Ding ; Jian Zhou ; Hua XiangSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2005.
English descriptors
- KwdEn :
- Administration, Intranasal, Administration, Oral, Animals, Antibodies, Viral (blood), Antibody Specificity, Escherichia coli (metabolism), Female, Glutathione Transferase (genetics), Glutathione Transferase (metabolism), Humans, Immunoglobulin G (blood), Lactococcus lactis (metabolism), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (metabolism), Recombinant Proteins (biosynthesis), Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), SARS Virus (immunology), Serologic Tests, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Vaccination, Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (immunology), Viral Vaccines (isolation & purification).
- MESH :
- chemical , administration & dosage : Vaccines, Synthetic, Viral Vaccines.
- chemical , biosynthesis : Recombinant Proteins.
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Glutathione Transferase, Nucleocapsid Proteins.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Proteins, Vaccines, Synthetic, Viral Vaccines.
- chemical , isolation & purification : Recombinant Proteins, Viral Vaccines.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- metabolism : Escherichia coli, Glutathione Transferase, Lactococcus lactis, Nucleocapsid Proteins.
- Administration, Intranasal, Administration, Oral, Animals, Antibody Specificity, Female, Humans, Mice, Mice, Inbred BALB C, Serologic Tests, Vaccination.
Abstract
The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.
DOI: 10.1007/s00253-004-1869-y
PubMed: 15660214
Links to Exploration step
pubmed:15660214Le document en format XML
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<term>Vaccination</term>
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<front><div type="abstract" xml:lang="en">The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</div>
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<Abstract><AbstractText>The nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) is an important antigen for the early diagnosis of SARS and the development of vaccines. It was expressed in Escherichia coli as a fusion with human glutathione S-transferase (hGST) and was confirmed by Western blotting analysis. This recombinant N protein (hGST-N) was purified and used to measure the SARS-CoV N-specific antibody in the sera of eight SARS patients by enzyme-linked immunosorbent assay. Specific antibody response to this purified recombinant N protein was 100% positive in the SARS patients' sera, while none of the control sera from 30 healthy people gave a positive reaction in the same assay. The SARS-CoV N protein was also expressed in Lactococcus lactis in the cytoplasm or secreted into the medium. The N-producing strain MG1363/pSECN and the purified hGST-N protein were respectively administered to mice, either orally or intranasally. Results indicated that orally delivered MG1363/pSECN induced significant N-specific IgG in the sera. In conclusion, our work provides a novel strategy to produce the SARS-CoV N protein for serodiagnosis and for L. lactis-based mucosal vaccines.</AbstractText>
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<ReferenceList><Reference><Citation>Lancet. 2003 May 24;361(9371):1779-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12781537</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Bacteriol. 1997 May;179(9):3068-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9139932</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Blood. 2003 Jul 1;102(1):223-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12649143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biomol Eng. 2003 Mar;20(3):107-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12684072</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Bacteriol. 1983 Apr;154(1):1-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6403500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Appl Environ Microbiol. 2003 Dec;69(12):7281-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14660377</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Biotechnol. 1997 Jul;15(7):653-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9219268</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Microbiol. 2004 May;42(5):2306-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15131220</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Immunol Lett. 2004 Apr 15;92(3):237-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15081618</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Bacteriol. 2001 Jul;183(14):4157-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11418555</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Virol. 2004 Aug;30(4):309-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15163419</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Clin Diagn Lab Immunol. 2004 Jan;11(1):227-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14715574</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Infect Immun. 2000 Jun;68(6):3251-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10816470</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biomol Eng. 2000 Jun;16(6):207-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10894115</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
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