Programmed ribosomal frameshifting in decoding the SARS-CoV genome.
Identifieur interne : 002917 ( PubMed/Corpus ); précédent : 002916; suivant : 002918Programmed ribosomal frameshifting in decoding the SARS-CoV genome.
Auteurs : Pavel V. Baranov ; Clark M. Henderson ; Christine B. Anderson ; Raymond F. Gesteland ; John F. Atkins ; Michael T. HowardSource :
- Virology [ 0042-6822 ] ; 2005.
English descriptors
- KwdEn :
- Base Sequence, Cell Line, Coronavirus (genetics), Frameshifting, Ribosomal (genetics), Genes, Reporter, Genome, Viral, Humans, Luciferases (genetics), Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Viral (chemistry), RNA, Viral (genetics), SARS Virus (genetics), Sequence Alignment, Spectrometry, Mass, Electrospray Ionization.
- MESH :
- chemical , chemistry : RNA, Viral.
- chemical , genetics : Luciferases, RNA, Viral.
- genetics : Coronavirus, Frameshifting, Ribosomal, SARS Virus.
- Base Sequence, Cell Line, Genes, Reporter, Genome, Viral, Humans, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, Sequence Alignment, Spectrometry, Mass, Electrospray Ionization.
Abstract
Programmed ribosomal frameshifting is an essential mechanism used for the expression of orf1b in coronaviruses. Comparative analysis of the frameshift region reveals a universal shift site U_UUA_AAC, followed by a predicted downstream RNA structure in the form of either a pseudoknot or kissing stem loops. Frameshifting in SARS-CoV has been characterized in cultured mammalian cells using a dual luciferase reporter system and mass spectrometry. Mutagenic analysis of the SARS-CoV shift site and mass spectrometry of an affinity tagged frameshift product confirmed tandem tRNA slippage on the sequence U_UUA_AAC. Analysis of the downstream pseudoknot stimulator of frameshifting in SARS-CoV shows that a proposed RNA secondary structure in loop II and two unpaired nucleotides at the stem I-stem II junction in SARS-CoV are important for frameshift stimulation. These results demonstrate key sequences required for efficient frameshifting, and the utility of mass spectrometry to study ribosomal frameshifting.
DOI: 10.1016/j.virol.2004.11.038
PubMed: 15680415
Links to Exploration step
pubmed:15680415Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Programmed ribosomal frameshifting in decoding the SARS-CoV genome.</title><author><name sortKey="Baranov, Pavel V" sort="Baranov, Pavel V" uniqKey="Baranov P" first="Pavel V" last="Baranov">Pavel V. Baranov</name><affiliation><nlm:affiliation>Department of Human Genetics, University of Utah, 15 N 2030 E, Room 7410, Salt Lake City, UT 84112-5330, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Henderson, Clark M" sort="Henderson, Clark M" uniqKey="Henderson C" first="Clark M" last="Henderson">Clark M. Henderson</name></author><author><name sortKey="Anderson, Christine B" sort="Anderson, Christine B" uniqKey="Anderson C" first="Christine B" last="Anderson">Christine B. Anderson</name></author><author><name sortKey="Gesteland, Raymond F" sort="Gesteland, Raymond F" uniqKey="Gesteland R" first="Raymond F" last="Gesteland">Raymond F. Gesteland</name></author><author><name sortKey="Atkins, John F" sort="Atkins, John F" uniqKey="Atkins J" first="John F" last="Atkins">John F. Atkins</name></author><author><name sortKey="Howard, Michael T" sort="Howard, Michael T" uniqKey="Howard M" first="Michael T" last="Howard">Michael T. Howard</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15680415</idno><idno type="pmid">15680415</idno><idno type="doi">10.1016/j.virol.2004.11.038</idno><idno type="wicri:Area/PubMed/Corpus">002917</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002917</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Programmed ribosomal frameshifting in decoding the SARS-CoV genome.</title><author><name sortKey="Baranov, Pavel V" sort="Baranov, Pavel V" uniqKey="Baranov P" first="Pavel V" last="Baranov">Pavel V. Baranov</name><affiliation><nlm:affiliation>Department of Human Genetics, University of Utah, 15 N 2030 E, Room 7410, Salt Lake City, UT 84112-5330, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Henderson, Clark M" sort="Henderson, Clark M" uniqKey="Henderson C" first="Clark M" last="Henderson">Clark M. Henderson</name></author><author><name sortKey="Anderson, Christine B" sort="Anderson, Christine B" uniqKey="Anderson C" first="Christine B" last="Anderson">Christine B. Anderson</name></author><author><name sortKey="Gesteland, Raymond F" sort="Gesteland, Raymond F" uniqKey="Gesteland R" first="Raymond F" last="Gesteland">Raymond F. Gesteland</name></author><author><name sortKey="Atkins, John F" sort="Atkins, John F" uniqKey="Atkins J" first="John F" last="Atkins">John F. Atkins</name></author><author><name sortKey="Howard, Michael T" sort="Howard, Michael T" uniqKey="Howard M" first="Michael T" last="Howard">Michael T. Howard</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Cell Line</term><term>Coronavirus (genetics)</term><term>Frameshifting, Ribosomal (genetics)</term><term>Genes, Reporter</term><term>Genome, Viral</term><term>Humans</term><term>Luciferases (genetics)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>RNA, Viral (chemistry)</term><term>RNA, Viral (genetics)</term><term>SARS Virus (genetics)</term><term>Sequence Alignment</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luciferases</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term><term>Frameshifting, Ribosomal</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cell Line</term><term>Genes, Reporter</term><term>Genome, Viral</term><term>Humans</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Sequence Alignment</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Programmed ribosomal frameshifting is an essential mechanism used for the expression of orf1b in coronaviruses. Comparative analysis of the frameshift region reveals a universal shift site U_UUA_AAC, followed by a predicted downstream RNA structure in the form of either a pseudoknot or kissing stem loops. Frameshifting in SARS-CoV has been characterized in cultured mammalian cells using a dual luciferase reporter system and mass spectrometry. Mutagenic analysis of the SARS-CoV shift site and mass spectrometry of an affinity tagged frameshift product confirmed tandem tRNA slippage on the sequence U_UUA_AAC. Analysis of the downstream pseudoknot stimulator of frameshifting in SARS-CoV shows that a proposed RNA secondary structure in loop II and two unpaired nucleotides at the stem I-stem II junction in SARS-CoV are important for frameshift stimulation. These results demonstrate key sequences required for efficient frameshifting, and the utility of mass spectrometry to study ribosomal frameshifting.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15680415</PMID><DateCompleted><Year>2005</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>332</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Feb</Month><Day>20</Day></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Programmed ribosomal frameshifting in decoding the SARS-CoV genome.</ArticleTitle><Pagination><MedlinePgn>498-510</MedlinePgn></Pagination><Abstract><AbstractText>Programmed ribosomal frameshifting is an essential mechanism used for the expression of orf1b in coronaviruses. Comparative analysis of the frameshift region reveals a universal shift site U_UUA_AAC, followed by a predicted downstream RNA structure in the form of either a pseudoknot or kissing stem loops. Frameshifting in SARS-CoV has been characterized in cultured mammalian cells using a dual luciferase reporter system and mass spectrometry. Mutagenic analysis of the SARS-CoV shift site and mass spectrometry of an affinity tagged frameshift product confirmed tandem tRNA slippage on the sequence U_UUA_AAC. Analysis of the downstream pseudoknot stimulator of frameshifting in SARS-CoV shows that a proposed RNA secondary structure in loop II and two unpaired nucleotides at the stem I-stem II junction in SARS-CoV are important for frameshift stimulation. These results demonstrate key sequences required for efficient frameshifting, and the utility of mass spectrometry to study ribosomal frameshifting.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Baranov</LastName><ForeName>Pavel V</ForeName><Initials>PV</Initials><AffiliationInfo><Affiliation>Department of Human Genetics, University of Utah, 15 N 2030 E, Room 7410, Salt Lake City, UT 84112-5330, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Henderson</LastName><ForeName>Clark M</ForeName><Initials>CM</Initials></Author><Author ValidYN="Y"><LastName>Anderson</LastName><ForeName>Christine B</ForeName><Initials>CB</Initials></Author><Author ValidYN="Y"><LastName>Gesteland</LastName><ForeName>Raymond F</ForeName><Initials>RF</Initials></Author><Author ValidYN="Y"><LastName>Atkins</LastName><ForeName>John F</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Howard</LastName><ForeName>Michael T</ForeName><Initials>MT</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 GM048152</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>GM48152</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virology</MedlineTA><NlmUniqueID>0110674</NlmUniqueID><ISSNLinking>0042-6822</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.12.-</RegistryNumber><NameOfSubstance UI="D008156">Luciferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018965" MajorTopicYN="N">Frameshifting, Ribosomal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008156" MajorTopicYN="N">Luciferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009690" MajorTopicYN="N">Nucleic Acid Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>08</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>11</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>2</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>4</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>2</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15680415</ArticleId><ArticleId IdType="pii">S0042-6822(04)00813-X</ArticleId><ArticleId IdType="doi">10.1016/j.virol.2004.11.038</ArticleId><ArticleId IdType="pmc">PMC7111862</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Virus Genes. 1990 Jul;4(2):121-36</Citation><ArticleIdList><ArticleId IdType="pubmed">2402881</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14234-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10588689</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1992 Sep 20;227(2):463-79</Citation><ArticleIdList><ArticleId IdType="pubmed">1404364</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 1998 Mar 15;26(6):1369-72</Citation><ArticleIdList><ArticleId IdType="pubmed">9490779</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Soc Trans. 2002 Nov;30(Pt 6):1126-31</Citation><ArticleIdList><ArticleId IdType="pubmed">12440988</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cold Spring Harb Symp Quant Biol. 2001;66:233-48</Citation><ArticleIdList><ArticleId IdType="pubmed">12762025</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2003 Sep;84(Pt 9):2305-2315</Citation><ArticleIdList><ArticleId IdType="pubmed">12917450</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1995 Apr 14;247(5):963-78</Citation><ArticleIdList><ArticleId IdType="pubmed">7723043</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):4899-903</Citation><ArticleIdList><ArticleId IdType="pubmed">11296253</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>RNA. 2002 Jan;8(1):16-28</Citation><ArticleIdList><ArticleId IdType="pubmed">11871658</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1996 May 31;259(1):135-47</Citation><ArticleIdList><ArticleId IdType="pubmed">8648641</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>RNA. 2004 Feb;10(2):221-30</Citation><ArticleIdList><ArticleId IdType="pubmed">14730021</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell. 2001 Jul 27;106(2):233-41</Citation><ArticleIdList><ArticleId IdType="pubmed">11511350</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2000 Apr 28;298(2):167-85</Citation><ArticleIdList><ArticleId IdType="pubmed">10764589</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1999 May 7;288(3):305-20</Citation><ArticleIdList><ArticleId IdType="pubmed">10329144</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1999 May 7;288(3):321-35</Citation><ArticleIdList><ArticleId IdType="pubmed">10329145</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell. 2004 Jan 30;13(2):157-68</Citation><ArticleIdList><ArticleId IdType="pubmed">14759362</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2004 Jun 4;339(3):495-504</Citation><ArticleIdList><ArticleId IdType="pubmed">15147837</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 2003 Jan 1;31(1):87-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12519954</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Struct Biol. 1999 Mar;6(3):285-92</Citation><ArticleIdList><ArticleId IdType="pubmed">10074948</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 1988 Jan 21;331(6153):280-3</Citation><ArticleIdList><ArticleId IdType="pubmed">2447506</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1996 Jul 26;260(4):479-83</Citation><ArticleIdList><ArticleId IdType="pubmed">8759314</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell. 1989 May 19;57(4):537-47</Citation><ArticleIdList><ArticleId IdType="pubmed">2720781</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1991 Aug 20;220(4):889-902</Citation><ArticleIdList><ArticleId IdType="pubmed">1880803</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 1997 May 15;25(10):1943-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9115361</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Trends Biochem Sci. 2002 Apr;27(4):178-83</Citation><ArticleIdList><ArticleId IdType="pubmed">11943544</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Gene. 2002 Mar 20;286(2):187-201</Citation><ArticleIdList><ArticleId IdType="pubmed">11943474</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 1999 May 21;288(5):911-40</Citation><ArticleIdList><ArticleId IdType="pubmed">10329189</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Adv Virus Res. 1992;41:193-239</Citation><ArticleIdList><ArticleId IdType="pubmed">1575083</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11676-81</Citation><ArticleIdList><ArticleId IdType="pubmed">12189204</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 2003 Jul 1;31(13):3406-15</Citation><ArticleIdList><ArticleId IdType="pubmed">12824337</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2001 Jun 22;309(5):1029-48</Citation><ArticleIdList><ArticleId IdType="pubmed">11399077</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>EMBO J. 1995 Feb 15;14(4):842-52</Citation><ArticleIdList><ArticleId IdType="pubmed">7882986</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>RNA. 1998 Apr;4(4):479-86</Citation><ArticleIdList><ArticleId IdType="pubmed">9630253</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Soc Trans. 2004 Dec;32(Pt 6):1081-3</Citation><ArticleIdList><ArticleId IdType="pubmed">15506971</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell. 2005 Jan 7;17(1):61-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15629717</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 1994 Nov 11;22(22):4673-80</Citation><ArticleIdList><ArticleId IdType="pubmed">7984417</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1991 Jun;65(6):2910-20</Citation><ArticleIdList><ArticleId IdType="pubmed">1851863</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 1995 May 11;23(9):1487-94</Citation><ArticleIdList><ArticleId IdType="pubmed">7784201</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Ann Neurol. 2000 Aug;48(2):164-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10939566</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nucleic Acids Res. 1993 Dec 25;21(25):5838-42</Citation><ArticleIdList><ArticleId IdType="pubmed">8290341</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002917 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002917 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:15680415
|texte= Programmed ribosomal frameshifting in decoding the SARS-CoV genome.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15680415" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |