Spike protein assembly into the coronavirion: exploring the limits of its sequence requirements.
Identifieur interne : 002833 ( PubMed/Corpus ); précédent : 002832; suivant : 002834Spike protein assembly into the coronavirion: exploring the limits of its sequence requirements.
Auteurs : Berend Jan Bosch ; Cornelis A M. De Haan ; Saskia L. Smits ; Peter J M. RottierSource :
- Virology [ 0042-6822 ] ; 2005.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cats, Cell Line, Coronavirus, Feline (genetics), Coronavirus, Feline (metabolism), Gene Expression Regulation, Viral, Humans, Membrane Fusion, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, Molecular Sequence Data, Murine hepatitis virus (genetics), Murine hepatitis virus (metabolism), RNA, Viral (genetics), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), Recombination, Genetic, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism), Virion (metabolism), Virus Assembly.
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Coronavirus, Feline, Membrane Glycoproteins, Murine hepatitis virus, RNA, Viral, Recombinant Fusion Proteins, Viral Envelope Proteins, Viral Matrix Proteins.
- metabolism : Coronavirus, Feline, Membrane Glycoproteins, Murine hepatitis virus, Recombinant Fusion Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Virion.
- Amino Acid Sequence, Animals, Base Sequence, Cats, Cell Line, Gene Expression Regulation, Viral, Humans, Membrane Fusion, Mice, Molecular Sequence Data, Recombination, Genetic, Spike Glycoprotein, Coronavirus, Virus Assembly.
Abstract
The coronavirus spike (S) protein, required for receptor binding and membrane fusion, is incorporated into the assembling virion by interactions with the viral membrane (M) protein. Earlier we showed that the ectodomain of the S protein is not involved in this process. Here we further defined the requirements of the S protein for virion incorporation. We show that the cytoplasmic domain, not the transmembrane domain, determines the association with the M protein and suffices to effect the incorporation into viral particles of chimeric spikes as well as of foreign viral glycoproteins. The essential sequence was mapped to the membrane-proximal region of the cytoplasmic domain, which is also known to be of critical importance for the fusion function of the S protein. Consistently, only short C-terminal truncations of the S protein were tolerated when introduced into the virus by targeted recombination. The important role of the about 38-residues cytoplasmic domain in the assembly of and membrane fusion by this approximately 1300 amino acids long protein is discussed.
DOI: 10.1016/j.virol.2005.02.001
PubMed: 15780881
Links to Exploration step
pubmed:15780881Le document en format XML
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<term>Coronavirus, Feline (genetics)</term>
<term>Coronavirus, Feline (metabolism)</term>
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<term>Humans</term>
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<term>Membrane Glycoproteins (metabolism)</term>
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<term>Murine hepatitis virus (metabolism)</term>
<term>RNA, Viral (genetics)</term>
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<term>Recombinant Fusion Proteins (metabolism)</term>
<term>Recombination, Genetic</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (genetics)</term>
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<term>Membrane Glycoproteins</term>
<term>Murine hepatitis virus</term>
<term>Recombinant Fusion Proteins</term>
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<term>Base Sequence</term>
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<term>Mice</term>
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<front><div type="abstract" xml:lang="en">The coronavirus spike (S) protein, required for receptor binding and membrane fusion, is incorporated into the assembling virion by interactions with the viral membrane (M) protein. Earlier we showed that the ectodomain of the S protein is not involved in this process. Here we further defined the requirements of the S protein for virion incorporation. We show that the cytoplasmic domain, not the transmembrane domain, determines the association with the M protein and suffices to effect the incorporation into viral particles of chimeric spikes as well as of foreign viral glycoproteins. The essential sequence was mapped to the membrane-proximal region of the cytoplasmic domain, which is also known to be of critical importance for the fusion function of the S protein. Consistently, only short C-terminal truncations of the S protein were tolerated when introduced into the virus by targeted recombination. The important role of the about 38-residues cytoplasmic domain in the assembly of and membrane fusion by this approximately 1300 amino acids long protein is discussed.</div>
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<Abstract><AbstractText>The coronavirus spike (S) protein, required for receptor binding and membrane fusion, is incorporated into the assembling virion by interactions with the viral membrane (M) protein. Earlier we showed that the ectodomain of the S protein is not involved in this process. Here we further defined the requirements of the S protein for virion incorporation. We show that the cytoplasmic domain, not the transmembrane domain, determines the association with the M protein and suffices to effect the incorporation into viral particles of chimeric spikes as well as of foreign viral glycoproteins. The essential sequence was mapped to the membrane-proximal region of the cytoplasmic domain, which is also known to be of critical importance for the fusion function of the S protein. Consistently, only short C-terminal truncations of the S protein were tolerated when introduced into the virus by targeted recombination. The important role of the about 38-residues cytoplasmic domain in the assembly of and membrane fusion by this approximately 1300 amino acids long protein is discussed.</AbstractText>
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</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 1990 Jan;64(1):339-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2403441</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2002 Apr 25;296(1):177-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12036329</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 Aug;74(15):6911-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10888630</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1985 May;54(2):374-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2985803</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1985 Dec;56(3):904-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2999443</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1997 Mar 17;16(6):1236-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9135140</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Gene. 1991 Dec 15;108(2):201-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1660838</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 Apr;76(8):3952-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11907235</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2003 Apr;77(8):4528-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12663759</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 May;76(9):4603-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11932425</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1996 Jul 1;221(1):87-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8661417</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1986 Mar;57(3):968-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3005639</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2001 Oct;75(19):9059-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11533169</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Cell Biol. 1994 Jan;124(1-2):55-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8294506</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Sep;73(9):7441-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10438834</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1991 Jan;180(1):395-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1984658</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1994 Sep 15;13(18):4204-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7925266</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1990 Jun;64(6):3051-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1692350</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1991 Sep;184(1):445-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1871979</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1994 Oct;68(10):6523-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8083990</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1988 Oct 15;263(29):14956-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2844793</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Cell Biol. 1995 Oct;131(2):339-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7593163</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2000 Oct 25;276(2):289-303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11040121</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1982 Jan 15;116(1):327-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6278712</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Arch Virol. 1987;95(1-2):123-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3036041</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2003 Nov;77(21):11312-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14557617</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2000 Apr 10;269(2):325-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10753711</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Oct;73(10):8703-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10482624</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1996 Apr 15;15(8):2020-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8617249</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 Feb;74(3):1566-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10627571</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1994 Jan;68(1):69-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8254778</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1992 Aug;66(8):4737-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1629953</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 1982 Sep 23;299(5881):371-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7110359</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1994 Nov 15;13(22):5504-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7957116</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 May;76(10):4987-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11967315</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1991 Dec;185(2):875-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1660205</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2002 Sep;76(18):9284-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12186912</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 Jun;74(11):4967-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10799570</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2000 Mar 30;269(1):212-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10725213</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1993 Jan;67(1):360-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8093220</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1992 Jul;189(1):167-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1604808</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1981 Nov;40(2):350-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6275093</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2000 May 10;270(2):464-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10793005</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1997 Dec;71(12):9278-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9371586</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 1995 Apr 14;270(15):8815-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7721788</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1990 Dec;9(12):3857-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2249653</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Cell Biol. 1988 May;106(5):1475-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2836431</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1998 Feb;72(2):1224-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9445022</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 Feb;74(3):1393-406</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10627550</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1992 Dec;66(12):7585-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1433532</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1990 Nov;64(11):5367-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2170676</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell. 1982 Oct;30(3):753-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6291783</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1996 Feb;70(2):873-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8551626</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1990 Sep;87(17):6743-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2204064</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1998 Dec;72(12):9747-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9811709</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Eur J Cell Biol. 1984 Mar;33(2):281-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6325194</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1998 Aug 10;17(5):1289-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9482726</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 May;74(9):4319-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10756047</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1996 Apr;70(4):2632-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8642698</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1987 Dec;161(2):479-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2825419</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1995 Dec 20;214(2):453-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8553547</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Mol Biol. 1981 Dec 25;153(4):993-1010</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7343686</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell. 1996 Mar 22;84(6):941-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8601317</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1984 Jul;3(7):1469-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6086325</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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