Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies.
Identifieur interne : 002765 ( PubMed/Corpus ); précédent : 002764; suivant : 002766Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies.
Auteurs : Kazuo Ohnishi ; Masahiro Sakaguchi ; Tomohiro Kaji ; Kiyoko Akagawa ; Tadayoshi Taniyama ; Masataka Kasai ; Yasuko Tsunetsugu-Yokota ; Masamichi Oshima ; Kiichi Yamamoto ; Naomi Takasuka ; Shu-Ichi Hashimoto ; Manabu Ato ; Hideki Fujii ; Yoshimasa Takahashi ; Shigeru Morikawa ; Koji Ishii ; Tetsutaro Sata ; Hirotaka Takagi ; Shigeyuki Itamura ; Takato Odagiri ; Tatsuo Miyamura ; Ichiro Kurane ; Masato Tashiro ; Takeshi Kurata ; Hiroshi Yoshikura ; Toshitada TakemoriSource :
- Japanese journal of infectious diseases [ 1344-6304 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical : Antibodies, Monoclonal.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- Animals, Chlorocebus aethiops, Female, Humans, Immunohistochemistry, Mice, Mice, Inbred BALB C, Sensitivity and Specificity, Vero Cells.
Abstract
In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.
PubMed: 15858286
Links to Exploration step
pubmed:15858286Le document en format XML
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<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
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<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
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<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
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<author><name sortKey="Miyamura, Tatsuo" sort="Miyamura, Tatsuo" uniqKey="Miyamura T" first="Tatsuo" last="Miyamura">Tatsuo Miyamura</name>
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<author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name>
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<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
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<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
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<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
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<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
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<author><name sortKey="Miyamura, Tatsuo" sort="Miyamura, Tatsuo" uniqKey="Miyamura T" first="Tatsuo" last="Miyamura">Tatsuo Miyamura</name>
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<author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name>
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<front><div type="abstract" xml:lang="en">In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.</div>
</front>
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<Abstract><AbstractText>In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.</AbstractText>
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