Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.
Identifieur interne : 002761 ( PubMed/Corpus ); précédent : 002760; suivant : 002762Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.
Auteurs : Pei-Lan Shao ; Po-Ren Hsueh ; Luan-Yin Chang ; Chun-Yi Lu ; Chuan-Liang Kao ; Yu-Ping Chiang ; Hsiang-Yi Huang ; Fu-Yuan Huang ; Chin-Yun Lee ; Lung-Ji Chang ; T-C Wu ; Li-Min HuangSource :
- Journal of biomedical science [ 1021-7770 ] ; 2005.
English descriptors
- KwdEn :
- Antigens, Viral (immunology), Enzyme-Linked Immunosorbent Assay (methods), Humans, Immunoglobulin G (analysis), SARS Virus (immunology), Sensitivity and Specificity, Serologic Tests (methods), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Statistics as Topic.
- MESH :
- chemical , analysis : Immunoglobulin G.
- chemical , immunology : Antigens, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- Humans, Sensitivity and Specificity, Statistics as Topic.
Abstract
Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.
DOI: 10.1007/s11373-004-8184-6
PubMed: 15864739
Links to Exploration step
pubmed:15864739Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.</title>
<author><name sortKey="Shao, Pei Lan" sort="Shao, Pei Lan" uniqKey="Shao P" first="Pei-Lan" last="Shao">Pei-Lan Shao</name>
<affiliation><nlm:affiliation>Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name>
</author>
<author><name sortKey="Chang, Luan Yin" sort="Chang, Luan Yin" uniqKey="Chang L" first="Luan-Yin" last="Chang">Luan-Yin Chang</name>
</author>
<author><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name>
</author>
<author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name>
</author>
<author><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name>
</author>
<author><name sortKey="Huang, Hsiang Yi" sort="Huang, Hsiang Yi" uniqKey="Huang H" first="Hsiang-Yi" last="Huang">Hsiang-Yi Huang</name>
</author>
<author><name sortKey="Huang, Fu Yuan" sort="Huang, Fu Yuan" uniqKey="Huang F" first="Fu-Yuan" last="Huang">Fu-Yuan Huang</name>
</author>
<author><name sortKey="Lee, Chin Yun" sort="Lee, Chin Yun" uniqKey="Lee C" first="Chin-Yun" last="Lee">Chin-Yun Lee</name>
</author>
<author><name sortKey="Chang, Lung Ji" sort="Chang, Lung Ji" uniqKey="Chang L" first="Lung-Ji" last="Chang">Lung-Ji Chang</name>
</author>
<author><name sortKey="Wu, T C" sort="Wu, T C" uniqKey="Wu T" first="T-C" last="Wu">T-C Wu</name>
</author>
<author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:15864739</idno>
<idno type="pmid">15864739</idno>
<idno type="doi">10.1007/s11373-004-8184-6</idno>
<idno type="wicri:Area/PubMed/Corpus">002761</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002761</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.</title>
<author><name sortKey="Shao, Pei Lan" sort="Shao, Pei Lan" uniqKey="Shao P" first="Pei-Lan" last="Shao">Pei-Lan Shao</name>
<affiliation><nlm:affiliation>Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name>
</author>
<author><name sortKey="Chang, Luan Yin" sort="Chang, Luan Yin" uniqKey="Chang L" first="Luan-Yin" last="Chang">Luan-Yin Chang</name>
</author>
<author><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name>
</author>
<author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name>
</author>
<author><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name>
</author>
<author><name sortKey="Huang, Hsiang Yi" sort="Huang, Hsiang Yi" uniqKey="Huang H" first="Hsiang-Yi" last="Huang">Hsiang-Yi Huang</name>
</author>
<author><name sortKey="Huang, Fu Yuan" sort="Huang, Fu Yuan" uniqKey="Huang F" first="Fu-Yuan" last="Huang">Fu-Yuan Huang</name>
</author>
<author><name sortKey="Lee, Chin Yun" sort="Lee, Chin Yun" uniqKey="Lee C" first="Chin-Yun" last="Lee">Chin-Yun Lee</name>
</author>
<author><name sortKey="Chang, Lung Ji" sort="Chang, Lung Ji" uniqKey="Chang L" first="Lung-Ji" last="Chang">Lung-Ji Chang</name>
</author>
<author><name sortKey="Wu, T C" sort="Wu, T C" uniqKey="Wu T" first="T-C" last="Wu">T-C Wu</name>
</author>
<author><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name>
</author>
</analytic>
<series><title level="j">Journal of biomedical science</title>
<idno type="ISSN">1021-7770</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Humans</term>
<term>Immunoglobulin G (analysis)</term>
<term>SARS Virus (immunology)</term>
<term>Sensitivity and Specificity</term>
<term>Serologic Tests (methods)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Statistics as Topic</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Serologic Tests</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Sensitivity and Specificity</term>
<term>Statistics as Topic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15864739</PMID>
<DateCompleted><Year>2005</Year>
<Month>10</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>25</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1021-7770</ISSN>
<JournalIssue CitedMedium="Print"><Volume>12</Volume>
<Issue>1</Issue>
<PubDate><Year>2005</Year>
</PubDate>
</JournalIssue>
<Title>Journal of biomedical science</Title>
<ISOAbbreviation>J. Biomed. Sci.</ISOAbbreviation>
</Journal>
<ArticleTitle>Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome.</ArticleTitle>
<Pagination><MedlinePgn>59-64</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shao</LastName>
<ForeName>Pei-Lan</ForeName>
<Initials>PL</Initials>
<AffiliationInfo><Affiliation>Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hsueh</LastName>
<ForeName>Po-Ren</ForeName>
<Initials>PR</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Luan-Yin</ForeName>
<Initials>LY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lu</LastName>
<ForeName>Chun-Yi</ForeName>
<Initials>CY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Kao</LastName>
<ForeName>Chuan-Liang</ForeName>
<Initials>CL</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chiang</LastName>
<ForeName>Yu-Ping</ForeName>
<Initials>YP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Hsiang-Yi</ForeName>
<Initials>HY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Fu-Yuan</ForeName>
<Initials>FY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>Chin-Yun</ForeName>
<Initials>CY</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Lung-Ji</ForeName>
<Initials>LJ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>T-C</ForeName>
<Initials>TC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Li-Min</ForeName>
<Initials>LM</Initials>
</Author>
<Author ValidYN="Y"><CollectiveName>SARS Research Group of National Taiwan University College of Medicine</CollectiveName>
</Author>
<Author ValidYN="Y"><CollectiveName>National Taiwan University Hospital</CollectiveName>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2005</Year>
<Month>02</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Biomed Sci</MedlineTA>
<NlmUniqueID>9421567</NlmUniqueID>
<ISSNLinking>1021-7770</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012698" MajorTopicYN="N">Serologic Tests</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013223" MajorTopicYN="N">Statistics as Topic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year>
<Month>06</Month>
<Day>17</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2004</Year>
<Month>08</Month>
<Day>25</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>5</Month>
<Day>3</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>10</Month>
<Day>28</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>5</Month>
<Day>3</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15864739</ArticleId>
<ArticleId IdType="doi">10.1007/s11373-004-8184-6</ArticleId>
<ArticleId IdType="pmc">PMC7088774</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Biomed Sci. 2003 Nov-Dec;10(6 Pt 2):664-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14631105</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1995-2005</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12671061</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Infect Dis. 2003 Apr 15;187(8):1187-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12695997</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol Methods. 2003 Mar;108(1):111-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12565161</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2003 Sep;9(9):1163-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14519257</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>JAMA. 2003 Jun 4;289(21):2801-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12734147</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1986-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12682352</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet. 2003 May 24;361(9371):1767-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12781535</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet. 2003 May 17;361(9370):1701-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12767737</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>MMWR Morb Mortal Wkly Rep. 2003 May 23;52(20):461-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12807078</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Clin Microbiol. 2002 May;40(5):1587-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11980926</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Med Virol. 2002 Dec;68(4):574-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12376966</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002761 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002761 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:15864739 |texte= Development of immunoglobulin G enzyme-linked immunosorbent assay for the serodiagnosis of severe acute respiratory syndrome. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15864739" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |