Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
Identifieur interne : 002754 ( PubMed/Corpus ); précédent : 002753; suivant : 002755Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
Auteurs : Maofeng Qiu ; Yuling Shi ; Zhaobiao Guo ; Zeliang Chen ; Rongqiao He ; Runsheng Chen ; Dongsheng Zhou ; Erhei Dai ; Xiaoyi Wang ; Bingyin Si ; Yajun Song ; Jingxiang Li ; Ling Yang ; Jin Wang ; Hongxia Wang ; Xin Pang ; Junhui Zhai ; Zongmin Du ; Ying Liu ; Yong Zhang ; Linhai Li ; Jian Wang ; Bing Sun ; Ruifu YangSource :
- Microbes and infection [ 1286-4579 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- chemical , immunology : Antibodies, Viral, Immunoglobulin G, Viral Proteins.
- immunology : SARS Virus.
- Animals, Humans, Neutralization Tests, Rabbits.
Abstract
A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.
DOI: 10.1016/j.micinf.2005.02.006
PubMed: 15878679
Links to Exploration step
pubmed:15878679Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.</title><author><name sortKey="Qiu, Maofeng" sort="Qiu, Maofeng" uniqKey="Qiu M" first="Maofeng" last="Qiu">Maofeng Qiu</name><affiliation><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name></author><author><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name></author><author><name sortKey="He, Rongqiao" sort="He, Rongqiao" uniqKey="He R" first="Rongqiao" last="He">Rongqiao He</name></author><author><name sortKey="Chen, Runsheng" sort="Chen, Runsheng" uniqKey="Chen R" first="Runsheng" last="Chen">Runsheng Chen</name></author><author><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name></author><author><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name></author><author><name sortKey="Song, Yajun" sort="Song, Yajun" uniqKey="Song Y" first="Yajun" last="Song">Yajun Song</name></author><author><name sortKey="Li, Jingxiang" sort="Li, Jingxiang" uniqKey="Li J" first="Jingxiang" last="Li">Jingxiang Li</name></author><author><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name></author><author><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name></author><author><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name></author><author><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name></author><author><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name></author><author><name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name></author><author><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name></author><author><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15878679</idno><idno type="pmid">15878679</idno><idno type="doi">10.1016/j.micinf.2005.02.006</idno><idno type="wicri:Area/PubMed/Corpus">002754</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002754</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.</title><author><name sortKey="Qiu, Maofeng" sort="Qiu, Maofeng" uniqKey="Qiu M" first="Maofeng" last="Qiu">Maofeng Qiu</name><affiliation><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name></author><author><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name></author><author><name sortKey="He, Rongqiao" sort="He, Rongqiao" uniqKey="He R" first="Rongqiao" last="He">Rongqiao He</name></author><author><name sortKey="Chen, Runsheng" sort="Chen, Runsheng" uniqKey="Chen R" first="Runsheng" last="Chen">Runsheng Chen</name></author><author><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name></author><author><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name></author><author><name sortKey="Song, Yajun" sort="Song, Yajun" uniqKey="Song Y" first="Yajun" last="Song">Yajun Song</name></author><author><name sortKey="Li, Jingxiang" sort="Li, Jingxiang" uniqKey="Li J" first="Jingxiang" last="Li">Jingxiang Li</name></author><author><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name></author><author><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name></author><author><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name></author><author><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name></author><author><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name></author><author><name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name></author><author><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name></author><author><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author></analytic><series><title level="j">Microbes and infection</title><idno type="ISSN">1286-4579</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (immunology)</term><term>Humans</term><term>Immunoglobulin G (immunology)</term><term>Neutralization Tests</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Viral Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Neutralization Tests</term><term>Rabbits</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15878679</PMID><DateCompleted><Year>2005</Year><Month>07</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1286-4579</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>5-6</Issue><PubDate><Year>2005</Year><Month>May</Month></PubDate></JournalIssue><Title>Microbes and infection</Title><ISOAbbreviation>Microbes Infect.</ISOAbbreviation></Journal><ArticleTitle>Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.</ArticleTitle><Pagination><MedlinePgn>882-9</MedlinePgn></Pagination><Abstract><AbstractText>A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Maofeng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Yuling</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Zhaobiao</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zeliang</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Rongqiao</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Runsheng</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Dongsheng</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Erhei</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiaoyi</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Si</LastName><ForeName>Bingyin</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Yajun</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jingxiang</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Ling</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jin</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hongxia</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Pang</LastName><ForeName>Xin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Zhai</LastName><ForeName>Junhui</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Zongmin</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ying</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yong</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Linhai</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jian</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Bing</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Ruifu</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>04</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Microbes Infect</MedlineTA><NlmUniqueID>100883508</NlmUniqueID><ISSNLinking>1286-4579</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009500" MajorTopicYN="Y">Neutralization Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>12</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2005</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>5</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>7</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>5</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15878679</ArticleId><ArticleId IdType="pii">S1286-4579(05)00086-9</ArticleId><ArticleId IdType="doi">10.1016/j.micinf.2005.02.006</ArticleId><ArticleId IdType="pmc">PMC7110836</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Infect Dis. 2004 Jul 15;190(2):379-86</Citation><ArticleIdList><ArticleId IdType="pubmed">15216476</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Curr Opin Microbiol. 2000 Jun;3(3):298-302</Citation><ArticleIdList><ArticleId IdType="pubmed">10851162</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Apr;78(7):3572-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15016880</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2004 Jul 1;190(1):91-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15195247</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2004 Oct;85(Pt 10):3109-3113</Citation><ArticleIdList><ArticleId IdType="pubmed">15448374</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 Jul 31;349(5):508-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12890855</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell Proteomics. 2003 May;2(5):346-56</Citation><ArticleIdList><ArticleId IdType="pubmed">12775768</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol Methods. 2001 Apr;250(1-2):81-91</Citation><ArticleIdList><ArticleId IdType="pubmed">11251223</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2000 Sep 8;289(5485):1760-3</Citation><ArticleIdList><ArticleId IdType="pubmed">10976071</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Med Virol. 2004 Jul;73(3):338-46</Citation><ArticleIdList><ArticleId IdType="pubmed">15170626</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9804-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15210961</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(13):7217-26</Citation><ArticleIdList><ArticleId IdType="pubmed">15194798</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Angew Chem Int Ed Engl. 2003 Feb 3;42(5):494-500</Citation><ArticleIdList><ArticleId IdType="pubmed">12569479</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2004 May 7;565(1-3):111-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15135062</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Chem. 2004 Jun;50(6):988-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15054081</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Trends Mol Med. 2002 Jun;8(6):250-3</Citation><ArticleIdList><ArticleId IdType="pubmed">12067604</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Diagn Lab Immunol. 2004 Mar;11(2):362-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15013989</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Microbiol. 2004 May;42(5):2306-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15131220</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Chin Sci Bull. 2003;48(10):941-948</Citation><ArticleIdList><ArticleId IdType="pubmed">32214698</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2004 Jul 30;341(1):271-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15312778</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6641-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15096611</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Diagn Lab Immunol. 2004 Mar;11(2):287-91</Citation><ArticleIdList><ArticleId IdType="pubmed">15013977</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Chem. 2002 Jan;48(1):121-30</Citation><ArticleIdList><ArticleId IdType="pubmed">11751547</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002754 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002754 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:15878679
|texte= Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:15878679" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |