Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.
Identifieur interne : 002739 ( PubMed/Corpus ); précédent : 002738; suivant : 002740Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.
Auteurs : Xiao Fei Yu ; Lin Hui Liang ; Ming She ; Xiao Long Liao ; Jie Gu ; Yan Han Li ; Zhong Chao HanSource :
- Immunology letters [ 0165-2478 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibody Specificity, Cell Line, Tumor, Chlorocebus aethiops, Hybridomas (cytology), Hybridomas (metabolism), Immunization, Male, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Plasmids, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Spleen (cytology), Spleen (immunology), Transfection, Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Vero Cells (virology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, DNA.
- chemical , biosynthesis : Antibodies, Monoclonal.
- chemical , genetics : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- cytology : Hybridomas, Spleen.
- immunology : SARS Virus, Spleen.
- metabolism : Hybridomas.
- virology : Vero Cells.
- Animals, Antibody Specificity, Cell Line, Tumor, Chlorocebus aethiops, Immunization, Male, Mice, Plasmids, Spike Glycoprotein, Coronavirus, Transfection.
Abstract
Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.
DOI: 10.1016/j.imlet.2005.03.015
PubMed: 15893826
Links to Exploration step
pubmed:15893826Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.</title><author><name sortKey="Yu, Xiao Fei" sort="Yu, Xiao Fei" uniqKey="Yu X" first="Xiao Fei" last="Yu">Xiao Fei Yu</name><affiliation><nlm:affiliation>State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Lin Hui" sort="Liang, Lin Hui" uniqKey="Liang L" first="Lin Hui" last="Liang">Lin Hui Liang</name></author><author><name sortKey="She, Ming" sort="She, Ming" uniqKey="She M" first="Ming" last="She">Ming She</name></author><author><name sortKey="Liao, Xiao Long" sort="Liao, Xiao Long" uniqKey="Liao X" first="Xiao Long" last="Liao">Xiao Long Liao</name></author><author><name sortKey="Gu, Jie" sort="Gu, Jie" uniqKey="Gu J" first="Jie" last="Gu">Jie Gu</name></author><author><name sortKey="Li, Yan Han" sort="Li, Yan Han" uniqKey="Li Y" first="Yan Han" last="Li">Yan Han Li</name></author><author><name sortKey="Han, Zhong Chao" sort="Han, Zhong Chao" uniqKey="Han Z" first="Zhong Chao" last="Han">Zhong Chao Han</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15893826</idno><idno type="pmid">15893826</idno><idno type="doi">10.1016/j.imlet.2005.03.015</idno><idno type="wicri:Area/PubMed/Corpus">002739</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002739</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.</title><author><name sortKey="Yu, Xiao Fei" sort="Yu, Xiao Fei" uniqKey="Yu X" first="Xiao Fei" last="Yu">Xiao Fei Yu</name><affiliation><nlm:affiliation>State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Lin Hui" sort="Liang, Lin Hui" uniqKey="Liang L" first="Lin Hui" last="Liang">Lin Hui Liang</name></author><author><name sortKey="She, Ming" sort="She, Ming" uniqKey="She M" first="Ming" last="She">Ming She</name></author><author><name sortKey="Liao, Xiao Long" sort="Liao, Xiao Long" uniqKey="Liao X" first="Xiao Long" last="Liao">Xiao Long Liao</name></author><author><name sortKey="Gu, Jie" sort="Gu, Jie" uniqKey="Gu J" first="Jie" last="Gu">Jie Gu</name></author><author><name sortKey="Li, Yan Han" sort="Li, Yan Han" uniqKey="Li Y" first="Yan Han" last="Li">Yan Han Li</name></author><author><name sortKey="Han, Zhong Chao" sort="Han, Zhong Chao" uniqKey="Han Z" first="Zhong Chao" last="Han">Zhong Chao Han</name></author></analytic><series><title level="j">Immunology letters</title><idno type="ISSN">0165-2478</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibody Specificity</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Hybridomas (cytology)</term><term>Hybridomas (metabolism)</term><term>Immunization</term><term>Male</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Plasmids</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Spleen (cytology)</term><term>Spleen (immunology)</term><term>Transfection</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Vero Cells (virology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Hybridomas</term><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hybridomas</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Cell Line, Tumor</term><term>Chlorocebus aethiops</term><term>Immunization</term><term>Male</term><term>Mice</term><term>Plasmids</term><term>Spike Glycoprotein, Coronavirus</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15893826</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0165-2478</ISSN><JournalIssue CitedMedium="Print"><Volume>100</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Sep</Month><Day>15</Day></PubDate></JournalIssue><Title>Immunology letters</Title><ISOAbbreviation>Immunol. Lett.</ISOAbbreviation></Journal><ArticleTitle>Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.</ArticleTitle><Pagination><MedlinePgn>177-81</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Xiao Fei</ForeName><Initials>XF</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Lin Hui</ForeName><Initials>LH</Initials></Author><Author ValidYN="Y"><LastName>She</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Xiao Long</ForeName><Initials>XL</Initials></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yan Han</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Zhong Chao</ForeName><Initials>ZC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Immunol Lett</MedlineTA><NlmUniqueID>7910006</NlmUniqueID><ISSNLinking>0165-2478</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="Y">Antibody Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045744" 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Commun. 2004 Feb 20;314(4):931-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14751221</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunol Lett. 2004 Sep;95(2):139-43</Citation><ArticleIdList><ArticleId IdType="pubmed">15388253</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Hybrid Hybridomics. 2002 Aug;21(4):287-93</Citation><ArticleIdList><ArticleId IdType="pubmed">12193282</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol Methods. 1984 May 11;70(1):39-43</Citation><ArticleIdList><ArticleId IdType="pubmed">6609209</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunol Lett. 2004 Apr 15;92(3):237-43</Citation><ArticleIdList><ArticleId IdType="pubmed">15081618</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol Methods. 2004 Sep 1;120(1):87-96</Citation><ArticleIdList><ArticleId IdType="pubmed">15234813</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2002 Jan;76(2):912-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11752180</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6641-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15096611</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2004 Sep;10(9):1653-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15498170</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Immunol Lett. 2001 Feb 1;76(1):25-30</Citation><ArticleIdList><ArticleId IdType="pubmed">11222909</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2536-41</Citation><ArticleIdList><ArticleId IdType="pubmed">14983044</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol Methods. 2000 Oct 20;244(1-2):1-7</Citation><ArticleIdList><ArticleId IdType="pubmed">11033013</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Oct;78(19):10628-35</Citation><ArticleIdList><ArticleId IdType="pubmed">15367630</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Sep;78(17):9007-15</Citation><ArticleIdList><ArticleId IdType="pubmed">15308697</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation><ArticleIdList><ArticleId IdType="pubmed">14670965</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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