Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS).
Identifieur interne : 002713 ( PubMed/Corpus ); précédent : 002712; suivant : 002714Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS).
Auteurs : Wai S. Chan ; Chun Wu ; Sammy C S. Chow ; To Cheung ; Ka-Fai To ; Wai-Keung Leung ; Paul K S. Chan ; Kam-Cheong Lee ; Ho-Keung Ng ; Deborah M Y. Au ; Anthony W I. LoSource :
- Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc [ 0893-3952 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Viral Structural Proteins.
- chemical , metabolism : Viral Structural Proteins.
- chemical : Antibodies, Viral.
- immunology : SARS Virus.
- virology : Enterocytes, Lung, Severe Acute Respiratory Syndrome.
- Animals, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence.
Abstract
Severe acute respiratory syndrome (SARS) is a newly emerging infectious disease that haunted the world from November 2002 to July 2003. Little is known about the biology and pathophysiology of the novel coronavirus that causes SARS. The tissue and cellular distributions of coronaviral hypothetical and structural proteins in SARS were investigated. Antibodies against the hypothetical (SARS 3a, 3b, 6, 7a and 9b) and structural proteins (envelope, membrane, nucleocapsid and spike) of the coronavirus were generated from predicted antigenic epitopes of each protein. The presence of these proteins were first verified in coronavirus-infected Vero E6 tissue culture model. Immunohistochemical studies on different human tissues, including a cohort of nine autopsies, two liver biopsies and intestinal biopsies of SARS patients, further confirmed the existence of coronaviral hypothetical and structural proteins in the cytoplasm of pneumocytes and small intestinal surface enterocytes in SARS patients. With this vast array of antibodies, no signal was observed in other cell types including those organs in which reverse transcriptase-polymerase chain reactions were reported to be positive. Structural proteins and the functionally undefined hypothetical proteins were expressed in coronavirus-infected cells with distinct expression pattern in different organs in SARS patients. These antipeptide antibodies can be useful for the diagnosis of SARS at the tissue level.
DOI: 10.1038/modpathol.3800439
PubMed: 15920543
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Chow</name></author><author><name sortKey="Cheung, To" sort="Cheung, To" uniqKey="Cheung T" first="To" last="Cheung">To Cheung</name></author><author><name sortKey="To, Ka Fai" sort="To, Ka Fai" uniqKey="To K" first="Ka-Fai" last="To">Ka-Fai To</name></author><author><name sortKey="Leung, Wai Keung" sort="Leung, Wai Keung" uniqKey="Leung W" first="Wai-Keung" last="Leung">Wai-Keung Leung</name></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></author><author><name sortKey="Lee, Kam Cheong" sort="Lee, Kam Cheong" uniqKey="Lee K" first="Kam-Cheong" last="Lee">Kam-Cheong Lee</name></author><author><name sortKey="Ng, Ho Keung" sort="Ng, Ho Keung" uniqKey="Ng H" first="Ho-Keung" last="Ng">Ho-Keung Ng</name></author><author><name sortKey="Au, Deborah M Y" sort="Au, Deborah M Y" uniqKey="Au D" first="Deborah M Y" last="Au">Deborah M Y. Au</name></author><author><name sortKey="Lo, Anthony W I" sort="Lo, Anthony W I" uniqKey="Lo A" first="Anthony W I" last="Lo">Anthony W I. Lo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15920543</idno><idno type="pmid">15920543</idno><idno type="doi">10.1038/modpathol.3800439</idno><idno type="wicri:Area/PubMed/Corpus">002713</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002713</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS).</title><author><name sortKey="Chan, Wai S" sort="Chan, Wai S" uniqKey="Chan W" first="Wai S" last="Chan">Wai S. Chan</name><affiliation><nlm:affiliation>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Chun" sort="Wu, Chun" uniqKey="Wu C" first="Chun" last="Wu">Chun Wu</name></author><author><name sortKey="Chow, Sammy C S" sort="Chow, Sammy C S" uniqKey="Chow S" first="Sammy C S" last="Chow">Sammy C S. Chow</name></author><author><name sortKey="Cheung, To" sort="Cheung, To" uniqKey="Cheung T" first="To" last="Cheung">To Cheung</name></author><author><name sortKey="To, Ka Fai" sort="To, Ka Fai" uniqKey="To K" first="Ka-Fai" last="To">Ka-Fai To</name></author><author><name sortKey="Leung, Wai Keung" sort="Leung, Wai Keung" uniqKey="Leung W" first="Wai-Keung" last="Leung">Wai-Keung Leung</name></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></author><author><name sortKey="Lee, Kam Cheong" sort="Lee, Kam Cheong" uniqKey="Lee K" first="Kam-Cheong" last="Lee">Kam-Cheong Lee</name></author><author><name sortKey="Ng, Ho Keung" sort="Ng, Ho Keung" uniqKey="Ng H" first="Ho-Keung" last="Ng">Ho-Keung Ng</name></author><author><name sortKey="Au, Deborah M Y" sort="Au, Deborah M Y" uniqKey="Au D" first="Deborah M Y" last="Au">Deborah M Y. Au</name></author><author><name sortKey="Lo, Anthony W I" sort="Lo, Anthony W I" uniqKey="Lo A" first="Anthony W I" last="Lo">Anthony W I. Lo</name></author></analytic><series><title level="j">Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc</title><idno type="ISSN">0893-3952</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral</term><term>Enterocytes (virology)</term><term>Humans</term><term>Immunohistochemistry</term><term>In Situ Hybridization, Fluorescence</term><term>Lung (virology)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Viral Structural Proteins (immunology)</term><term>Viral Structural Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Enterocytes</term><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Immunohistochemistry</term><term>In Situ Hybridization, Fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a newly emerging infectious disease that haunted the world from November 2002 to July 2003. Little is known about the biology and pathophysiology of the novel coronavirus that causes SARS. The tissue and cellular distributions of coronaviral hypothetical and structural proteins in SARS were investigated. Antibodies against the hypothetical (SARS 3a, 3b, 6, 7a and 9b) and structural proteins (envelope, membrane, nucleocapsid and spike) of the coronavirus were generated from predicted antigenic epitopes of each protein. The presence of these proteins were first verified in coronavirus-infected Vero E6 tissue culture model. Immunohistochemical studies on different human tissues, including a cohort of nine autopsies, two liver biopsies and intestinal biopsies of SARS patients, further confirmed the existence of coronaviral hypothetical and structural proteins in the cytoplasm of pneumocytes and small intestinal surface enterocytes in SARS patients. With this vast array of antibodies, no signal was observed in other cell types including those organs in which reverse transcriptase-polymerase chain reactions were reported to be positive. Structural proteins and the functionally undefined hypothetical proteins were expressed in coronavirus-infected cells with distinct expression pattern in different organs in SARS patients. These antipeptide antibodies can be useful for the diagnosis of SARS at the tissue level.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15920543</PMID><DateCompleted><Year>2006</Year><Month>01</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0893-3952</ISSN><JournalIssue CitedMedium="Print"><Volume>18</Volume><Issue>11</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc</Title><ISOAbbreviation>Mod. Pathol.</ISOAbbreviation></Journal><ArticleTitle>Coronaviral hypothetical and structural proteins were found in the intestinal surface enterocytes and pneumocytes of severe acute respiratory syndrome (SARS).</ArticleTitle><Pagination><MedlinePgn>1432-9</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is a newly emerging infectious disease that haunted the world from November 2002 to July 2003. Little is known about the biology and pathophysiology of the novel coronavirus that causes SARS. The tissue and cellular distributions of coronaviral hypothetical and structural proteins in SARS were investigated. Antibodies against the hypothetical (SARS 3a, 3b, 6, 7a and 9b) and structural proteins (envelope, membrane, nucleocapsid and spike) of the coronavirus were generated from predicted antigenic epitopes of each protein. The presence of these proteins were first verified in coronavirus-infected Vero E6 tissue culture model. Immunohistochemical studies on different human tissues, including a cohort of nine autopsies, two liver biopsies and intestinal biopsies of SARS patients, further confirmed the existence of coronaviral hypothetical and structural proteins in the cytoplasm of pneumocytes and small intestinal surface enterocytes in SARS patients. With this vast array of antibodies, no signal was observed in other cell types including those organs in which reverse transcriptase-polymerase chain reactions were reported to be positive. Structural proteins and the functionally undefined hypothetical proteins were expressed in coronavirus-infected cells with distinct expression pattern in different organs in SARS patients. These antipeptide antibodies can be useful for the diagnosis of SARS at the tissue level.</AbstractText><CopyrightInformation>Modern Pathology (2005) 18, 1432-1439. doi:10.1038/modpathol.3800439; published online 13 May 2005.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Wai S</ForeName><Initials>WS</Initials><AffiliationInfo><Affiliation>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Chun</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Chow</LastName><ForeName>Sammy C S</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Cheung</LastName><ForeName>To</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>To</LastName><ForeName>Ka-Fai</ForeName><Initials>KF</Initials></Author><Author ValidYN="Y"><LastName>Leung</LastName><ForeName>Wai-Keung</ForeName><Initials>WK</Initials></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Paul K S</ForeName><Initials>PK</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Kam-Cheong</ForeName><Initials>KC</Initials></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>Ho-Keung</ForeName><Initials>HK</Initials></Author><Author ValidYN="Y"><LastName>Au</LastName><ForeName>Deborah M Y</ForeName><Initials>DM</Initials></Author><Author ValidYN="Y"><LastName>Lo</LastName><ForeName>Anthony W I</ForeName><Initials>AW</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mod 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MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015678" MajorTopicYN="N">Viral Structural Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" 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