[An immunofluorescence assay for the detection of SARS associated coronavirus antibody based on recombinant nucleocapsid antigen and its application].
Identifieur interne : 002690 ( PubMed/Corpus ); précédent : 002689; suivant : 002691[An immunofluorescence assay for the detection of SARS associated coronavirus antibody based on recombinant nucleocapsid antigen and its application].
Auteurs : Han-Ping Zhu ; Zhi-Yong Zhu ; Guan-Mu Dong ; Ping-Ping Yao ; Qi An ; Jing-Qing Weng ; Yi-Yu Lu ; Fang Xu ; Qun-Ying Lu ; Ju-Ying Yan ; Qiong Ge ; Li-Ming Gong ; Wen Shi ; Min-Hong Li ; Zhi-Ya ZhaoSource :
- Zhonghua yi xue za zhi [ 0376-2491 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Nucleocapsid Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Adult, Female, Fluorescent Antibody Technique, Indirect, Humans, Male.
Abstract
To establish a new technique for SARS-CoV antibody test to detect infection of severer acute respiratory syndrome (SARS).
PubMed: 15949361
Links to Exploration step
pubmed:15949361Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[An immunofluorescence assay for the detection of SARS associated coronavirus antibody based on recombinant nucleocapsid antigen and its application].</title><author><name sortKey="Zhu, Han Ping" sort="Zhu, Han Ping" uniqKey="Zhu H" first="Han-Ping" last="Zhu">Han-Ping Zhu</name><affiliation><nlm:affiliation>Institute for Virus Disease Research, Center for Disease Prevention and Control of Zhejiang Province, Hangzhou 310009, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Zhi Yong" sort="Zhu, Zhi Yong" uniqKey="Zhu Z" first="Zhi-Yong" last="Zhu">Zhi-Yong Zhu</name></author><author><name sortKey="Dong, Guan Mu" sort="Dong, Guan Mu" uniqKey="Dong G" first="Guan-Mu" last="Dong">Guan-Mu Dong</name></author><author><name sortKey="Yao, Ping Ping" sort="Yao, Ping Ping" uniqKey="Yao P" first="Ping-Ping" last="Yao">Ping-Ping Yao</name></author><author><name sortKey="An, Qi" sort="An, Qi" uniqKey="An Q" first="Qi" last="An">Qi An</name></author><author><name sortKey="Weng, Jing Qing" sort="Weng, Jing Qing" uniqKey="Weng J" first="Jing-Qing" last="Weng">Jing-Qing Weng</name></author><author><name sortKey="Lu, Yi Yu" sort="Lu, Yi Yu" uniqKey="Lu Y" first="Yi-Yu" last="Lu">Yi-Yu Lu</name></author><author><name sortKey="Xu, Fang" sort="Xu, Fang" uniqKey="Xu F" first="Fang" last="Xu">Fang Xu</name></author><author><name sortKey="Lu, Qun Ying" sort="Lu, Qun Ying" uniqKey="Lu Q" first="Qun-Ying" last="Lu">Qun-Ying Lu</name></author><author><name sortKey="Yan, Ju Ying" sort="Yan, Ju Ying" uniqKey="Yan J" first="Ju-Ying" last="Yan">Ju-Ying Yan</name></author><author><name sortKey="Ge, Qiong" sort="Ge, Qiong" uniqKey="Ge Q" first="Qiong" last="Ge">Qiong Ge</name></author><author><name sortKey="Gong, Li Ming" sort="Gong, Li Ming" uniqKey="Gong L" first="Li-Ming" last="Gong">Li-Ming Gong</name></author><author><name sortKey="Shi, Wen" sort="Shi, Wen" uniqKey="Shi W" first="Wen" last="Shi">Wen Shi</name></author><author><name sortKey="Li, Min Hong" sort="Li, Min Hong" uniqKey="Li M" first="Min-Hong" last="Li">Min-Hong Li</name></author><author><name sortKey="Zhao, Zhi Ya" sort="Zhao, Zhi Ya" uniqKey="Zhao Z" first="Zhi-Ya" last="Zhao">Zhi-Ya Zhao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15949361</idno><idno type="pmid">15949361</idno><idno type="wicri:Area/PubMed/Corpus">002690</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002690</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[An immunofluorescence assay for the detection of SARS associated coronavirus antibody based on recombinant nucleocapsid antigen and its application].</title><author><name sortKey="Zhu, Han Ping" sort="Zhu, Han Ping" uniqKey="Zhu H" first="Han-Ping" last="Zhu">Han-Ping Zhu</name><affiliation><nlm:affiliation>Institute for Virus Disease Research, Center for Disease Prevention and Control of Zhejiang Province, Hangzhou 310009, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Zhi Yong" sort="Zhu, Zhi Yong" uniqKey="Zhu Z" first="Zhi-Yong" last="Zhu">Zhi-Yong Zhu</name></author><author><name sortKey="Dong, Guan Mu" sort="Dong, Guan Mu" uniqKey="Dong G" first="Guan-Mu" last="Dong">Guan-Mu Dong</name></author><author><name sortKey="Yao, Ping Ping" sort="Yao, Ping Ping" uniqKey="Yao P" first="Ping-Ping" last="Yao">Ping-Ping Yao</name></author><author><name sortKey="An, Qi" sort="An, Qi" uniqKey="An Q" first="Qi" last="An">Qi An</name></author><author><name sortKey="Weng, Jing Qing" sort="Weng, Jing Qing" uniqKey="Weng J" first="Jing-Qing" last="Weng">Jing-Qing Weng</name></author><author><name sortKey="Lu, Yi Yu" sort="Lu, Yi Yu" uniqKey="Lu Y" first="Yi-Yu" last="Lu">Yi-Yu Lu</name></author><author><name sortKey="Xu, Fang" sort="Xu, Fang" uniqKey="Xu F" first="Fang" last="Xu">Fang Xu</name></author><author><name sortKey="Lu, Qun Ying" sort="Lu, Qun Ying" uniqKey="Lu Q" first="Qun-Ying" last="Lu">Qun-Ying Lu</name></author><author><name sortKey="Yan, Ju Ying" sort="Yan, Ju Ying" uniqKey="Yan J" first="Ju-Ying" last="Yan">Ju-Ying Yan</name></author><author><name sortKey="Ge, Qiong" sort="Ge, Qiong" uniqKey="Ge Q" first="Qiong" last="Ge">Qiong Ge</name></author><author><name sortKey="Gong, Li Ming" sort="Gong, Li Ming" uniqKey="Gong L" first="Li-Ming" last="Gong">Li-Ming Gong</name></author><author><name sortKey="Shi, Wen" sort="Shi, Wen" uniqKey="Shi W" first="Wen" last="Shi">Wen Shi</name></author><author><name sortKey="Li, Min Hong" sort="Li, Min Hong" uniqKey="Li M" first="Min-Hong" last="Li">Min-Hong Li</name></author><author><name sortKey="Zhao, Zhi Ya" sort="Zhao, Zhi Ya" uniqKey="Zhao Z" first="Zhi-Ya" last="Zhao">Zhi-Ya Zhao</name></author></analytic><series><title level="j">Zhonghua yi xue za zhi</title><idno type="ISSN">0376-2491</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Antibodies, Viral (blood)</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>Male</term><term>Nucleocapsid Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>Male</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To establish a new technique for SARS-CoV antibody test to detect infection of severer acute respiratory syndrome (SARS).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15949361</PMID><DateCompleted><Year>2005</Year><Month>11</Month><Day>29</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0376-2491</ISSN><JournalIssue CitedMedium="Print"><Volume>85</Volume><Issue>9</Issue><PubDate><Year>2005</Year><Month>Mar</Month><Day>09</Day></PubDate></JournalIssue><Title>Zhonghua yi xue za zhi</Title><ISOAbbreviation>Zhonghua Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[An immunofluorescence assay for the detection of SARS associated coronavirus antibody based on recombinant nucleocapsid antigen and its application].</ArticleTitle><Pagination><MedlinePgn>621-4</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To establish a new technique for SARS-CoV antibody test to detect infection of severer acute respiratory syndrome (SARS).</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Nucleocapsid gene was obtained by reverse transcription and polymerase chain reaction from a SARS patient and inserted into the vector pFastBacHTa expressing baculovirus. Insect Sf9 cells were transfected with the recombinant baculovirus expressing SARS nucleocapsid antigen and then cultured, fixed by acetone so as to make SARS-specific antigen. Immunofluorescence assay (IFA) technique and plaque reduction neutralization test (PRNT) were used to detect 7 samples of sera of 4 newly diagnosed SARS patients collected in different days, 48 samples of convalescent sera of SARS patients, 24 serum samples of healthy person undergoing physical examination, and 40 serum samples from non-SARS patients with fever by double blind test.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The recombinant SARS-specific antigen reacted only with SARS positive sera but not with normal sera. Double blind test showed that 45 of the 46 PRNT positive sera were IFA positive with an accordance rate of 97.8%. 7 samples of sera from 4 SARS patients in acute progressive stage in Guangdong province were all IFA positive. SARS antibody could be detected since the sixth day after onset, and the titer increased from 1:40 to 1:600 on the ninth day.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Immunofluorescence assay is highly specific and sensitive in detection of SARS. This reagent is safe and easy to prepare.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Han-ping</ForeName><Initials>HP</Initials><AffiliationInfo><Affiliation>Institute for Virus Disease Research, Center for Disease Prevention and Control of Zhejiang Province, Hangzhou 310009, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Zhi-yong</ForeName><Initials>ZY</Initials></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Guan-mu</ForeName><Initials>GM</Initials></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Ping-ping</ForeName><Initials>PP</Initials></Author><Author ValidYN="Y"><LastName>An</LastName><ForeName>Qi</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Weng</LastName><ForeName>Jing-qing</ForeName><Initials>JQ</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Yi-yu</ForeName><Initials>YY</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Fang</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Qun-ying</ForeName><Initials>QY</Initials></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Ju-ying</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Ge</LastName><ForeName>Qiong</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Gong</LastName><ForeName>Li-ming</ForeName><Initials>LM</Initials></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Wen</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Min-hong</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhi-ya</ForeName><Initials>ZY</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Yi Xue Za Zhi</MedlineTA><NlmUniqueID>7511141</NlmUniqueID><ISSNLinking>0376-2491</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019084" MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>6</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>12</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>6</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15949361</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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