[Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells].
Identifieur interne : 002467 ( PubMed/Corpus ); précédent : 002466; suivant : 002468[Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells].
Auteurs : Jian Lu ; Bai-Ping Zhou ; Li-Xia Wen ; Xiao-Ling JiangSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Blotting, Western, Cell Line, Chlorocebus aethiops, Cloning, Molecular, Gene Expression, Humans, Neutralization Tests, Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (immunology), Peptidyl-Dipeptidase A (metabolism), Plasmids (genetics), Receptors, Virus (genetics), Receptors, Virus (immunology), Receptors, Virus (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (immunology), SARS Virus (metabolism), Transfection, Vero Cells.
- MESH :
- chemical , genetics : Peptidyl-Dipeptidase A, Receptors, Virus.
- chemical , immunology : Peptidyl-Dipeptidase A, Receptors, Virus.
- chemical , metabolism : Peptidyl-Dipeptidase A, Receptors, Virus.
- genetics : Plasmids.
- immunology : SARS Virus.
- metabolism : SARS Virus.
- Animals, Blotting, Western, Cell Line, Chlorocebus aethiops, Cloning, Molecular, Gene Expression, Humans, Neutralization Tests, Reverse Transcriptase Polymerase Chain Reaction, Transfection, Vero Cells.
Abstract
Angiotensin-converting enzyme 2 (ACE-2) has been identified as a functional receptor of severe acute respiratory syndrome coronavirus (SARS-CoV), so its gene was cloned and eukaryotic expressed for further insight into mechanisms in SARS-CoV entry and pathogenesis, as well as development of a safe and reliable neutralization assay for SARS-CoV.
PubMed: 16261211
Links to Exploration step
pubmed:16261211Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells].</title><author><name sortKey="Lu, Jian" sort="Lu, Jian" uniqKey="Lu J" first="Jian" last="Lu">Jian Lu</name><affiliation><nlm:affiliation>Shenzhen Donghu Hospital, Shenzhen 518020, China. szlujian@163.net</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Bai Ping" sort="Zhou, Bai Ping" uniqKey="Zhou B" first="Bai-Ping" last="Zhou">Bai-Ping Zhou</name></author><author><name sortKey="Wen, Li Xia" sort="Wen, Li Xia" uniqKey="Wen L" first="Li-Xia" last="Wen">Li-Xia Wen</name></author><author><name sortKey="Jiang, Xiao Ling" sort="Jiang, Xiao Ling" uniqKey="Jiang X" first="Xiao-Ling" last="Jiang">Xiao-Ling Jiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16261211</idno><idno type="pmid">16261211</idno><idno type="wicri:Area/PubMed/Corpus">002467</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002467</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells].</title><author><name sortKey="Lu, Jian" sort="Lu, Jian" uniqKey="Lu J" first="Jian" last="Lu">Jian Lu</name><affiliation><nlm:affiliation>Shenzhen Donghu Hospital, Shenzhen 518020, China. szlujian@163.net</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Bai Ping" sort="Zhou, Bai Ping" uniqKey="Zhou B" first="Bai-Ping" last="Zhou">Bai-Ping Zhou</name></author><author><name sortKey="Wen, Li Xia" sort="Wen, Li Xia" uniqKey="Wen L" first="Li-Xia" last="Wen">Li-Xia Wen</name></author><author><name sortKey="Jiang, Xiao Ling" sort="Jiang, Xiao Ling" uniqKey="Jiang X" first="Xiao-Ling" last="Jiang">Xiao-Ling Jiang</name></author></analytic><series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title><idno type="ISSN">1003-9279</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Gene Expression</term><term>Humans</term><term>Neutralization Tests</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (immunology)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Plasmids (genetics)</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (immunology)</term><term>Receptors, Virus (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (immunology)</term><term>SARS Virus (metabolism)</term><term>Transfection</term><term>Vero Cells</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Gene Expression</term><term>Humans</term><term>Neutralization Tests</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Transfection</term><term>Vero Cells</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Angiotensin-converting enzyme 2 (ACE-2) has been identified as a functional receptor of severe acute respiratory syndrome coronavirus (SARS-CoV), so its gene was cloned and eukaryotic expressed for further insight into mechanisms in SARS-CoV entry and pathogenesis, as well as development of a safe and reliable neutralization assay for SARS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16261211</PMID><DateCompleted><Year>2008</Year><Month>12</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title><ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells].</ArticleTitle><Pagination><MedlinePgn>260-3</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Angiotensin-converting enzyme 2 (ACE-2) has been identified as a functional receptor of severe acute respiratory syndrome coronavirus (SARS-CoV), so its gene was cloned and eukaryotic expressed for further insight into mechanisms in SARS-CoV entry and pathogenesis, as well as development of a safe and reliable neutralization assay for SARS-CoV.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Total RNA was extracted from right atrial tissue of a patient with right heart failure resected during a valvular replacement surgery by Trizol one-step method, and the full-length ACE-2 encoding gene was acquired by RT-nested-PCR. The ACE-2 encoding gene was then cloned into pcDNA4/HisMax-TOPO eukaryotic expression vector to construct the recombinant plasmid pcDNA4/ ACE-2, which was then transfected into 293 T cell and ACE-2 eukaryotic transient expression was detected by Western Blot. Syncytia inhibition assay was established to detect SARS-CoV neutralizing antibody, and compared parallelly with SARS pseudovirus neutralization assay.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The recombinant plasmid pcDNA4/ ACE-2 could express ACE-2 protein in eukaryotic cells and induce cell-cell fusion between S protein- and ACE2-expressing cells. This cell-cell fusion assay could be used to detect SARS-CoV neutralizing antibody.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">SARS-CoV receptor ACE-2 gene was successfully cloned and eukaryotic expressed, and used to establish syncytia inhibition assay for SARS-CoV neutralizing antibody assay.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Shenzhen Donghu Hospital, Shenzhen 518020, China. szlujian@163.net</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Bai-Ping</ForeName><Initials>BP</Initials></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Li-Xia</ForeName><Initials>LX</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Xiao-Ling</ForeName><Initials>XL</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA><NlmUniqueID>9602873</NlmUniqueID><ISSNLinking>1003-9279</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011991">Receptors, Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.15.1</RegistryNumber><NameOfSubstance UI="D007703">Peptidyl-Dipeptidase A</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.17.-</RegistryNumber><NameOfSubstance UI="C413524">angiotensin converting enzyme 2</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009500" MajorTopicYN="N">Neutralization Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007703" MajorTopicYN="N">Peptidyl-Dipeptidase A</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011991" MajorTopicYN="N">Receptors, Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>11</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>12</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>11</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16261211</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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