SrasV1, PubMed, Corpus, bibRecord, 002080

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

Identifieur interne : 002080 ( PubMed/Corpus ); précédent : 002079; suivant : 002081

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

Auteurs : Zheng Fan ; Yue Zhuo ; Xinyu Tan ; Zhi Zhou ; Jiangang Yuan ; Boqin Qiang ; Jinghua Yan ; Xiaozhong Peng ; George F. Gao

Source :

Journal of medical virology [ 0146-6615 ] ; 2006.

RBID : pubmed:16998888

English descriptors

KwdEn :
- Binding Sites, Cell Line, Humans, Nucleocapsid Proteins (metabolism), Protein Binding, SARS Virus (metabolism), Small Ubiquitin-Related Modifier Proteins (metabolism), Two-Hybrid System Techniques, Ubiquitin (metabolism), Ubiquitin-Conjugating Enzymes (metabolism).
MESH :
- chemical , metabolism : Nucleocapsid Proteins, Small Ubiquitin-Related Modifier Proteins, Ubiquitin, Ubiquitin-Conjugating Enzymes.
- metabolism : SARS Virus.
- Binding Sites, Cell Line, Humans, Protein Binding, Two-Hybrid System Techniques.

Abstract

SARS-CoV is a newly identified coronavirus (CoV) that causes severe acute respiratory syndrome (SARS). The SARS-CoV nucleocapsid (N) protein is an important structural and functional protein. To identify cellular proteins that interact with the SARS-CoV N protein and to elucidate the possible involvement of N protein in SARS-CoV pathogenesis, a human lymphocyte cDNA library was screened using a yeast two-hybrid system assay. hUbc9, a ubiquitin conjugating enzyme of sumoylation system, was found to interact specifically with the N protein, implying the post-translational sumoylation of the N protein. Mapping studies localized the critical N sequences for this interaction to amino acids 170-210, which includes the SR-rich motif. However, the consensus motif of sumoylation GK(62)EE in the N protein is not responsible for binding to hUbc9. Mutations of hUbc9 at the enzyme active site C93A or C93S severely impair the interaction with the N protein. The two proteins were also shown to colocalize in the cytoplasm of the transfected 293T cells. This is the first report demonstrating the interaction of hUbc9 with a structural protein of plus-strand RNA viruses, indicating a new drug target for SARS-CoV.

DOI: 10.1002/jmv.20707
PubMed: 16998888

Links to Exploration step

pubmed:16998888

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.</title>
<author><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name>
<affiliation><nlm:affiliation>Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zhuo, Yue" sort="Zhuo, Yue" uniqKey="Zhuo Y" first="Yue" last="Zhuo">Yue Zhuo</name>
</author>
<author><name sortKey="Tan, Xinyu" sort="Tan, Xinyu" uniqKey="Tan X" first="Xinyu" last="Tan">Xinyu Tan</name>
</author>
<author><name sortKey="Zhou, Zhi" sort="Zhou, Zhi" uniqKey="Zhou Z" first="Zhi" last="Zhou">Zhi Zhou</name>
</author>
<author><name sortKey="Yuan, Jiangang" sort="Yuan, Jiangang" uniqKey="Yuan J" first="Jiangang" last="Yuan">Jiangang Yuan</name>
</author>
<author><name sortKey="Qiang, Boqin" sort="Qiang, Boqin" uniqKey="Qiang B" first="Boqin" last="Qiang">Boqin Qiang</name>
</author>
<author><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name>
</author>
<author><name sortKey="Peng, Xiaozhong" sort="Peng, Xiaozhong" uniqKey="Peng X" first="Xiaozhong" last="Peng">Xiaozhong Peng</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16998888</idno>
<idno type="pmid">16998888</idno>
<idno type="doi">10.1002/jmv.20707</idno>
<idno type="wicri:Area/PubMed/Corpus">002080</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002080</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.</title>
<author><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name>
<affiliation><nlm:affiliation>Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Zhuo, Yue" sort="Zhuo, Yue" uniqKey="Zhuo Y" first="Yue" last="Zhuo">Yue Zhuo</name>
</author>
<author><name sortKey="Tan, Xinyu" sort="Tan, Xinyu" uniqKey="Tan X" first="Xinyu" last="Tan">Xinyu Tan</name>
</author>
<author><name sortKey="Zhou, Zhi" sort="Zhou, Zhi" uniqKey="Zhou Z" first="Zhi" last="Zhou">Zhi Zhou</name>
</author>
<author><name sortKey="Yuan, Jiangang" sort="Yuan, Jiangang" uniqKey="Yuan J" first="Jiangang" last="Yuan">Jiangang Yuan</name>
</author>
<author><name sortKey="Qiang, Boqin" sort="Qiang, Boqin" uniqKey="Qiang B" first="Boqin" last="Qiang">Boqin Qiang</name>
</author>
<author><name sortKey="Yan, Jinghua" sort="Yan, Jinghua" uniqKey="Yan J" first="Jinghua" last="Yan">Jinghua Yan</name>
</author>
<author><name sortKey="Peng, Xiaozhong" sort="Peng, Xiaozhong" uniqKey="Peng X" first="Xiaozhong" last="Peng">Xiaozhong Peng</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
</analytic>
<series><title level="j">Journal of medical virology</title>
<idno type="ISSN">0146-6615</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Binding Sites</term>
<term>Cell Line</term>
<term>Humans</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Protein Binding</term>
<term>SARS Virus (metabolism)</term>
<term>Small Ubiquitin-Related Modifier Proteins (metabolism)</term>
<term>Two-Hybrid System Techniques</term>
<term>Ubiquitin (metabolism)</term>
<term>Ubiquitin-Conjugating Enzymes (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Small Ubiquitin-Related Modifier Proteins</term>
<term>Ubiquitin</term>
<term>Ubiquitin-Conjugating Enzymes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term>
<term>Cell Line</term>
<term>Humans</term>
<term>Protein Binding</term>
<term>Two-Hybrid System Techniques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">SARS-CoV is a newly identified coronavirus (CoV) that causes severe acute respiratory syndrome (SARS). The SARS-CoV nucleocapsid (N) protein is an important structural and functional protein. To identify cellular proteins that interact with the SARS-CoV N protein and to elucidate the possible involvement of N protein in SARS-CoV pathogenesis, a human lymphocyte cDNA library was screened using a yeast two-hybrid system assay. hUbc9, a ubiquitin conjugating enzyme of sumoylation system, was found to interact specifically with the N protein, implying the post-translational sumoylation of the N protein. Mapping studies localized the critical N sequences for this interaction to amino acids 170-210, which includes the SR-rich motif. However, the consensus motif of sumoylation GK(62)EE in the N protein is not responsible for binding to hUbc9. Mutations of hUbc9 at the enzyme active site C93A or C93S severely impair the interaction with the N protein. The two proteins were also shown to colocalize in the cytoplasm of the transfected 293T cells. This is the first report demonstrating the interaction of hUbc9 with a structural protein of plus-strand RNA viruses, indicating a new drug target for SARS-CoV.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16998888</PMID>
<DateCompleted><Year>2006</Year>
<Month>12</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised><Year>2016</Year>
<Month>11</Month>
<Day>24</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0146-6615</ISSN>
<JournalIssue CitedMedium="Print"><Volume>78</Volume>
<Issue>11</Issue>
<PubDate><Year>2006</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Journal of medical virology</Title>
<ISOAbbreviation>J. Med. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.</ArticleTitle>
<Pagination><MedlinePgn>1365-73</MedlinePgn>
</Pagination>
<Abstract><AbstractText>SARS-CoV is a newly identified coronavirus (CoV) that causes severe acute respiratory syndrome (SARS). The SARS-CoV nucleocapsid (N) protein is an important structural and functional protein. To identify cellular proteins that interact with the SARS-CoV N protein and to elucidate the possible involvement of N protein in SARS-CoV pathogenesis, a human lymphocyte cDNA library was screened using a yeast two-hybrid system assay. hUbc9, a ubiquitin conjugating enzyme of sumoylation system, was found to interact specifically with the N protein, implying the post-translational sumoylation of the N protein. Mapping studies localized the critical N sequences for this interaction to amino acids 170-210, which includes the SR-rich motif. However, the consensus motif of sumoylation GK(62)EE in the N protein is not responsible for binding to hUbc9. Mutations of hUbc9 at the enzyme active site C93A or C93S severely impair the interaction with the N protein. The two proteins were also shown to colocalize in the cytoplasm of the transfected 293T cells. This is the first report demonstrating the interaction of hUbc9 with a structural protein of plus-strand RNA viruses, indicating a new drug target for SARS-CoV.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fan</LastName>
<ForeName>Zheng</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Center for Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhuo</LastName>
<ForeName>Yue</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tan</LastName>
<ForeName>Xinyu</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Zhi</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yuan</LastName>
<ForeName>Jiangang</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Qiang</LastName>
<ForeName>Boqin</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Jinghua</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Peng</LastName>
<ForeName>Xiaozhong</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gao</LastName>
<ForeName>George F</ForeName>
<Initials>GF</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Med Virol</MedlineTA>
<NlmUniqueID>7705876</NlmUniqueID>
<ISSNLinking>0146-6615</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D025841">Small Ubiquitin-Related Modifier Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D025801">Ubiquitin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.3.2.23</RegistryNumber>
<NameOfSubstance UI="D044763">Ubiquitin-Conjugating Enzymes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 6.3.2.-</RegistryNumber>
<NameOfSubstance UI="C091170">ubiquitin-conjugating enzyme UBC9</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001665" MajorTopicYN="N">Binding Sites</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D025841" MajorTopicYN="N">Small Ubiquitin-Related Modifier Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020798" MajorTopicYN="N">Two-Hybrid System Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D025801" MajorTopicYN="N">Ubiquitin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D044763" MajorTopicYN="N">Ubiquitin-Conjugating Enzymes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>9</Month>
<Day>26</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>12</Month>
<Day>9</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>9</Month>
<Day>26</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16998888</ArticleId>
<ArticleId IdType="doi">10.1002/jmv.20707</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002080 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 002080 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PubMed
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:16998888
   |texte=   SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i   -Sk "pubmed:16998888" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

SARS-CoV nucleocapsid protein binds to hUbc9, a ubiquitin conjugating enzyme of the sumoylation system.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki