Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.
Identifieur interne : 001F21 ( PubMed/Corpus ); précédent : 001F20; suivant : 001F22Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.
Auteurs : Luciano Kleber De Souza Luna ; Volker Heiser ; Nicolas Regamey ; Marcus Panning ; Jan Felix Drexler ; Sabue Mulangu ; Leo Poon ; Sigrid Baumgarte ; Bert Jan Haijema ; Laurent Kaiser ; Christian DrostenSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2007.
English descriptors
- KwdEn :
- Bacterial Typing Techniques, Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus 229E, Human (classification), Coronavirus 229E, Human (genetics), Coronavirus 229E, Human (isolation & purification), Coronavirus OC43, Human (classification), Coronavirus OC43, Human (genetics), Coronavirus OC43, Human (isolation & purification), Humans, Oligonucleotide Array Sequence Analysis (methods), RNA, Viral (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (classification), SARS Virus (genetics), SARS Virus (isolation & purification), Species Specificity.
- MESH :
- chemical , analysis : RNA, Viral.
- classification : Coronavirus, Coronavirus 229E, Human, Coronavirus OC43, Human, SARS Virus.
- genetics : Coronavirus, Coronavirus 229E, Human, Coronavirus OC43, Human, RNA, Viral, SARS Virus.
- isolation & purification : Coronavirus, Coronavirus 229E, Human, Coronavirus OC43, Human, SARS Virus.
- methods : Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.
- Bacterial Typing Techniques, Humans, Species Specificity.
Abstract
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
DOI: 10.1128/JCM.02426-06
PubMed: 17229859
Links to Exploration step
pubmed:17229859Le document en format XML
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first="Marcus" last="Panning">Marcus Panning</name></author><author><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name></author><author><name sortKey="Mulangu, Sabue" sort="Mulangu, Sabue" uniqKey="Mulangu S" first="Sabue" last="Mulangu">Sabue Mulangu</name></author><author><name sortKey="Poon, Leo" sort="Poon, Leo" uniqKey="Poon L" first="Leo" last="Poon">Leo Poon</name></author><author><name sortKey="Baumgarte, Sigrid" sort="Baumgarte, Sigrid" uniqKey="Baumgarte S" first="Sigrid" last="Baumgarte">Sigrid Baumgarte</name></author><author><name sortKey="Haijema, Bert Jan" sort="Haijema, Bert Jan" uniqKey="Haijema B" first="Bert Jan" last="Haijema">Bert Jan Haijema</name></author><author><name sortKey="Kaiser, Laurent" sort="Kaiser, Laurent" uniqKey="Kaiser L" first="Laurent" last="Kaiser">Laurent Kaiser</name></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" 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Bernhard Nocht Str. 74, 20359 Hamburg, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Heiser, Volker" sort="Heiser, Volker" uniqKey="Heiser V" first="Volker" last="Heiser">Volker Heiser</name></author><author><name sortKey="Regamey, Nicolas" sort="Regamey, Nicolas" uniqKey="Regamey N" first="Nicolas" last="Regamey">Nicolas Regamey</name></author><author><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name></author><author><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name></author><author><name sortKey="Mulangu, Sabue" sort="Mulangu, Sabue" uniqKey="Mulangu S" first="Sabue" last="Mulangu">Sabue Mulangu</name></author><author><name sortKey="Poon, Leo" sort="Poon, Leo" uniqKey="Poon L" first="Leo" last="Poon">Leo Poon</name></author><author><name sortKey="Baumgarte, Sigrid" sort="Baumgarte, Sigrid" uniqKey="Baumgarte S" first="Sigrid" last="Baumgarte">Sigrid Baumgarte</name></author><author><name sortKey="Haijema, Bert Jan" sort="Haijema, Bert Jan" uniqKey="Haijema B" first="Bert Jan" last="Haijema">Bert Jan Haijema</name></author><author><name sortKey="Kaiser, Laurent" sort="Kaiser, Laurent" uniqKey="Kaiser L" first="Laurent" last="Kaiser">Laurent Kaiser</name></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus 229E, Human (classification)</term><term>Coronavirus 229E, Human (genetics)</term><term>Coronavirus 229E, Human (isolation & purification)</term><term>Coronavirus OC43, Human (classification)</term><term>Coronavirus OC43, Human (genetics)</term><term>Coronavirus OC43, Human (isolation & purification)</term><term>Humans</term><term>Oligonucleotide Array Sequence Analysis (methods)</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (classification)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Species Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term><term>Coronavirus 229E, Human</term><term>Coronavirus OC43, Human</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term><term>Coronavirus 229E, Human</term><term>Coronavirus OC43, Human</term><term>RNA, Viral</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term><term>Coronavirus 229E, Human</term><term>Coronavirus OC43, Human</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Oligonucleotide Array Sequence Analysis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Typing Techniques</term><term>Humans</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17229859</PMID><DateCompleted><Year>2007</Year><Month>05</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>45</Volume><Issue>3</Issue><PubDate><Year>2007</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.</ArticleTitle><Pagination><MedlinePgn>1049-52</MedlinePgn></Pagination><Abstract><AbstractText>A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Souza Luna</LastName><ForeName>Luciano Kleber</ForeName><Initials>LK</Initials><AffiliationInfo><Affiliation>Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Heiser</LastName><ForeName>Volker</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Regamey</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Panning</LastName><ForeName>Marcus</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Drexler</LastName><ForeName>Jan Felix</ForeName><Initials>JF</Initials></Author><Author 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