Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS.
Identifieur interne : 001E82 ( PubMed/Corpus ); précédent : 001E81; suivant : 001E83Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS.
Auteurs : Benoît Callendret ; Valérie Lorin ; Pierre Charneau ; Philippe Marianneau ; Hugues Contamin ; Jean-Michel Betton ; Sylvie Van Der Werf ; Nicolas EscriouSource :
- Virology [ 0042-6822 ] ; 2007.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Cell Line, Enhancer Elements, Genetic (physiology), Female, Gene Expression Regulation, Genetic Vectors (genetics), Genetic Vectors (metabolism), Hepatitis B Virus, Woodchuck (genetics), Humans, Immunization, Injections, Intramuscular, Mason-Pfizer monkey virus (genetics), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred BALB C, Neutralization Tests, Plasmids (genetics), Plasmids (metabolism), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Transfection, Vaccines, DNA (administration & dosage), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, DNA, Viral Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- blood : Severe Acute Respiratory Syndrome.
- genetics : Genetic Vectors, Hepatitis B Virus, Woodchuck, Mason-Pfizer monkey virus, Plasmids, SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- metabolism : Genetic Vectors, Membrane Glycoproteins, Plasmids, SARS Virus, Viral Envelope Proteins.
- physiology : Enhancer Elements, Genetic.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Female, Gene Expression Regulation, Humans, Immunization, Injections, Intramuscular, Mice, Mice, Inbred BALB C, Neutralization Tests, Spike Glycoprotein, Coronavirus, Transfection.
Abstract
The SARS-CoV spike glycoprotein (S) is the main target of the protective immune response in humans and animal models of SARS. Here, we demonstrated that efficient expression of S from the wild-type spike gene in cultured cells required the use of improved plasmid vectors containing donor and acceptor splice sites, as well as heterologous viral RNA export elements, such as the CTE of Mazon-Pfizer monkey virus or the PRE of Woodchuck hepatitis virus (WPRE). The presence of both splice sites and WPRE markedly improved the immunogenicity of S-based DNA vaccines against SARS. Upon immunization of mice with low doses (2 microg) of naked DNA, only intron and WPRE-containing vectors could induce neutralizing anti-S antibodies and provide protection against challenge with SARS-CoV. Our observations are likely to be useful for the construction of plasmid and viral vectors designed for optimal expression of intronless genes derived from cytoplasmic RNA viruses.
DOI: 10.1016/j.virol.2007.01.012
PubMed: 17331558
Links to Exploration step
pubmed:17331558Le document en format XML
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Here, we demonstrated that efficient expression of S from the wild-type spike gene in cultured cells required the use of improved plasmid vectors containing donor and acceptor splice sites, as well as heterologous viral RNA export elements, such as the CTE of Mazon-Pfizer monkey virus or the PRE of Woodchuck hepatitis virus (WPRE). The presence of both splice sites and WPRE markedly improved the immunogenicity of S-based DNA vaccines against SARS. Upon immunization of mice with low doses (2 microg) of naked DNA, only intron and WPRE-containing vectors could induce neutralizing anti-S antibodies and provide protection against challenge with SARS-CoV. Our observations are likely to be useful for the construction of plasmid and viral vectors designed for optimal expression of intronless genes derived from cytoplasmic RNA viruses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17331558</PMID><DateCompleted><Year>2007</Year><Month>07</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>363</Volume><Issue>2</Issue><PubDate><Year>2007</Year><Month>Jul</Month><Day>05</Day></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS.</ArticleTitle><Pagination><MedlinePgn>288-302</MedlinePgn></Pagination><Abstract><AbstractText>The SARS-CoV spike glycoprotein (S) is the main target of the protective immune response in humans and animal models of SARS. Here, we demonstrated that efficient expression of S from the wild-type spike gene in cultured cells required the use of improved plasmid vectors containing donor and acceptor splice sites, as well as heterologous viral RNA export elements, such as the CTE of Mazon-Pfizer monkey virus or the PRE of Woodchuck hepatitis virus (WPRE). The presence of both splice sites and WPRE markedly improved the immunogenicity of S-based DNA vaccines against SARS. Upon immunization of mice with low doses (2 microg) of naked DNA, only intron and WPRE-containing vectors could induce neutralizing anti-S antibodies and provide protection against challenge with SARS-CoV. Our observations are likely to be useful for the construction of plasmid and viral vectors designed for optimal expression of intronless genes derived from cytoplasmic RNA viruses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Callendret</LastName><ForeName>Benoît</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA CNRS 1966, EA 302 Université Paris 7, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lorin</LastName><ForeName>Valérie</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Charneau</LastName><ForeName>Pierre</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Marianneau</LastName><ForeName>Philippe</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Contamin</LastName><ForeName>Hugues</ForeName><Initials>H</Initials></Author><Author 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