[Expression of recombinant spike protein of SARS-coronavirus in vaccinia virus and analysis of its immunogenicity].
Identifieur interne : 001D17 ( PubMed/Corpus ); précédent : 001D16; suivant : 001D18[Expression of recombinant spike protein of SARS-coronavirus in vaccinia virus and analysis of its immunogenicity].
Auteurs : Sen Hu ; Qing-Hua Wang ; Xi-Jun Wang ; Xiao-Mei Wang ; Zhi-Gao BuSource :
- Bing du xue bao = Chinese journal of virology [ 1000-8721 ] ; 2007.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Antigens, Viral (immunology), Blotting, Western, Chickens, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Gene Expression, HeLa Cells, Humans, Immune Sera (immunology), Immunization (methods), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Vaccinia virus (genetics), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Immune Sera, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- genetics : SARS Virus, Vaccinia virus.
- immunology : SARS Virus.
- methods : Immunization.
- Animals, Blotting, Western, Chickens, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Gene Expression, HeLa Cells, Humans, Mice, Spike Glycoprotein, Coronavirus.
Abstract
A recombinant vaccinia virus (rWR-SARS-S)expressing spike protein of severe acute respiratory syndrome coronavirus was constructed. The expression of full length recombinant SARS spike protein (rSS) in HeLa cells possessing specific reaction ability to chicken anti-sera was confirmed by SDS-PAGE and Western-blot (190 kD). HeLa cells infected with rWR-SARS-S also showed high sensitivity in detecting specific serum antibody by indirect immunofluoresence assay (IFA). The results above indicated that the availability of such a faithful model system offers particular advantages for the study of SARS in that it reduces the need for direct manipulation of an exotic pathogen. In the absence of infectious SARS, we may safely carry out detailed biochemical and genetic manipulations to investigate features of viral replication and gene function, as well as explore new avenue for vaccine development.
PubMed: 17894231
Links to Exploration step
pubmed:17894231Le document en format XML
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<author><name sortKey="Hu, Sen" sort="Hu, Sen" uniqKey="Hu S" first="Sen" last="Hu">Sen Hu</name>
<affiliation><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture Science, Harbin 150001, China. senhu2004@yahoo.com</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Wang, Qing Hua" sort="Wang, Qing Hua" uniqKey="Wang Q" first="Qing-Hua" last="Wang">Qing-Hua Wang</name>
</author>
<author><name sortKey="Wang, Xi Jun" sort="Wang, Xi Jun" uniqKey="Wang X" first="Xi-Jun" last="Wang">Xi-Jun Wang</name>
</author>
<author><name sortKey="Wang, Xiao Mei" sort="Wang, Xiao Mei" uniqKey="Wang X" first="Xiao-Mei" last="Wang">Xiao-Mei Wang</name>
</author>
<author><name sortKey="Bu, Zhi Gao" sort="Bu, Zhi Gao" uniqKey="Bu Z" first="Zhi-Gao" last="Bu">Zhi-Gao Bu</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Expression of recombinant spike protein of SARS-coronavirus in vaccinia virus and analysis of its immunogenicity].</title>
<author><name sortKey="Hu, Sen" sort="Hu, Sen" uniqKey="Hu S" first="Sen" last="Hu">Sen Hu</name>
<affiliation><nlm:affiliation>Harbin Veterinary Research Institute, Chinese Academy of Agriculture Science, Harbin 150001, China. senhu2004@yahoo.com</nlm:affiliation>
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<author><name sortKey="Wang, Qing Hua" sort="Wang, Qing Hua" uniqKey="Wang Q" first="Qing-Hua" last="Wang">Qing-Hua Wang</name>
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<author><name sortKey="Wang, Xi Jun" sort="Wang, Xi Jun" uniqKey="Wang X" first="Xi-Jun" last="Wang">Xi-Jun Wang</name>
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<author><name sortKey="Wang, Xiao Mei" sort="Wang, Xiao Mei" uniqKey="Wang X" first="Xiao-Mei" last="Wang">Xiao-Mei Wang</name>
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<author><name sortKey="Bu, Zhi Gao" sort="Bu, Zhi Gao" uniqKey="Bu Z" first="Zhi-Gao" last="Bu">Zhi-Gao Bu</name>
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<series><title level="j">Bing du xue bao = Chinese journal of virology</title>
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<imprint><date when="2007" type="published">2007</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antigens, Viral (immunology)</term>
<term>Blotting, Western</term>
<term>Chickens</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Fluorescent Antibody Technique, Indirect</term>
<term>Gene Expression</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Immune Sera (immunology)</term>
<term>Immunization (methods)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccinia virus (genetics)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Antigens, Viral</term>
<term>Immune Sera</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
<term>Vaccinia virus</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Chickens</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Fluorescent Antibody Technique, Indirect</term>
<term>Gene Expression</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Mice</term>
<term>Spike Glycoprotein, Coronavirus</term>
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<front><div type="abstract" xml:lang="en">A recombinant vaccinia virus (rWR-SARS-S)expressing spike protein of severe acute respiratory syndrome coronavirus was constructed. The expression of full length recombinant SARS spike protein (rSS) in HeLa cells possessing specific reaction ability to chicken anti-sera was confirmed by SDS-PAGE and Western-blot (190 kD). HeLa cells infected with rWR-SARS-S also showed high sensitivity in detecting specific serum antibody by indirect immunofluoresence assay (IFA). The results above indicated that the availability of such a faithful model system offers particular advantages for the study of SARS in that it reduces the need for direct manipulation of an exotic pathogen. In the absence of infectious SARS, we may safely carry out detailed biochemical and genetic manipulations to investigate features of viral replication and gene function, as well as explore new avenue for vaccine development.</div>
</front>
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<Month>10</Month>
<Day>17</Day>
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<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
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<Issue>4</Issue>
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<Title>Bing du xue bao = Chinese journal of virology</Title>
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<ArticleTitle>[Expression of recombinant spike protein of SARS-coronavirus in vaccinia virus and analysis of its immunogenicity].</ArticleTitle>
<Pagination><MedlinePgn>287-91</MedlinePgn>
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<Abstract><AbstractText>A recombinant vaccinia virus (rWR-SARS-S)expressing spike protein of severe acute respiratory syndrome coronavirus was constructed. The expression of full length recombinant SARS spike protein (rSS) in HeLa cells possessing specific reaction ability to chicken anti-sera was confirmed by SDS-PAGE and Western-blot (190 kD). HeLa cells infected with rWR-SARS-S also showed high sensitivity in detecting specific serum antibody by indirect immunofluoresence assay (IFA). The results above indicated that the availability of such a faithful model system offers particular advantages for the study of SARS in that it reduces the need for direct manipulation of an exotic pathogen. In the absence of infectious SARS, we may safely carry out detailed biochemical and genetic manipulations to investigate features of viral replication and gene function, as well as explore new avenue for vaccine development.</AbstractText>
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